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The horizontal transmission of <jats:italic>Toxoplasma<\/jats:italic> is even more common than congenital. An effective vaccine strategy brings the prospect of improving <jats:italic>Toxoplasma<\/jats:italic> disease control. Rhoptry protein 5 (<jats:styled-content style=\"fixed-case\">ROP<\/jats:styled-content>5) and <jats:styled-content style=\"fixed-case\">ROP<\/jats:styled-content>7 are potential stimulators of humoral and cellular immune responses. In this study, we constructed a multi\u2010antigenic <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> vaccine expressing <jats:styled-content style=\"fixed-case\">ROP<\/jats:styled-content>5 and <jats:styled-content style=\"fixed-case\">ROP<\/jats:styled-content>7 of <jats:italic>T.\u00a0gondii<\/jats:italic> and compared the protective efficacy to single\u2010gene vaccines and control groups. <jats:styled-content style=\"fixed-case\">BALB<\/jats:styled-content>\/c mice were immunized intramuscularly three times. The levels of IgG antibodies and cytokines in mice immunized with the multi\u2010antigenic <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> vaccine (<jats:styled-content style=\"fixed-case\">pROP<\/jats:styled-content>5\/<jats:styled-content style=\"fixed-case\">ROP<\/jats:styled-content>7) were significantly higher than those in the control mice. Mice vaccinated with <jats:styled-content style=\"fixed-case\">pROP<\/jats:styled-content>5\/<jats:styled-content style=\"fixed-case\">ROP<\/jats:styled-content>7 showed a longer survival time (16\u00a0days) than single\u2010gene\u2010immunized mice (11 and 12\u00a0days, respectively) or control mice (8\u00a0days) after a challenge with 1\u00a0\u00d7\u00a010<jats:sup>4<\/jats:sup> tachyzoites of <jats:styled-content style=\"fixed-case\">RH<\/jats:styled-content> strain of <jats:italic>T.\u00a0<\/jats:italic>gondii. Furthermore, after intragastric infection with 20 cysts of <jats:styled-content style=\"fixed-case\">PRU<\/jats:styled-content> strain of <jats:italic>T.\u00a0gondii<\/jats:italic>, the number of brain cysts in mice immunized with <jats:styled-content style=\"fixed-case\">pROP<\/jats:styled-content>5\/<jats:styled-content style=\"fixed-case\">ROP<\/jats:styled-content>7 was only 25% of the number in control mice. Our results showed that a <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> vaccine encoding <jats:styled-content style=\"fixed-case\">ROP<\/jats:styled-content>5 and <jats:styled-content style=\"fixed-case\">ROP<\/jats:styled-content>7 significantly enhanced protection against <jats:italic>T.\u00a0gondii<\/jats:italic> challenge.<\/jats:p>","DOI":"10.1111\/pim.12306","type":"journal-article","created":{"date-parts":[[2016,1,23]],"date-time":"2016-01-23T14:22:40Z","timestamp":1453558960000},"page":"209-217","source":"Crossref","is-referenced-by-count":21,"title":["Evaluation of immune responses induced by rhoptry protein 5 and rhoptry protein 7 <scp>DNA<\/scp> vaccines against <i>Toxoplasma gondii<\/i>"],"prefix":"10.1111","volume":"38","author":[{"given":"L.","family":"Wang","sequence":"first","affiliation":[{"name":"Department of Parasitology Shandong University School of Medicine Jinan Shandong China"},{"name":"Department of Ji Nan Children's Hospital 250022 Jinan 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China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H.","family":"Zhou","sequence":"additional","affiliation":[{"name":"Department of Parasitology Shandong University School of Medicine Jinan Shandong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H.","family":"Cong","sequence":"additional","affiliation":[{"name":"Department of Parasitology Shandong University School of Medicine Jinan Shandong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S.","family":"He","sequence":"additional","affiliation":[{"name":"Department of Parasitology Shandong University School of Medicine Jinan Shandong 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