{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T03:02:28Z","timestamp":1790218948532,"version":"4.1.0"},"reference-count":61,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2014,11,27]],"date-time":"2014-11-27T00:00:00Z","timestamp":1417046400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Parasite Immunology"],"published-print":{"date-parts":[[2014,12]]},"abstract":"<jats:title>Summary<\/jats:title>\n                  <jats:p>\n                    Much remains unknown about the mammalian immune response to\n                    <jats:italic>Giardia lamblia<\/jats:italic>\n                    , a protozoan pathogen that causes diarrhoeal outbreaks. We fractionated protein extracts of\n                    <jats:italic>G.\u00a0lamblia<\/jats:italic>\n                    trophozoites by Viva\u2010spin centrifugation, DEAE ion exchange and gel filtration chromatography. Resultant fractions were screened for antigenic molecules by western blots analysis using anti\u2010\n                    <jats:italic>G.\u00a0lamblia<\/jats:italic>\n                    antibodies (Abs), resulting in identification of\n                    <jats:italic>G.\u00a0lamblia<\/jats:italic>\n                    binding immunoglobulin protein (GlBiP). Maturation of mouse dendritic cells (DCs) in response to recombinant GlBiP (\n                    <jats:styled-content style=\"fixed-case\">rGlBiP<\/jats:styled-content>\n                    ) was detected by increased expression of surface molecules such as CD80, CD86 and MHC class II; these mature DCs, produced pro\u2010inflammatory cytokines (TNF\u2010\u03b1, IL\u201012 and IL\u20106). Especially, the truncated\n                    <jats:styled-content style=\"fixed-case\">rGlBiP<\/jats:styled-content>\n                    containing the heat\u2010shock protein 70 domain\u2010induced cytokine production from mouse DCs.\n                    <jats:styled-content style=\"fixed-case\">rGlBiP<\/jats:styled-content>\n                    \u2010induced DC activation was initiated by TLR4 in a MyD88\u2010dependent way and occurred through activation of p38 and ERK1\/2 MAPKs as well as increased activity of NF\u2010\n                    <jats:styled-content style=\"fixed-case\">\u03baB<\/jats:styled-content>\n                    and AP\u20101. Moreover, CD4\n                    <jats:sup>+<\/jats:sup>\n                    T cells stimulated with\n                    <jats:styled-content style=\"fixed-case\">rGlBiP<\/jats:styled-content>\n                    \u2010treated DCs produced high levels of IL\u20102 and IFN\u2010\u03b3. Together, our results suggest that GlBiP contributes to maturation of DCs via activation of TLR4\u2010MyD88\u2010p38, ERK1\/2 MAPK, NF\u2010\u03baB and AP\u20101.\n                  <\/jats:p>","DOI":"10.1111\/pim.12119","type":"journal-article","created":{"date-parts":[[2014,5,28]],"date-time":"2014-05-28T07:16:50Z","timestamp":1401261410000},"page":"627-646","source":"Crossref","is-referenced-by-count":29,"title":["<i>Giardia lamblia<\/i>\n                    binding immunoglobulin protein triggers maturation of dendritic cells via activation of\n                    <scp>TLR<\/scp>\n                    4\u2010MyD88\u2010p38 and\n                    <scp>ERK<\/scp>\n                    1\/2\n                    <scp>MAPK<\/scp>\n                    s"],"prefix":"10.1111","volume":"36","author":[{"given":"H.\u2010Y.","family":"Lee","sequence":"first","affiliation":[{"name":"Department of Environmental Medical Biology and Institute of Tropical Medicine Brain Korea Plus Project for Medical Science Yonsei University College of Medicine Seoul South Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Environmental Medical Biology and Institute of Tropical Medicine Brain Korea Plus Project for Medical Science Yonsei University College of Medicine Seoul South Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H. 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