{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,13]],"date-time":"2026-02-13T09:18:13Z","timestamp":1770974293800,"version":"3.50.1"},"reference-count":27,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2014,4,8]],"date-time":"2014-04-08T00:00:00Z","timestamp":1396915200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Parasite Immunology"],"published-print":{"date-parts":[[2014,5]]},"abstract":"Summary<\/jats:title>An attenuated line of L<\/jats:styled-content>eishmania infantum<\/jats:italic> (the H\u2010line), developed through exposure to gentamicin, has been shown to protect dogs against canine visceral leishmaniasis. A specific diagnostic test to differentiate dogs vaccinated with the attenuated line from dogs infected with L<\/jats:styled-content>.\u00a0infantum<\/jats:italic> wild\u2010type (L<\/jats:styled-content>.\u00a0infantum <\/jats:italic>WT<\/jats:styled-content>) could be a valuable tool in evaluating the effectiveness of canine vaccination. In this study, 28 healthy dogs were allocated into four groups. In Group I and Group II (eight dogs per group), dogs were immunized subcutaneously (s.c.) with L<\/jats:styled-content>.\u00a0infantum<\/jats:italic> H\u2010line, and the dogs of Group II challenged s.c. with L<\/jats:styled-content>.\u00a0infantum <\/jats:italic>WT<\/jats:styled-content>, at 2\u00a0months post\u2010immunization. In Group III, eight animals were challenged s.c. with L<\/jats:styled-content>.\u00a0infantum <\/jats:italic>WT<\/jats:styled-content>, and four dogs of Group IV were injected s.c. with PBS<\/jats:styled-content>. We found that sera from vaccinated dogs recognize a 21\u00a0kDa antigen of promastigotes of L<\/jats:styled-content>.\u00a0infantum<\/jats:italic> H\u2010line but not of L<\/jats:styled-content>.\u00a0infantum <\/jats:italic>WT<\/jats:styled-content>, whereas sera from unvaccinated dogs challenged with L<\/jats:styled-content>.\u00a0infantum <\/jats:italic>WT<\/jats:styled-content>, recognized a 21\u00a0kDa antigen of promastigotes of L<\/jats:styled-content>.\u00a0infantum <\/jats:italic>WT<\/jats:styled-content> but not of L<\/jats:styled-content>.\u00a0infantum<\/jats:italic> H\u2010line. Sera from dogs challenged with L<\/jats:styled-content>.\u00a0infantum <\/jats:italic>WT<\/jats:styled-content> with prior vaccination with L<\/jats:styled-content>.\u00a0infantum<\/jats:italic> H\u2010line, recognized a 21\u00a0kDa antigen of both L<\/jats:styled-content>.\u00a0infantum <\/jats:italic>WT<\/jats:styled-content> and L<\/jats:styled-content>.\u00a0infantum<\/jats:italic> H\u2010line. These results suggest that the Western blot analysis of antibodies against 21\u00a0kDa antigens of L<\/jats:styled-content>.\u00a0infantum<\/jats:italic> H\u2010line and WT<\/jats:styled-content> may be a useful technique for distinguishing between dogs vaccinated with L<\/jats:styled-content>.\u00a0infantum<\/jats:italic> H\u2010line and dogs naturally infected with L<\/jats:styled-content>.\u00a0infantum <\/jats:italic>WT<\/jats:styled-content>.<\/jats:p>","DOI":"10.1111\/pim.12108","type":"journal-article","created":{"date-parts":[[2014,2,24]],"date-time":"2014-02-24T18:23:39Z","timestamp":1393266219000},"page":"218-224","source":"Crossref","is-referenced-by-count":4,"title":["Dogs vaccinated with gentamicin\u2010attenuated L<\/scp>eishmania infantum<\/i> or infected with wild\u2010type parasite can be distinguished by Western blotting"],"prefix":"10.1111","volume":"36","author":[{"given":"H.","family":"Daneshvar","sequence":"first","affiliation":[{"name":"Leishmaniasis Center Kerman University of Medical Sciences Kerman Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Z.","family":"Mahmmodi","sequence":"additional","affiliation":[{"name":"Immunology Department Kerman Medical University Kerman Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H.","family":"Kamiabi","sequence":"additional","affiliation":[{"name":"Research Center of Tropical and Infectious Diseases Kerman University of Medical Sciences Kerman Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R. S.","family":"Phillips","sequence":"additional","affiliation":[{"name":"School of Life Sciences College of Medical, Veterinary and Life Sciences University of Glasgow Glasgow UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R.","family":"Burchmore","sequence":"additional","affiliation":[{"name":"Institute of Infection, Immunity and Inflammation College of Medical, Veterinary and Life Sciences University of Glasgow Glasgow UK"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2014,4,8]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1093\/clinids\/22.1.1"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.2001.65.510"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-3156.2001.00778.x"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0001-706X(01)00154-1"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1086\/374783"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jprot.2011.11.018"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijpara.2008.11.009"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0891-5849(02)01088-2"},{"key":"e_1_2_5_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-3024.2010.01237.x"},{"key":"e_1_2_5_11_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0002757"},{"key":"e_1_2_5_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/0035-9203(95)90447-6"},{"key":"e_1_2_5_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4894(90)90031-7"},{"key":"e_1_2_5_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0304-4017(00)00436-2"},{"key":"e_1_2_5_15_1","doi-asserted-by":"publisher","DOI":"10.1006\/expr.1996.0018"},{"key":"e_1_2_5_16_1","doi-asserted-by":"publisher","DOI":"10.1177\/104063879901100211"},{"key":"e_1_2_5_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetimm.2008.12.002"},{"key":"e_1_2_5_18_1","first-page":"296","article-title":"The FML\u2010ELISA assay in diagnosis and prognosis of canine visceral leishmaniasis","volume":"6","author":"Borja\u2010Cabrera GP","year":"1999","journal-title":"Am J Trop Med Hyg"},{"key":"e_1_2_5_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0264-410X(03)00046-X"},{"key":"e_1_2_5_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2004.11.061"},{"key":"e_1_2_5_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.imlet.2005.04.006"},{"key":"e_1_2_5_22_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0000842"},{"key":"e_1_2_5_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molbiopara.2005.10.008"},{"key":"e_1_2_5_24_1","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.1998.58.154"},{"key":"e_1_2_5_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/0035-9203(94)90214-3"},{"key":"e_1_2_5_26_1","doi-asserted-by":"crossref","first-page":"772","DOI":"10.4269\/ajtmh.1992.47.772","article-title":"Serodiagnosis and screening of canine visceral leishmaniasis in endemic area of Corsica: applicability of a direct agglutination test and immunoblot analysis","volume":"47","author":"Neogy AB","year":"1992","journal-title":"Am J Trop Med Hyg"},{"key":"e_1_2_5_27_1","doi-asserted-by":"publisher","DOI":"10.1590\/S0037-86821996000200008"},{"key":"e_1_2_5_28_1","doi-asserted-by":"publisher","DOI":"10.1128\/CVI.00133-07"}],"container-title":["Parasite Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fpim.12108","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/pim.12108","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,29]],"date-time":"2023-09-29T23:46:52Z","timestamp":1696031212000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/pim.12108"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,4,8]]},"references-count":27,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2014,5]]}},"alternative-id":["10.1111\/pim.12108"],"URL":"https:\/\/doi.org\/10.1111\/pim.12108","archive":["Portico"],"relation":{},"ISSN":["0141-9838","1365-3024"],"issn-type":[{"value":"0141-9838","type":"print"},{"value":"1365-3024","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,4,8]]}}}