{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,15]],"date-time":"2026-05-15T19:06:30Z","timestamp":1778871990671,"version":"3.51.4"},"reference-count":32,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2014,6,17]],"date-time":"2014-06-17T00:00:00Z","timestamp":1402963200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Instituto Nacional de Ci\u00ef\u00bf\u00bdncia e Tecnologia de Doen\u00ef\u00bf\u00bdas Tropicais"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Parasite Immunology"],"published-print":{"date-parts":[[2014,7]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>Serodiagnosis of visceral leishmaniasis is often hindered by cross\u2010reactions to other parasitic diseases. Identifying specific <jats:styled-content style=\"fixed-case\">B<\/jats:styled-content>\u2010cell epitopes in proteins is therefore important for immunodiagnostics, as well as for disease control by vaccines. This study aimed to identify linear and conformational <jats:styled-content style=\"fixed-case\">B<\/jats:styled-content>\u2010cell epitopes and to evaluate the secondary structure of antigen proteins in <jats:italic>Leishmania infantum<\/jats:italic> using <jats:italic>in silico<\/jats:italic> analysis. Linear epitopes were predicted using the Immune Epitope Database and Analysis Resource (<jats:styled-content style=\"fixed-case\">IEDB<\/jats:styled-content>), BepiPred and BcePred programs. The conformational <jats:styled-content style=\"fixed-case\">B<\/jats:styled-content>\u2010cell epitopes were identified using the <jats:styled-content style=\"fixed-case\">CBTOPE<\/jats:styled-content> server. The combination of the predictions using <jats:styled-content style=\"fixed-case\">IEDB<\/jats:styled-content>, BepiPred and BcePred generated 148 linear epitopes from the calpain\u2010like cysteine peptidase (<jats:styled-content style=\"fixed-case\">CP<\/jats:styled-content>), thiol\u2010dependent reductase 1 (<jats:styled-content style=\"fixed-case\">TDR<\/jats:styled-content>1) and <jats:styled-content style=\"fixed-case\">HSP<\/jats:styled-content>70 proteins. In total, 164 conformational epitopes were predicted, mostly located in the linear epitope region. The predicted epitopes are located in \u03b1 helix and random coil regions in the thiol\u2010dependent reductase 1 and <jats:styled-content style=\"fixed-case\">HSP<\/jats:styled-content>70 proteins. New linear and conformational <jats:styled-content style=\"fixed-case\">B<\/jats:styled-content>\u2010cell epitopes of <jats:italic>L.\u00a0infantum<\/jats:italic> proteins were identified <jats:italic>in silico<\/jats:italic>, and the prediction using various programs ensures greater accuracy of the results, as suggested by confirmation of previously identified <jats:styled-content style=\"fixed-case\">HSP<\/jats:styled-content>70 epitopes.<\/jats:p>","DOI":"10.1111\/pim.12111","type":"journal-article","created":{"date-parts":[[2014,3,10]],"date-time":"2014-03-10T07:02:40Z","timestamp":1394434960000},"page":"313-323","source":"Crossref","is-referenced-by-count":23,"title":["B\u2010cell epitopes of antigenic proteins in <i>Leishmania infantum<\/i>: an <i>in silico<\/i> analysis"],"prefix":"10.1111","volume":"36","author":[{"given":"L. M.","family":"Assis","sequence":"first","affiliation":[{"name":"Graduate Program in Medicine and Health Professor Edgard Santos University Hospital Complex Federal University of Bahia (Universidade Federal da Bahia\u2010UFBA) Salvador Brazil"},{"name":"Academic Nursing Unit Federal University of Campina Grande (Universidade Federal de Campina Grande)\u2010UFCG Cajazeiras Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J. R.","family":"Sousa","sequence":"additional","affiliation":[{"name":"Santa Maria College (Faculdade Santa Maria\u2010FSM) Cajazeiras Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"N. F. S.","family":"Pinto","sequence":"additional","affiliation":[{"name":"Graduate Program in Veterinary Medicine Veterinary Medicine Academic Unit Federal University of Campina Grande\u2010UFCG Patos Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A. A.","family":"Silva","sequence":"additional","affiliation":[{"name":"Academic Unit of Exact and Natural Sciences Federal University of Campina Grande Cajazeiras Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A. F. M.","family":"Vaz","sequence":"additional","affiliation":[{"name":"Academic Unit of Veterinary Medicine Federal University of Campina Grande\u2010UFCG Patos Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P. P.","family":"Andrade","sequence":"additional","affiliation":[{"name":"Department of Genetics Federal University of Pernambuco\u2010UFPE Recife Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"E. M.","family":"Carvalho","sequence":"additional","affiliation":[{"name":"Graduate Program in Medicine and Health, Immunology Service Professor Edgard Santos University Hospital Complex Federal University of Bahia Salvador Brazil"},{"name":"National Institute of Science and Technology of Tropical Diseases (Instituto Nacional de Ci\u00eancia e Tecnologia de Doen\u00e7as Tropicais) \u2010 INCT DT(CNPq\/MCT) Salvador Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M. 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