{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T19:20:33Z","timestamp":1791400833074,"version":"4.3.3"},"reference-count":31,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2015,1,22]],"date-time":"2015-01-22T00:00:00Z","timestamp":1421884800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Grant Agency of Czech Republic","award":["14-25799S"],"award-info":[{"award-number":["14-25799S"]}]},{"name":"Grant Agency of Czech Republic","award":["P302\/12\/2208"],"award-info":[{"award-number":["P302\/12\/2208"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Parasite Immunology"],"published-print":{"date-parts":[[2015,2]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>Type I interferon (<jats:styled-content style=\"fixed-case\">IFN<\/jats:styled-content>), mainly produced by dendritic cells (<jats:styled-content style=\"fixed-case\">DC<\/jats:styled-content>s), is critical in the host defence against tick\u2010transmitted pathogens. Here, we report that salivary cysteine protease inhibitor from the hard tick Ixodes scapularis, sialostatin L2, affects <jats:styled-content style=\"fixed-case\">IFN<\/jats:styled-content>\u2010\u03b2 mediated immune reactions in mouse dendritic cells. Following <jats:styled-content style=\"fixed-case\">IFN<\/jats:styled-content> receptor ligation, the Janus activated kinases\/signal transducer and activator of transcription (<jats:styled-content style=\"fixed-case\">JAK<\/jats:styled-content>\/<jats:styled-content style=\"fixed-case\">STAT<\/jats:styled-content>) pathway is activated. We show that sialostatin L2 attenuates phosphorylation of <jats:styled-content style=\"fixed-case\">STAT<\/jats:styled-content>s in spleen dendritic cells upon addition of recombinant <jats:styled-content style=\"fixed-case\">IFN<\/jats:styled-content>\u2010\u03b2. <jats:styled-content style=\"fixed-case\">LPS<\/jats:styled-content>\u2010stimulated dendritic cells release <jats:styled-content style=\"fixed-case\">IFN<\/jats:styled-content>\u2010\u03b2 which in turn leads to the induction of <jats:styled-content style=\"fixed-case\">IFN<\/jats:styled-content>\u2010stimulated genes (<jats:styled-content style=\"fixed-case\">ISG<\/jats:styled-content>) through <jats:styled-content style=\"fixed-case\">JAK<\/jats:styled-content>\/<jats:styled-content style=\"fixed-case\">STAT<\/jats:styled-content> pathway activation. The induction of two <jats:styled-content style=\"fixed-case\">ISG<\/jats:styled-content>, interferon regulatory factor 7 (<jats:styled-content style=\"fixed-case\">IRF<\/jats:styled-content>\u20107) and <jats:styled-content style=\"fixed-case\">IP<\/jats:styled-content>\u201010, was suppressed by sialostatin L2 in <jats:styled-content style=\"fixed-case\">LPS<\/jats:styled-content>\u2010stimulated dendritic cells. Finally, the interference of sialostatin L2 with <jats:styled-content style=\"fixed-case\">IFN<\/jats:styled-content> action led to the enhanced replication of tick\u2010borne encephalitis virus in <jats:styled-content style=\"fixed-case\">DC<\/jats:styled-content>. In summary, we present here that tick salivary cystatin negatively affects <jats:styled-content style=\"fixed-case\">IFN<\/jats:styled-content>\u2010\u03b2 responses which may consequently increase the pathogen load after transmission via tick saliva.<\/jats:p>","DOI":"10.1111\/pim.12162","type":"journal-article","created":{"date-parts":[[2014,11,19]],"date-time":"2014-11-19T05:43:06Z","timestamp":1416375786000},"page":"70-78","source":"Crossref","is-referenced-by-count":56,"title":["Tick salivary cystatin sialostatin L2 suppresses <scp>IFN<\/scp> responses in mouse dendritic cells"],"prefix":"10.1111","volume":"37","author":[{"given":"J.","family":"Lieskovsk\u00e1","sequence":"first","affiliation":[{"name":"Faculty of Science University of South Bohemia \u010cesk\u00e9 Bud\u011bjovice Czech Republic"},{"name":"Institute of Parasitology Biology Centre of the Academy of Sciences of the Czech Republic \u010cesk\u00e9 Bud\u011bjovice Czech 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