{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T21:20:04Z","timestamp":1791148804405,"version":"4.1.0"},"reference-count":28,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2015,10,8]],"date-time":"2015-10-08T00:00:00Z","timestamp":1444262400000},"content-version":"vor","delay-in-days":7,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Tunisian Ministry of Research and Technology (MERST)"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Parasite Immunology"],"published-print":{"date-parts":[[2015,10]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>The C57<jats:styled-content style=\"fixed-case\">BL<\/jats:styled-content>\/6 mouse strain is resistant to <jats:italic>Leishmania (L.) major<\/jats:italic> infection and, unlike susceptible <jats:styled-content style=\"fixed-case\">BALB<\/jats:styled-content>\/c, develops small self\u2010healing cutaneous lesions. The specific antibody responses of C57<jats:styled-content style=\"fixed-case\">BL<\/jats:styled-content>\/6 and <jats:styled-content style=\"fixed-case\">BALB<\/jats:styled-content>\/c mice were previously characterized by the predominance of IgG2a (\u2018resistant\u2019 isotype associated with Th1) and IgG1 (\u2018pathogenic\u2019 isotype associated with Th2) antibodies, respectively. In this study, we looked for the presence of antigens able to elicit an exclusive or predominant IgG1 production during the early stages of C57<jats:styled-content style=\"fixed-case\">BL<\/jats:styled-content>\/6 lesion development and checked whether they are recognized or not by <jats:styled-content style=\"fixed-case\">BALB<\/jats:styled-content>\/c mice. We demonstrate first that IgG2a predominance in C57<jats:styled-content style=\"fixed-case\">BL<\/jats:styled-content>\/6 sera occurs only late after infection, whereas in <jats:styled-content style=\"fixed-case\">BALB<\/jats:styled-content>\/c, IgG1 antibodies dominate mostly in the early stages. Interestingly, soon after inoculation of live amastigotes, C57<jats:styled-content style=\"fixed-case\">BL<\/jats:styled-content>\/6 displayed an exclusive IgG1 reactivity against particular <jats:italic>L.\u00a0major<\/jats:italic> antigens but with <jats:styled-content style=\"fixed-case\">MW<\/jats:styled-content>s different from those identified in <jats:styled-content style=\"fixed-case\">BALB<\/jats:styled-content>\/c. Furthermore, mice immunized with killed amastigotes displayed striking differences in their immunodetection profiles, particularly for the IgG1 isotype. Taken together, the observed differences in the specific antibody repertoires between infected mice resulted, at least in part, from immunological events independent from those triggered by the replicating parasite, and bring new insights into the selection of future vaccine candidates.<\/jats:p>","DOI":"10.1111\/pim.12223","type":"journal-article","created":{"date-parts":[[2015,8,24]],"date-time":"2015-08-24T09:48:12Z","timestamp":1440409692000},"page":"544-552","source":"Crossref","is-referenced-by-count":7,"title":["Identification of immunodominant <i>Leishmania major<\/i> antigenic markers of the early C57<scp>BL<\/scp>\/6 and <scp>BALB<\/scp>\/c mice infection stages"],"prefix":"10.1111","volume":"37","author":[{"given":"A.","family":"Sassi","sequence":"first","affiliation":[{"name":"Laboratory of Immunopathology Vaccinology and Molecular Genetics Institut Pasteur of Tunis University Tunis\u2010El Manar Tunis\u2010Belvedere Tunisia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"O.","family":"Kaak","sequence":"additional","affiliation":[{"name":"Laboratory of Immunopathology Vaccinology and Molecular Genetics Institut Pasteur of Tunis University Tunis\u2010El Manar Tunis\u2010Belvedere Tunisia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.","family":"Ben Ammar Elgaied","sequence":"additional","affiliation":[{"name":"Laboratory of Genetics, Immunology and Human Pathologies Faculty of Sciences University Tunis\u2010El Manar Tunis\u2010Belvedere 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