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Mice were intramuscularly immunized with <jats:styled-content style=\"fixed-case\">pTgMDH<\/jats:styled-content>, followed by challenge with virulent <jats:italic>T.\u00a0gondii<\/jats:italic> RH strain, 2\u00a0weeks after the booster immunization. Compared to the control groups, the results showed that <jats:styled-content style=\"fixed-case\">pTgMDH<\/jats:styled-content> has stimulated specific humoral response as demonstrated by significant high titers of total IgG and subclasses IgG<jats:sub>1<\/jats:sub> and IgG<jats:sub>2a<\/jats:sub>, beside IgA and IgM, but not IgE. Analysis of cytokine profiles revealed significant increases of IFN\u2010\u03b3, IL\u20104 and IL\u201017, while no significant changes were detected in TGF\u2010\u03b21. In cell\u2010mediated response, both T lymphocytes subpopulations CD4<jats:sup>+<\/jats:sup> and CD8<jats:sup>+<\/jats:sup> were positively recruited as significant percentages were recorded in response to immunization with TgMDH. Significant long survival rate, 17\u00a0days, has been observed in the TgMDH vaccinated group, in contrast with control groups which died within 8\u20139\u00a0days after challenge. These results demonstrated that TgMDH could induce significant immunological responses leading to a considerable level of protection against acute toxoplasmosis infection.<\/jats:p>","DOI":"10.1111\/pim.12146","type":"journal-article","created":{"date-parts":[[2014,9,19]],"date-time":"2014-09-19T15:58:52Z","timestamp":1411142332000},"page":"674-683","source":"Crossref","is-referenced-by-count":16,"title":["Immunological response and protection of mice immunized with plasmid encoding <i>Toxoplasma gondii<\/i> glycolytic enzyme malate dehydrogenase"],"prefix":"10.1111","volume":"36","author":[{"given":"I. 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