{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T16:35:45Z","timestamp":1789749345730,"version":"4.0.1"},"reference-count":11,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2014,12,29]],"date-time":"2014-12-29T00:00:00Z","timestamp":1419811200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Parasite Immunology"],"published-print":{"date-parts":[[2015,1]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>Clinical diagnosis and post\u2010surgery assessment of cystic echinococcosis depend on laboratory serodiagnosis and ultrasound examinations. This study aims to produce the recombinant antigen (<jats:styled-content style=\"fixed-case\">rAgB<\/jats:styled-content>) and compare its diagnostic effect with natural antigens (crude fluid antigen, protoscolex antigen). After <jats:styled-content style=\"fixed-case\">rAgB<\/jats:styled-content>, crude fluid antigen, protoscolex antigen were produced, and the diagnostic accuracy was evaluated with dot immunogold filtration assay (DIGFA) by the sera from the following groups: surgically confirmed cystic echinococcosis patients (<jats:italic>n<\/jats:italic>\u00a0=\u00a0113), alveolar echinococcosis patients (<jats:italic>n<\/jats:italic>\u00a0=\u00a046), other parasitic diseases (<jats:italic>n<\/jats:italic>\u00a0=\u00a049), nonparasitic hepatic diseases (<jats:italic>n<\/jats:italic>\u00a0=\u00a063) and healthy people (<jats:italic>n<\/jats:italic>\u00a0=\u00a0121). In diagnosing cystic echinococcosis, the sensitivity of recombinant AgB was 77\u00b79% and the specificity was 98\u00b73%. The crude fluid antigen B showed a sensitivity of 92\u00b79% and specificity of 81\u00b70%. The protoscolex antigen had sensitivity of 87\u00b76% and specificity of 90\u00b79%. The recombinant AgB indicates the advantage of no cross\u2010reaction with other parasite diseases or nonparasite hepatic diseases. Recombinant antigen B can improve the specificity but decrease the sensitivity. The combination of native and recombinant antigens will improve the overall performance of serodiagnosis of cystic echinococcosis.<\/jats:p>","DOI":"10.1111\/pim.12151","type":"journal-article","created":{"date-parts":[[2014,10,14]],"date-time":"2014-10-14T12:12:19Z","timestamp":1413288739000},"page":"10-15","source":"Crossref","is-referenced-by-count":14,"title":["The production and comparative evaluation of native and recombinant antigens for the fast serodiagnosis of cystic echinococcosis with dot immunogold filtration assay"],"prefix":"10.1111","volume":"37","author":[{"given":"X.","family":"Chen","sequence":"first","affiliation":[{"name":"The Department of Hepatobiliary and Pancreatic Surgery the First Affiliated Hospital Zhejiang University School of Medicine Hangzhou Zhejiang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"X.","family":"Chen","sequence":"additional","affiliation":[{"name":"The Department of Pharmacy Shandong University of Traditional Chinese Medicine Jinan Shandong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"X.","family":"Lu","sequence":"additional","affiliation":[{"name":"The State Key Laboratory Incubation Base of Xinjiang Major Diseases and Xinjiang Key Laboratory of Echinococcosis The First Affiliated Hospital of Xinjiang Medical University Urumqi Xinjiang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"X.","family":"Feng","sequence":"additional","affiliation":[{"name":"The State Key Laboratory Incubation Base of Xinjiang Major Diseases and Xinjiang Key Laboratory of Echinococcosis The First Affiliated Hospital of Xinjiang Medical University Urumqi Xinjiang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H.","family":"Wen","sequence":"additional","affiliation":[{"name":"The State Key Laboratory Incubation Base of Xinjiang Major Diseases and Xinjiang Key Laboratory of Echinococcosis The First Affiliated Hospital of Xinjiang Medical University Urumqi Xinjiang China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2014,12,29]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0002937"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00436-014-3947-0"},{"key":"e_1_2_7_4_1","article-title":"Epidemiology and control of echinococcosis in central Asia, with particular reference to the People's Republic of China","author":"Zhang W","year":"2014","journal-title":"Acta Trop"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.actatropica.2009.10.003"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/pim.12096"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/ng.2757"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0001-706X(97)00056-9"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1136\/bmj.e3866"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1155\/2012\/101895"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1995-7645(13)60126-1"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1007\/s15010-011-0205-6"}],"container-title":["Parasite Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fpim.12151","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/pim.12151","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,1]],"date-time":"2023-10-01T22:10:51Z","timestamp":1696198251000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/pim.12151"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,12,29]]},"references-count":11,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2015,1]]}},"alternative-id":["10.1111\/pim.12151"],"URL":"https:\/\/doi.org\/10.1111\/pim.12151","archive":["Portico"],"relation":{},"ISSN":["0141-9838","1365-3024"],"issn-type":[{"value":"0141-9838","type":"print"},{"value":"1365-3024","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,12,29]]}}}