{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,17]],"date-time":"2025-09-17T15:01:53Z","timestamp":1758121313095},"reference-count":32,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2015,6,17]],"date-time":"2015-06-17T00:00:00Z","timestamp":1434499200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Parasite Immunology"],"published-print":{"date-parts":[[2015,7]]},"abstract":"Summary<\/jats:title>Isolation of the soluble exogenous antigens (SEA<\/jats:styled-content>gs), its immune response study and proteome profiling is an essential prerequisite for understanding the molecular pathogenesis of Leishmania donovani<\/jats:italic>. The immunostimulatory potential of L.\u00a0donovani <\/jats:italic>SEA<\/jats:styled-content>gs, purified from culture of L.\u00a0donovani<\/jats:italic> clinical isolate, was evaluated for their ability to induce cellular responses in treated\/cured hamsters. SEA<\/jats:styled-content>gs induced significant proliferative responses in lymphocytes (SI<\/jats:styled-content> 5\u00b76\u00a0\u00b1\u00a02\u00b73; P<\/jats:italic>\u00a0<\u00a00\u00b701) isolated from cured hamster. In addition, significant NO<\/jats:styled-content> production in response to SEA<\/jats:styled-content>gs was also noticed in macrophages of hamsters, mouse and human cell lines (J774A\u20101 and THP<\/jats:styled-content>1). Western blot analyses with antibodies against proteophosphoglycan (PPG<\/jats:styled-content>; surface\u2010expressed and secreted molecule) of L<\/jats:italic>.\u00a0donovani<\/jats:italic> revealed that PPG<\/jats:styled-content> molecules are also present in L.\u00a0donovani <\/jats:italic>SEA<\/jats:styled-content>gs. Mass spectrometry (MS<\/jats:styled-content>)\u2010based proteome analysis of 12 protein bands of SEA<\/jats:styled-content>gs through MALDI<\/jats:styled-content>\u2010TOF<\/jats:styled-content>\/TOF<\/jats:styled-content> endorsed the identification of some Th1\u2010stimulatory immunogenic proteins. These immunogenic proteins may offer increased hope for the discovery of new promising vaccine candidates against visceral leishmaniasis (VL<\/jats:styled-content>). The overall results suggest that immunostimulatory molecules are present in the SEA<\/jats:styled-content>gs, which may be further exploited, for developing a subunit vaccine against VL<\/jats:styled-content> a fatal human disease.<\/jats:p>","DOI":"10.1111\/pim.12189","type":"journal-article","created":{"date-parts":[[2015,3,31]],"date-time":"2015-03-31T02:27:30Z","timestamp":1427768850000},"page":"368-375","source":"Crossref","is-referenced-by-count":11,"title":["Immunostimulatory potential and proteome profiling of Leishmania donovani<\/i> soluble exogenous antigens"],"prefix":"10.1111","volume":"37","author":[{"given":"A.","family":"Kumar","sequence":"first","affiliation":[{"name":"Division of Parasitology Central Drug Research Institute (CDRI\u2010CSIR) Lucknow India"},{"name":"Department of Biotechnology National Institute of Technology Raipur Chhattisgarh 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