{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,27]],"date-time":"2026-01-27T13:14:18Z","timestamp":1769519658664,"version":"3.49.0"},"reference-count":34,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2016,3,30]],"date-time":"2016-03-30T00:00:00Z","timestamp":1459296000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100003725","name":"National Research Foundation of Korea","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004085","name":"Ministry of Education, Science and Technology","doi-asserted-by":"publisher","award":["2011\u20100012374"],"award-info":[{"award-number":["2011\u20100012374"]}],"id":[{"id":"10.13039\/501100004085","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Parasite Immunology"],"published-print":{"date-parts":[[2016,4]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>While <jats:italic>Trichomonas vaginalis<\/jats:italic>, a cause of sexually transmitted infection, is known as a surface\u2010dwelling protozoa, trichomonads have been detected in prostatic tissue from benign prostatic hyperplasia and prostatitis by immunoperoxidase assay or PCR. However, the immune response of prostate stromal cells infected with <jats:italic>T.\u00a0vaginalis<\/jats:italic> has not been investigated. Our objective was to investigate whether <jats:italic>T.\u00a0vaginalis<\/jats:italic> could induce an inflammatory response in prostate stromal cells. Incubation of a human prostate stromal myofibroblast cells (WPMY\u20101) with live <jats:italic>T.\u00a0vaginalis<\/jats:italic> T016 increased expression of the inflammatory chemokines CXCL8 and CCL2. In addition, TLR4, ROS, MAPK and NF\u2010\u03baB expression increased, while inhibitors of TLR4, ROS, MAPKs and NF\u2010\u03baB reduced CXCL8 and CCL2 production. Medium conditioned by incubation of WPMY\u20101 cells with <jats:italic>T.\u00a0vaginalis<\/jats:italic> stimulated the migration of human neutrophils and monocytes (THP\u20101 cells). We conclude that <jats:italic>T.\u00a0vaginalis<\/jats:italic> increases CXCL8 and CCL2 production by human prostate stromal cells by activating TLR4, ROS, MAPKs and NF\u2010\u03baB, and this in turn attracts neutrophils and monocytes and leads to an inflammatory response. This study is the first attempt to demonstrate an inflammatory reaction in prostate stromal cells caused by <jats:italic>T.\u00a0vaginalis<\/jats:italic>.<\/jats:p>","DOI":"10.1111\/pim.12308","type":"journal-article","created":{"date-parts":[[2016,2,1]],"date-time":"2016-02-01T08:58:21Z","timestamp":1454317101000},"page":"218-227","source":"Crossref","is-referenced-by-count":20,"title":["Inflammatory response of a prostate stromal cell line induced by <i>Trichomonas vaginalis<\/i>"],"prefix":"10.1111","volume":"38","author":[{"given":"S. J.","family":"Im","sequence":"first","affiliation":[{"name":"Department of Environmental Biology and Medical Parasitology Hanyang University College of Medicine Seongdong\u2010gu Seoul Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"I. H.","family":"Han","sequence":"additional","affiliation":[{"name":"Department of Environmental Biology and Medical Parasitology Hanyang University College of Medicine Seongdong\u2010gu Seoul Korea"},{"name":"Department of Biomedical Science Hanyang University Graduate School of Biomedical Science and Engineering Seongdong\u2010gu Seoul Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J. H.","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Environmental Biology and Medical Parasitology Hanyang University College of Medicine Seongdong\u2010gu Seoul Korea"},{"name":"Department of Biomedical Science Hanyang University Graduate School of Biomedical Science and Engineering Seongdong\u2010gu Seoul Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"N. Y.","family":"Gu","sequence":"additional","affiliation":[{"name":"Department of Environmental Biology and Medical Parasitology Hanyang University College of Medicine Seongdong\u2010gu Seoul Korea"},{"name":"Department of Biomedical Science Hanyang University Graduate School of Biomedical Science and Engineering Seongdong\u2010gu Seoul Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M. 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