{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,7]],"date-time":"2025-11-07T13:21:22Z","timestamp":1762521682319},"reference-count":41,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2014,9,17]],"date-time":"2014-09-17T00:00:00Z","timestamp":1410912000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Department of biotechnology, Govt of India","award":["BT\/01\/COE\/06\/13"],"award-info":[{"award-number":["BT\/01\/COE\/06\/13"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Parasite Immunology"],"published-print":{"date-parts":[[2014,10]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>The chromadorea abundant larval transcript (<jats:styled-content style=\"fixed-case\">ALT<\/jats:styled-content>) family of proteins contains <jats:styled-content style=\"fixed-case\">ALT<\/jats:styled-content> one of the most studied putative vaccine candidate in experimental filariasis. This study reports the characterization of <jats:italic>Wuchereria bancrofti<\/jats:italic> 20\/22 (Wb20\/22) as a member of chromadorea, the <jats:styled-content style=\"fixed-case\">ALT<\/jats:styled-content> family of proteins from the L3 stage of <jats:italic>W.\u00a0bancrofti<\/jats:italic>. The high reactivity with serum from the endemic normal (<jats:styled-content style=\"fixed-case\">EN<\/jats:styled-content>) population suggests that Wb20\/22 could be a target of elicit protective immunity. The glutamic acid\u2010rich region of Wb20\/22 was predicted to harbour the longest linear B\u2010cell epitope by insilico prediction tools. The significance of this region was revealed by studying the mutant form of Wb20\/22, without acidic domain (<jats:styled-content style=\"fixed-case\">WOAD<\/jats:styled-content>) which was cloned, and the immune response was compared with Wb20\/22. The signal sequence of Wb20\/22 was also an immunodominant region, and mutant construct without signal sequence (<jats:styled-content style=\"fixed-case\">WOSS<\/jats:styled-content>) was cloned and characterized. The peak antibody titre elicited by <jats:styled-content style=\"fixed-case\">WOAD<\/jats:styled-content> was higher than Wb20\/22 or <jats:styled-content style=\"fixed-case\">WOSS<\/jats:styled-content>, which pointed to the immunomodulatory role of glutamic acid\u2010rich region. Wb20\/22 elicited very high levels of <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u201010 and diminished levels of <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u20104 and <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u20105 which could be the reason for low antibody titre. The prophylactic efficacy of <jats:styled-content style=\"fixed-case\">WOAD<\/jats:styled-content> conferred protection (62\u00b726%) which was higher than Wb20\/22 (49\u00b782%) and <jats:styled-content style=\"fixed-case\">WOSS<\/jats:styled-content> (54\u00b778%).<\/jats:p>","DOI":"10.1111\/pim.12120","type":"journal-article","created":{"date-parts":[[2014,6,2]],"date-time":"2014-06-02T16:22:45Z","timestamp":1401726165000},"page":"475-484","source":"Crossref","is-referenced-by-count":6,"title":["<i>Wuchereria bancrofti<\/i> 20\/22 a homologue of abundant larval transcript L3 stage filarial antigen: molecular and immunological characterization"],"prefix":"10.1111","volume":"36","author":[{"given":"Ramanathan","family":"Aparnaa","sequence":"first","affiliation":[{"name":"Centre for Biotechnology Anna University, Guindy Chennai 600025 Tamilnadu India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Natarajan","family":"Mahalakshmi","sequence":"additional","affiliation":[{"name":"Centre for Biotechnology Anna University, Guindy Chennai 600025 Tamilnadu India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Asai","family":"Harini","sequence":"additional","affiliation":[{"name":"Centre for Biotechnology Anna University, Guindy Chennai 600025 Tamilnadu India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Parasurama Jawaharlal","family":"Jeyaprita","sequence":"additional","affiliation":[{"name":"Centre for Biotechnology Anna University, Guindy Chennai 600025 Tamilnadu India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gandhirajan","family":"Anugraha","sequence":"additional","affiliation":[{"name":"Centre for Biotechnology Anna University, Guindy Chennai 600025 Tamilnadu India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"N. 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