{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,8]],"date-time":"2026-04-08T00:17:21Z","timestamp":1775607441348,"version":"3.50.1"},"reference-count":48,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2015,10,26]],"date-time":"2015-10-26T00:00:00Z","timestamp":1445817600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Programa Estudantes-Conv\u00eanio de P\u00f3s-Gradua\u00e7\u00e3o (PEC-PG) - CAPES\/CNPq - Brazil"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Parasite Immunology"],"published-print":{"date-parts":[[2015,11]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>Serodiagnosis of human toxocariasis is difficult in tropical areas where other helminthiasis are endemic. Many studies have shown that glycans from helminths may be the responsible for cross\u2010reactions in the immunoassays. In this study, we have evaluated the deglycosylation of the <jats:italic>Toxocara canis<\/jats:italic> excretory\u2013secretory (<jats:styled-content style=\"fixed-case\">TES<\/jats:styled-content>) antigens for the detection of IgG antibodies using a panel of 228 serum samples (58 patients with toxocariasis, 75 patients with other helminth infections and 95 healthy individuals) by <jats:styled-content style=\"fixed-case\">ELISA<\/jats:styled-content> and Western blot assays. Our results showed that the deglycosylation of <jats:styled-content style=\"fixed-case\">TES<\/jats:styled-content> antigens resulted in a single fraction of 26 <jats:styled-content style=\"fixed-case\">kD<\/jats:styled-content>a (<jats:styled-content style=\"fixed-case\">dTES<\/jats:styled-content>) and was able to detect IgG antibodies with a sensitivity and specificity of 100% in both above\u2010mentioned assays. The rate of cross\u2010reactions, observed in <jats:styled-content style=\"fixed-case\">ELISA<\/jats:styled-content> with <jats:styled-content style=\"fixed-case\">TES<\/jats:styled-content> (13\u00b73%), was significantly reduced (5\u00b73%) when the <jats:styled-content style=\"fixed-case\">dTES<\/jats:styled-content> antigens were used. Likewise, the cross\u2010reactivity observed with the fractions of 32, 55 and 70 <jats:styled-content style=\"fixed-case\">kD<\/jats:styled-content>a of the <jats:styled-content style=\"fixed-case\">TES<\/jats:styled-content> antigens was totally eliminated when the <jats:styled-content style=\"fixed-case\">dTES<\/jats:styled-content> were used in the Western blot. All these results showed that the deglycosylation of the <jats:styled-content style=\"fixed-case\">TES<\/jats:styled-content> antigens really improves the specificity of the serodiagnosis of human toxocariasis in endemic areas for helminth infections.<\/jats:p>","DOI":"10.1111\/pim.12248","type":"journal-article","created":{"date-parts":[[2015,8,28]],"date-time":"2015-08-28T07:21:38Z","timestamp":1440746498000},"page":"557-567","source":"Crossref","is-referenced-by-count":20,"title":["Deglycosylation of <i>Toxocara<\/i> excretory\u2013secretory antigens improves the specificity of the serodiagnosis for human toxocariasis"],"prefix":"10.1111","volume":"37","author":[{"given":"W.H.","family":"Rold\u00e1n","sequence":"first","affiliation":[{"name":"Laborat\u00f3rio de Soroepidemiologia e Imunobiologia Instituto de Medicina Tropical de S\u00e3o Paulo Universidade de S\u00e3o Paulo S\u00e3o Paulo SP Brazil"},{"name":"Programa de P\u00f3s\u2010gradua\u00e7\u00e3o em Doen\u00e7as Infecciosas e Parasit\u00e1rias Faculdade de Medicina Universidade de S\u00e3o Paulo S\u00e3o Paulo SP Brazil"},{"name":"Departamento de Microbiologia M\u00e9dica Facultad de Medicina Universidad Nacional Mayor de San Marcos Lima Peru"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G. 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