{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,26]],"date-time":"2026-08-26T19:37:37Z","timestamp":1787773057847,"version":"build-2784847793"},"reference-count":31,"publisher":"Wiley","issue":"5-6","license":[{"start":{"date-parts":[[2013,5,1]],"date-time":"2013-05-01T00:00:00Z","timestamp":1367366400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Parasite Immunology"],"published-print":{"date-parts":[[2013,5]]},"abstract":"Summary<\/jats:title>Enzyme\u2010linked immunosorbent assays (ELISAs) were developed for the detection of I<\/jats:styled-content>gG<\/jats:styled-content>, I<\/jats:styled-content>gG<\/jats:styled-content>4<\/jats:sub> and I<\/jats:styled-content>gE<\/jats:styled-content> antibodies against S<\/jats:styled-content>trongyloides stercoralis<\/jats:italic>. A commercial ELISA<\/jats:styled-content> (IVD<\/jats:styled-content> Research, USA) was also used, and the sensitivities and specificities of the four assays were determined. Serum samples from 26 patients with S<\/jats:styled-content>.\u00a0stercoralis<\/jats:italic> infection and 55 patients with other infections or no infection were analysed. Sensitivities of the I<\/jats:styled-content>gG<\/jats:styled-content>4<\/jats:sub>, I<\/jats:styled-content>gG<\/jats:styled-content>, I<\/jats:styled-content>gE<\/jats:styled-content> and I<\/jats:styled-content>gG<\/jats:styled-content> (IVD<\/jats:styled-content>) assays were 76\u00b79%, 84\u00b76%, 7\u00b77% and 84\u00b76%, respectively, while the specificities were 92\u00b77%, 81\u00b78%, 100% and 83\u00b76%, respectively. If filariasis samples were excluded, the specificities of the I<\/jats:styled-content>gG<\/jats:styled-content>4<\/jats:sub>\u2010ELISA<\/jats:styled-content> and both I<\/jats:styled-content>gG<\/jats:styled-content>\u2010ELISA<\/jats:styled-content>s increased to 100% and 98%, respectively. A significant positive correlation was observed between I<\/jats:styled-content>gG<\/jats:styled-content>\u2010 and I<\/jats:styled-content>gG<\/jats:styled-content>4<\/jats:sub>\u2010ELISA<\/jats:styled-content>s (r<\/jats:italic>\u00a0=\u00a00\u00b74828; P<\/jats:italic>\u00a0=\u00a00\u00b70125). I<\/jats:styled-content>gG<\/jats:styled-content>\u2010 and I<\/jats:styled-content>gG<\/jats:styled-content>\u2010 (IVD<\/jats:styled-content>) ELISA<\/jats:styled-content>s (r<\/jats:italic>\u00a0=\u00a00\u00b7309) were positively correlated, but was not significant (P<\/jats:italic>\u00a0=\u00a00\u00b7124). Meanwhile there was no correlation between I<\/jats:styled-content>gG<\/jats:styled-content>4<\/jats:sub>\u2010 and I<\/jats:styled-content>gG<\/jats:styled-content>\u2010 (IVD<\/jats:styled-content>) ELISA<\/jats:styled-content>s (r<\/jats:italic>\u00a0=\u00a00\u00b70042; P<\/jats:italic>\u00a0=\u00a00\u00b78294). Sera from brugian filariasis patients showed weak, positive correlation between the titres of antifilarial IgG4<\/jats:sub> and the optical densities of anti\u2010Strongyloides <\/jats:italic>I<\/jats:styled-content>gG<\/jats:styled-content>4<\/jats:sub>\u2010ELISA<\/jats:styled-content> (r<\/jats:italic>\u00a0=\u00a00\u00b74544, P<\/jats:italic>\u00a0=\u00a00\u00b70294). In conclusion, the detection of both anti\u2010Strongyloides <\/jats:italic>I<\/jats:styled-content>gG<\/jats:styled-content>4<\/jats:sub> and I<\/jats:styled-content>gG<\/jats:styled-content> antibodies could improve the serodiagnosis of human strongyloidiasis. Furthermore, patients from lymphatic filariasis endemic areas who are serologically diagnosed with strongyloidiasis should also be tested for filariasis.<\/jats:p>","DOI":"10.1111\/pim.12029","type":"journal-article","created":{"date-parts":[[2013,3,1]],"date-time":"2013-03-01T07:29:25Z","timestamp":1362122965000},"page":"174-179","source":"Crossref","is-referenced-by-count":33,"title":["Laboratory detection of strongyloidiasis: I<\/scp>gG<\/scp>\u2010, I<\/scp>gG<\/scp>4<\/sub>\u2010 and I<\/scp>gE<\/scp>\u2010ELISA<\/scp>s and cross\u2010reactivity with lymphatic filariasis"],"prefix":"10.1111","volume":"35","author":[{"given":"A.","family":"Norsyahida","sequence":"first","affiliation":[{"name":"Institute for Research in Molecular Medicine Universiti Sains Malaysia Minden Penang Malaysia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Riazi","sequence":"additional","affiliation":[{"name":"School of Pharmaceutical Sciences Universiti Sains Malaysia Minden Penang Malaysia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S. M.","family":"Sadjjadi","sequence":"additional","affiliation":[{"name":"Shiraz University of Medical Sciences Shiraz Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Y.","family":"Muhammad Hafiznur","sequence":"additional","affiliation":[{"name":"Institute for Research in Molecular Medicine Universiti Sains Malaysia Minden Penang Malaysia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H. 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