{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,20]],"date-time":"2026-09-20T03:10:55Z","timestamp":1789873855563,"version":"4.0.1"},"reference-count":55,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2014,9,17]],"date-time":"2014-09-17T00:00:00Z","timestamp":1410912000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Parasite Immunology"],"published-print":{"date-parts":[[2014,10]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>Traditionally serum and\/or <jats:styled-content style=\"fixed-case\">CSF<\/jats:styled-content> specimens have been used for detection of either specific antibodies or antigens as a supportive diagnosis of <jats:styled-content style=\"fixed-case\">NCC<\/jats:styled-content>. However, in recent days, much interest has been shown employing noninvasive specimens such as urine. In our study, we identified and compared a profile of circulating antigenic peptides of parasite origin in three different body fluids (<jats:styled-content style=\"fixed-case\">CSF<\/jats:styled-content>, serum and urine) obtained from confirmed <jats:styled-content style=\"fixed-case\">NCC<\/jats:styled-content> cases and control subjects. The circulating antigenic peptides were resolved by <jats:styled-content style=\"fixed-case\">SDS<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">PAGE<\/jats:styled-content> and subjected to immunoblotting. For confirmation of their origin as parasite somatic or excretory secretory (<jats:styled-content style=\"fixed-case\">ES<\/jats:styled-content>) material, immunoreactivity was tested employing affinity purified polyclonal <jats:italic>Taenia solium<\/jats:italic> metacestode anti\u2010somatic or <jats:styled-content style=\"fixed-case\">ES<\/jats:styled-content> antibodies, respectively. Only lower molecular weight antigenic peptides were found circulating in urine in contrast to serum and <jats:styled-content style=\"fixed-case\">CSF<\/jats:styled-content> specimens. Few somatic peptides were identified to be 100% specific for <jats:styled-content style=\"fixed-case\">NCC<\/jats:styled-content> (19\u00b75\u00a0kDa in all three specimens; 131, 70\u00a0kDa in <jats:styled-content style=\"fixed-case\">CSF<\/jats:styled-content> and serum only; 128\u00a0kDa in <jats:styled-content style=\"fixed-case\">CSF<\/jats:styled-content> only). Similarly, the specific <jats:styled-content style=\"fixed-case\">ES<\/jats:styled-content> peptides detected were 32\u00a0kDa (in all three specimens), 16\u00b75\u00a0kDa (in serum and <jats:styled-content style=\"fixed-case\">CSF<\/jats:styled-content> only), and 15\u00a0kDa (urine only). A test format detecting either one or more of these specific peptides would enhance the sensitivity in diagnosis of <jats:styled-content style=\"fixed-case\">NCC<\/jats:styled-content>.<\/jats:p>","DOI":"10.1111\/pim.12124","type":"journal-article","created":{"date-parts":[[2014,6,26]],"date-time":"2014-06-26T07:22:05Z","timestamp":1403767325000},"page":"509-521","source":"Crossref","is-referenced-by-count":4,"title":["Comparative profile of circulating antigenic peptides in <scp>CSF<\/scp>, serum &amp; urine from patients with neurocysticercosis diagnosed by immunoblotting"],"prefix":"10.1111","volume":"36","author":[{"given":"P. S.","family":"Sahu","sequence":"first","affiliation":[{"name":"Division of Pathology School of Medicine International Medical University Kuala Lumpur Malaysia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S.","family":"Parija","sequence":"additional","affiliation":[{"name":"Division of Pathology School of Medicine International Medical University Kuala Lumpur Malaysia"},{"name":"Department of Microbiology Jawaharlal Institute of Postgraduate Medical Education and Research Pondicherry India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D.","family":"Kumar","sequence":"additional","affiliation":[{"name":"Department of Parasitology Rajiv Gandhi College of Veterinary and Animal Sciences Pondicherry India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S.","family":"Jayachandran","sequence":"additional","affiliation":[{"name":"School of Biotechnology Pondicherry University Pondicherry 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