{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T23:47:21Z","timestamp":1790380041477,"version":"4.1.0"},"reference-count":65,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2015,12,23]],"date-time":"2015-12-23T00:00:00Z","timestamp":1450828800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100002322","name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Parasite Immunology"],"published-print":{"date-parts":[[2016,1]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>Cutaneous leishmaniasis usually presents therapeutic resistance to antimonials, and the existing therapies for leishmaniasis have many adverse effects and toxicity. Natural products may be regarded as possible candidates for alternative leishmaniasis treatment. The plant <jats:italic>Tetradenia riparia<\/jats:italic> has shown promise for the treatment of infectious diseases in folk medicine. We evaluated the antileishmanial activity of an essential oil from <jats:italic>T.\u00a0riparia<\/jats:italic> (Tr<jats:styled-content style=\"fixed-case\">EO<\/jats:styled-content>) and the modulatory effects of Tr<jats:styled-content style=\"fixed-case\">EO<\/jats:styled-content> on cytokine modulation by peritoneal fluid cells that were infected with <jats:italic>L.\u00a0(L.) amazonensis<\/jats:italic>. Peritoneal fluid cells were infected with <jats:italic>Leishmania<\/jats:italic> and incubated with Tr<jats:styled-content style=\"fixed-case\">EO<\/jats:styled-content> (30\u00a0ng\/mL) for 3, 6, and 24\u00a0h. Cytokines were screened using semi\u2010quantitative reverse\u2010transcription polymerase chain reaction (<jats:styled-content style=\"fixed-case\">RT<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content>) and flow cytometry. Antileishmanial activity was evaluated at 24\u00a0h by microscopic counting and quantitative <jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content> (<jats:styled-content style=\"fixed-case\">qPCR<\/jats:styled-content>). Tr<jats:styled-content style=\"fixed-case\">EO<\/jats:styled-content> treatment induced the death of 50% of <jats:italic>Leishmania<\/jats:italic> amastigotes (indicated by microscopic counting) and 91% of the parasite load (indicated by <jats:styled-content style=\"fixed-case\">qPCR<\/jats:styled-content>). Tr<jats:styled-content style=\"fixed-case\">EO<\/jats:styled-content> inhibited some of the most critical cytokines for parasite growth and the establishment of infection, including granulocyte\u2010macrophage colony\u2010stimulating factor, interleukin\u20104 (<jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u20104), <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u201010, and tumour necrosis factor. The parasite inhibited interferon\u2010\u03b3 and <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u201012, and Tr<jats:styled-content style=\"fixed-case\">EO<\/jats:styled-content> blocked this inhibition, indicating that these cytokines are critical for activating mechanisms associated with the death and elimination of the parasite. These results suggest that Tr<jats:styled-content style=\"fixed-case\">EO<\/jats:styled-content> may be an alternative leishmaniasis therapy when considering its antileishmanial and immunomodulatory activity.<\/jats:p>","DOI":"10.1111\/pim.12297","type":"journal-article","created":{"date-parts":[[2015,11,28]],"date-time":"2015-11-28T15:12:43Z","timestamp":1448723563000},"page":"64-77","source":"Crossref","is-referenced-by-count":21,"title":["Antileishmanial and immunomodulatory effects of the essential oil from <i>Tetradenia riparia<\/i> (Hochstetter) Codd"],"prefix":"10.1111","volume":"38","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5764-9511","authenticated-orcid":false,"given":"Izabel Galhardo","family":"Demarchi","sequence":"first","affiliation":[{"name":"Departamento de An\u00e1lises Cl\u00ednicas e Biomedicina Laborat\u00f3rio de Imunologia Cl\u00ednica Universidade Estadual de Maring\u00e1 Maring\u00e1 Paran\u00e1 Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mariana de Souza","family":"Terron","sequence":"additional","affiliation":[{"name":"Departamento de An\u00e1lises Cl\u00ednicas e Biomedicina Laborat\u00f3rio de Imunologia Cl\u00ednica Universidade Estadual de Maring\u00e1 Maring\u00e1 Paran\u00e1 Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mateus Vailant","family":"Thomazella","sequence":"additional","affiliation":[{"name":"Departamento de An\u00e1lises Cl\u00ednicas e Biomedicina Laborat\u00f3rio de Imunologia Cl\u00ednica Universidade Estadual de Maring\u00e1 Maring\u00e1 Paran\u00e1 Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Camila Alves","family":"Mota","sequence":"additional","affiliation":[{"name":"Departamento de An\u00e1lises Cl\u00ednicas e Biomedicina Laborat\u00f3rio de Imunologia Cl\u00ednica Universidade Estadual de Maring\u00e1 Maring\u00e1 Paran\u00e1 Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zilda Cristiani","family":"Gazim","sequence":"additional","affiliation":[{"name":"Departamento de Farm\u00e1cia Laborat\u00f3rio de Qu\u00edmica de Produtos Naturais da Universidade Paranaense Umuarama Paran\u00e1 Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Di\u00f3genez Apar\u00edcio Garcia","family":"Cortez","sequence":"additional","affiliation":[{"name":"Departamento de Farm\u00e1cia e Farmacologia Universidade Estadual de Maring\u00e1 Maring\u00e1 Paran\u00e1 Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sandra Mara Alessi","family":"Aristides","sequence":"additional","affiliation":[{"name":"Departamento de An\u00e1lises Cl\u00ednicas e Biomedicina Laborat\u00f3rio de Imunologia Cl\u00ednica Universidade Estadual de Maring\u00e1 Maring\u00e1 Paran\u00e1 Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tha\u00eds Gomes Verzignassi","family":"Silveira","sequence":"additional","affiliation":[{"name":"Departamento de An\u00e1lises Cl\u00ednicas e Biomedicina Laborat\u00f3rio de Imunologia Cl\u00ednica Universidade Estadual de Maring\u00e1 Maring\u00e1 Paran\u00e1 Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria Valdrinez Campana","family":"Lonardoni","sequence":"additional","affiliation":[{"name":"Departamento de An\u00e1lises Cl\u00ednicas e Biomedicina Laborat\u00f3rio de Imunologia Cl\u00ednica Universidade Estadual de Maring\u00e1 Maring\u00e1 Paran\u00e1 Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2015,12,23]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-3024.2009.01116.x"},{"key":"e_1_2_6_3_1","unstructured":"WHO (World Health Organization).Control of the Leishmaniasis: report of a meeting of the WHO Expert Committee on the Control of Leihmaniases. Geneva Switzerland WHO technical report series 2010."},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/pim.12100"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1000541"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1517\/14656566.2013.755515"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkt262"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1007\/s40265-013-0132-1"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.actatropica.2011.02.007"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2014.00193"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.2174\/157489111794407868"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bmc.2013.11.048"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules15085509"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.exppara.2011.06.011"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1080\/08923970802135187"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1201\/9780203013717"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-8741(99)00035-5"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/0378-8741(88)90156-0"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2011.03.072"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2012.08.038"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1055\/s-2006-957672"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2011.11.034"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/0378-8741(86)90115-7"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.exppara.2015.06.014"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cyto.2013.12.016"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.1002\/ffj.3284"},{"key":"e_1_2_6_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.phytochem.2004.08.035"},{"key":"e_1_2_6_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.phymed.2009.01.011"},{"key":"e_1_2_6_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mimet.2003.08.013"},{"key":"e_1_2_6_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bmc.2005.07.012"},{"key":"e_1_2_6_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tox.2003.12.013"},{"key":"e_1_2_6_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajpath.2011.03.010"},{"key":"e_1_2_6_33_1","doi-asserted-by":"publisher","DOI":"10.1590\/S0074-02762011000500010"},{"key":"e_1_2_6_34_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00018-010-0457-9"},{"key":"e_1_2_6_35_1","doi-asserted-by":"publisher","DOI":"10.1590\/S0074-02762010000100018"},{"key":"e_1_2_6_36_1","doi-asserted-by":"publisher","DOI":"10.1645\/GE-264R1"},{"key":"e_1_2_6_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.exppara.2012.03.021"},{"key":"e_1_2_6_38_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules19010514"},{"key":"e_1_2_6_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2010.10.053"},{"key":"e_1_2_6_40_1","first-page":"27","article-title":"Visceral leishmaniasis: experimental models for drug discovery","volume":"133","author":"Nishi GS","year":"2011","journal-title":"Indian J Med Res"},{"key":"e_1_2_6_41_1","doi-asserted-by":"publisher","DOI":"10.2217\/imt.14.77"},{"key":"e_1_2_6_42_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.coi.2014.12.001"},{"key":"e_1_2_6_43_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-695X.2007.00311.x"},{"key":"e_1_2_6_44_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.phymed.2011.03.004"},{"key":"e_1_2_6_45_1","doi-asserted-by":"publisher","DOI":"10.1002\/eji.200323825"},{"key":"e_1_2_6_46_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.189.4.741"},{"key":"e_1_2_6_47_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.immunol.19.1.423"},{"key":"e_1_2_6_48_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.gt.3302240"},{"key":"e_1_2_6_49_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.68.5.2449-2456.2000"},{"key":"e_1_2_6_50_1","doi-asserted-by":"publisher","DOI":"10.1155\/2014\/481750"},{"key":"e_1_2_6_51_1","doi-asserted-by":"publisher","DOI":"10.1002\/eji.200636012"},{"key":"e_1_2_6_52_1","first-page":"66","article-title":"Impact of tumor necrosis factor receptor p55 deficiency in susceptibility of C57BL\/6 mice to infection with Leishmania (Leishmania) amazonensis","volume":"1684","author":"Cargnelutti DE","year":"2014","journal-title":"J Microbiol Immunol Infect"},{"key":"e_1_2_6_53_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1303043"},{"key":"e_1_2_6_54_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm.3221"},{"key":"e_1_2_6_55_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature08900"},{"key":"e_1_2_6_56_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2005.09.015"},{"key":"e_1_2_6_57_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0003331"},{"key":"e_1_2_6_58_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0901206106"},{"key":"e_1_2_6_59_1","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.00383-13"},{"key":"e_1_2_6_60_1","doi-asserted-by":"crossref","first-page":"3523","DOI":"10.1128\/iai.60.9.3523-3527.1992","article-title":"Granulocyte\u2010macrophage colony\u2010stimulating factor increases the infectivity of Leishmania amazonensis by protecting promastigotes from heat\u2010induced death","volume":"60","author":"Barcinski MA","year":"1992","journal-title":"Infect Immun"},{"key":"e_1_2_6_61_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1550-7408.1990.tb01157.x"},{"key":"e_1_2_6_62_1","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/162.1.224"},{"key":"e_1_2_6_63_1","doi-asserted-by":"publisher","DOI":"10.1002\/jlb.51.3.220"},{"key":"e_1_2_6_64_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.phymed.2014.07.004"},{"key":"e_1_2_6_65_1","doi-asserted-by":"publisher","DOI":"10.15517\/rbt.v62i2.9396"},{"key":"e_1_2_6_66_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules17043989"}],"container-title":["Parasite Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fpim.12297","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/pim.12297","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,13]],"date-time":"2023-09-13T10:24:19Z","timestamp":1694600659000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/pim.12297"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,12,23]]},"references-count":65,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2016,1]]}},"alternative-id":["10.1111\/pim.12297"],"URL":"https:\/\/doi.org\/10.1111\/pim.12297","archive":["Portico"],"relation":{},"ISSN":["0141-9838","1365-3024"],"issn-type":[{"value":"0141-9838","type":"print"},{"value":"1365-3024","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,12,23]]}}}