{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T12:55:12Z","timestamp":1791377712345,"version":"4.3.2"},"reference-count":36,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2016,3,30]],"date-time":"2016-03-30T00:00:00Z","timestamp":1459296000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Parasite Immunology"],"published-print":{"date-parts":[[2016,4]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>Serodiagnosis of human toxocariasis is based on the detection of specific IgG antibodies by the enzyme\u2010linked immunosorbent assay (<jats:styled-content style=\"fixed-case\">ELISA<\/jats:styled-content>) using <jats:italic>Toxocara<\/jats:italic> larvae excretory\u2013secretory (<jats:styled-content style=\"fixed-case\">TES<\/jats:styled-content>) antigens, but its production is a laborious and time\u2010consuming process being also limited by the availability of adult females of <jats:italic>T.\u00a0canis<\/jats:italic> as source for ova to obtain larvae. Chemical synthesis of the di\u2010<jats:italic>O<\/jats:italic>\u2010methylated (DiM) glycan structure found in the <jats:styled-content style=\"fixed-case\">TES<\/jats:styled-content> antigens has provided material for studying the antibody reactivity in a range of mammalian hosts, showing reactivity with human IgM and IgG. In this study, we have evaluated the performance of the DiM glycan against a panel of sera including patients with toxocariasis (<jats:italic>n<\/jats:italic>\u00a0=\u00a060), patients with other helminth infections (<jats:italic>n<\/jats:italic>\u00a0=\u00a075) and healthy individuals (<jats:italic>n<\/jats:italic>\u00a0=\u00a094), showing that DiM is able to detect IgG antibodies with a sensitivity and specificity of 91\u00b77% and 94\u00b77%, respectively, with a very good agreement with the <jats:styled-content style=\"fixed-case\">TES<\/jats:styled-content> antigens (kappa = 0\u00b7825). However, cross\u2010reactivity was observed in some sera from patients with ascariasis, hymenolepiasis and fascioliasis. These results show that the DiM glycan could be a promising antigenic tool for the serodiagnosis of human toxocariasis.<\/jats:p>","DOI":"10.1111\/pim.12311","type":"journal-article","created":{"date-parts":[[2016,2,20]],"date-time":"2016-02-20T20:41:56Z","timestamp":1456000916000},"page":"236-243","source":"Crossref","is-referenced-by-count":9,"title":["Evaluation of a di\u2010<i>O<\/i>\u2010methylated glycan as a potential antigenic target for the serodiagnosis of human toxocariasis"],"prefix":"10.1111","volume":"38","author":[{"given":"G. R.","family":"Elefant","sequence":"first","affiliation":[{"name":"Laborat\u00f3rio de Soroepidemiologia e Imunobiologia do Instituto de Medicina Tropical de S\u00e3o Paulo Universidade de S\u00e3o Paulo S\u00e3o Paulo Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"W. 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