{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,9]],"date-time":"2026-06-09T12:40:25Z","timestamp":1781008825211,"version":"3.54.1"},"reference-count":55,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2016,6,20]],"date-time":"2016-06-20T00:00:00Z","timestamp":1466380800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100006109","name":"Vedeck\u00e1 Grantov\u00e1 Agent\u00fara M\u0160VVa\u0160 SR a SAV","doi-asserted-by":"publisher","award":["2\/0150\/13"],"award-info":[{"award-number":["2\/0150\/13"]}],"id":[{"id":"10.13039\/501100006109","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Parasite Immunology"],"published-print":{"date-parts":[[2016,7]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>Cestode\u2010mediated diseases hold the interesting feature of persisting metacestode larvae dwelling within the host tissues, in the midst of the immune response. Excretory\u2013secretory (<jats:styled-content style=\"fixed-case\">ES<\/jats:styled-content>) products of the metacestode larval stage modulate the host immune response and modify the outcome of the disease. Therefore, isolation and analysis of axenic metacestode <jats:styled-content style=\"fixed-case\">ES<\/jats:styled-content> products are crucial to study their properties. Here, we report the development of a system for long\u2010term <jats:italic>in\u00a0vitro<\/jats:italic> cultivation of the metacestode of the parasitic cestode <jats:italic>Mesocestoides corti<\/jats:italic> (syn. <jats:italic>Mesocestoides vogae<\/jats:italic>). Although feeder cells and host serum supported the early growth of the parasite, long\u2010term survival was not dependent on host serum or host\u2010derived factors enabling the collection of parasite released products in serum\u2010free medium. Functionally, these axenic <jats:styled-content style=\"fixed-case\">ES<\/jats:styled-content> products recapitulated <jats:italic>M.\u00a0corti<\/jats:italic> tetrathyridia's ability to inhibit <jats:styled-content style=\"fixed-case\">LPS<\/jats:styled-content>\u2010driven <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u201012p70 secretion by dendritic cells. Thus, our new axenic culture system will simplify the identification and characterization of <jats:italic>M.\u00a0corti<\/jats:italic>\u2010derived immunomodulatory factors that will indirectly enable the identification and characterization of corresponding factors in the metacestode larvae of medically relevant cestodes such as <jats:italic>Echinococcus multilocularis<\/jats:italic> that are not yet amenable to serum\u2010free cultivation.<\/jats:p>","DOI":"10.1111\/pim.12327","type":"journal-article","created":{"date-parts":[[2016,4,27]],"date-time":"2016-04-27T23:41:04Z","timestamp":1461800464000},"page":"403-413","source":"Crossref","is-referenced-by-count":13,"title":["<i>In vitro<\/i> culture of <i>Mesocestoides corti<\/i> metacestodes and isolation of immunomodulatory excretory\u2013secretory products"],"prefix":"10.1111","volume":"38","author":[{"given":"E.","family":"Vendelova","sequence":"first","affiliation":[{"name":"Institute of Parasitology of the Slovak Academy of Sciences Ko\u0161ice Slovak Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G.","family":"Hr\u010dkov\u00e1","sequence":"additional","affiliation":[{"name":"Institute of Parasitology of the Slovak Academy of Sciences Ko\u0161ice Slovak Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M. 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