{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T12:34:06Z","timestamp":1788870846998,"version":"build-2803163510"},"reference-count":54,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2015,6,17]],"date-time":"2015-06-17T00:00:00Z","timestamp":1434499200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"The Higher Education Research Promotion and National Research University Project of Thailand, Office of the Higher Education Commission, through the Health Cluster (SHeP-GMS), The National Research Project of Khon Kaen University, Thailand","award":["1165 and PhD54204"],"award-info":[{"award-number":["1165 and PhD54204"]}]},{"name":"The Targeted Research Grant from the Faculty of Medicine, Khon Kaen University, Thailand","award":["TR54101"],"award-info":[{"award-number":["TR54101"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Parasite Immunology"],"published-print":{"date-parts":[[2015,7]]},"abstract":"<jats:title>Summary<\/jats:title>\n                  <jats:p>\n                    Opisthorchiasis secondary to\n                    <jats:italic>\n                      <jats:styled-content style=\"fixed-case\">O<\/jats:styled-content>\n                      pisthorchis viverrini\n                    <\/jats:italic>\n                    infection leads to cholangiocellular carcinoma through chronic inflammation of the bile ducts and possibly inducing autoimmunity. It was hypothesized that plasma autoantibodies directed against self\u2010proteins are biomarkers for opisthorchiasis. Plasma from patients with opisthorchiasis was tested using proteins derived from immortalized cholangiocyte cell lines, and spots reacting with plasma were excised and subjected to\n                    <jats:styled-content style=\"fixed-case\">LC<\/jats:styled-content>\n                    \u2010\n                    <jats:styled-content style=\"fixed-case\">MS<\/jats:styled-content>\n                    \/\n                    <jats:styled-content style=\"fixed-case\">MS<\/jats:styled-content>\n                    . Seven protein spots were recognized by\n                    <jats:styled-content style=\"fixed-case\">I<\/jats:styled-content>\n                    g\n                    <jats:styled-content style=\"fixed-case\">G<\/jats:styled-content>\n                    autoantibodies, and the highest matching scored protein was actin\u2010related protein 3 (\n                    <jats:styled-content style=\"fixed-case\">ARP<\/jats:styled-content>\n                    3). The antibody against\n                    <jats:styled-content style=\"fixed-case\">ARP<\/jats:styled-content>\n                    3 was tested in plasma from 55\n                    <jats:italic>\n                      <jats:styled-content style=\"fixed-case\">O<\/jats:styled-content>\n                      .\u00a0viverrini\n                    <\/jats:italic>\n                    \u2010infected patients, 24 patients with others endemic parasitic infections and 17 healthy controls using\n                    <jats:styled-content style=\"fixed-case\">W<\/jats:styled-content>\n                    estern blot and\n                    <jats:styled-content style=\"fixed-case\">ELISA<\/jats:styled-content>\n                    . Immunoreactivity against recombinant\n                    <jats:styled-content style=\"fixed-case\">ARP<\/jats:styled-content>\n                    3 was significantly more prevalent in opisthorchiasis compared to healthy controls at\n                    <jats:styled-content style=\"fixed-case\">W<\/jats:styled-content>\n                    estern blotting and\n                    <jats:styled-content style=\"fixed-case\">ELISA<\/jats:styled-content>\n                    (\n                    <jats:italic>P\u00a0<\/jats:italic>\n                    &lt;\n                    <jats:italic>\u00a0<\/jats:italic>\n                    0\u00b705). Plasma\n                    <jats:styled-content style=\"fixed-case\">ARP<\/jats:styled-content>\n                    3 autoantibody titres were also higher in opisthorchiasis compared to healthy individuals (\n                    <jats:italic>P\u00a0<\/jats:italic>\n                    &lt;\n                    <jats:italic>\u00a0<\/jats:italic>\n                    0\u00b701) and other parasitic infections including\n                    <jats:italic>\n                      <jats:styled-content style=\"fixed-case\">S<\/jats:styled-content>\n                      trongyloides stercoralis\n                    <\/jats:italic>\n                    (\n                    <jats:italic>P\u00a0<\/jats:italic>\n                    &lt;\n                    <jats:italic>\u00a0<\/jats:italic>\n                    0\u00b7001), echinostome (\n                    <jats:italic>P\u00a0<\/jats:italic>\n                    &lt;\n                    <jats:italic>\u00a0<\/jats:italic>\n                    0\u00b705), hookworms (\n                    <jats:italic>P\u00a0<\/jats:italic>\n                    &lt; 0\u00b7001) and\n                    <jats:italic>\n                      <jats:styled-content style=\"fixed-case\">T<\/jats:styled-content>\n                      aenia\n                    <\/jats:italic>\n                    spp. (\n                    <jats:italic>P\u00a0<\/jats:italic>\n                    &lt;\n                    <jats:italic>\u00a0<\/jats:italic>\n                    0\u00b705). It was further characterized in that the\n                    <jats:styled-content style=\"fixed-case\">ARP<\/jats:styled-content>\n                    3 autoantibody titre had a sensitivity of 78\u00b718% and specificity of 100% for opisthorchiasis. In conclusion, it may be suggested that plasma anti\u2010\n                    <jats:styled-content style=\"fixed-case\">ARP<\/jats:styled-content>\n                    3 might represent a new diagnostic antibody for opisthorchiasis.\n                  <\/jats:p>","DOI":"10.1111\/pim.12188","type":"journal-article","created":{"date-parts":[[2015,3,24]],"date-time":"2015-03-24T16:57:10Z","timestamp":1427216230000},"page":"340-348","source":"Crossref","is-referenced-by-count":8,"title":["Plasma\n                    <scp>I<\/scp>\n                    g\n                    <scp>G<\/scp>\n                    autoantibody against actin\u2010related protein 3 in liver fluke\n                    <i>\n                      <scp>O<\/scp>\n                      pisthorchis viverrini\n                    <\/i>\n                    infection"],"prefix":"10.1111","volume":"37","author":[{"given":"R.","family":"Rucksaken","sequence":"first","affiliation":[{"name":"Department of Parasitology Faculty of Medicine Khon Kaen University Khon Kaen Thailand"},{"name":"Liver Fluke and Cholangiocarcinoma Research Center Faculty of Medicine Khon Kaen University Khon Kaen Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"O.","family":"Haonon","sequence":"additional","affiliation":[{"name":"Department of Parasitology Faculty of Medicine Khon Kaen University Khon Kaen Thailand"},{"name":"Liver Fluke and Cholangiocarcinoma Research Center Faculty of Medicine Khon Kaen University Khon Kaen Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P.","family":"Pinlaor","sequence":"additional","affiliation":[{"name":"Liver Fluke and Cholangiocarcinoma Research Center Faculty of Medicine Khon Kaen University Khon Kaen Thailand"},{"name":"Centre for Research and Development in Medical Diagnostic Laboratory Faculty of Associated Medical Sciences Khon Kaen University Khon Kaen Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C.","family":"Pairojkul","sequence":"additional","affiliation":[{"name":"Liver Fluke and Cholangiocarcinoma Research Center Faculty of Medicine Khon Kaen University Khon Kaen Thailand"},{"name":"Department of Pathology Faculty of Medicine Khon Kaen University Khon Kaen Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S.","family":"Roytrakul","sequence":"additional","affiliation":[{"name":"Proteomics Research Laboratory, Genome Institute National Center for Genetic Engineering and Biotechnology Pathumthani Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P.","family":"Yongvanit","sequence":"additional","affiliation":[{"name":"Liver Fluke and Cholangiocarcinoma Research Center Faculty of Medicine Khon Kaen University Khon Kaen Thailand"},{"name":"Department of Biochemistry Faculty of Medicine Khon Kaen University Khon Kaen Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C.","family":"Selmi","sequence":"additional","affiliation":[{"name":"Division of Rheumatology and Clinical Immunology Humanitas Clinical and Research Center Rozzano Milan Italy"},{"name":"BIOMETRA Department University of Milan Milan Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S.","family":"Pinlaor","sequence":"additional","affiliation":[{"name":"Department of Parasitology Faculty of Medicine Khon Kaen University Khon Kaen Thailand"},{"name":"Liver Fluke and Cholangiocarcinoma Research Center Faculty of Medicine Khon Kaen University Khon Kaen Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2015,6,17]]},"reference":[{"key":"e_1_2_8_2_1","first-page":"341","article-title":"A review of human carcinogens: Opisthorchis viverrini and Clonorchis sinensis","volume":"100","author":"IARC","year":"2012","journal-title":"IARC Monogr 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