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The whole body (<jats:styled-content style=\"fixed-case\">WB<\/jats:styled-content>) extracts of <jats:italic>P. gracile<\/jats:italic> were fractionated by gel filtration chromatography in a Sephadex G\u2010200 column. It was found that the <jats:styled-content style=\"fixed-case\">WB<\/jats:styled-content> extract fractions, F1\u2013F3 were highly antigenic, F5 was moderately antigenic and F4 was poorly antigenic. For <jats:styled-content style=\"fixed-case\">SDS<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">PAGE<\/jats:styled-content> and immunoblotting, the antigenic molecules of <jats:styled-content style=\"fixed-case\">WB<\/jats:styled-content> extract and all five fractions were mostly at molecular weights (<jats:styled-content style=\"fixed-case\">MW<\/jats:styled-content>) ranging from 12 to 150 <jats:styled-content style=\"fixed-case\">kD<\/jats:styled-content>a. One antigenic protein of 16 <jats:styled-content style=\"fixed-case\">kD<\/jats:styled-content>a detected in <jats:styled-content style=\"fixed-case\">WB<\/jats:styled-content> extract and F1\u2013F3 was found to give a consistent reaction with sera from infected cattle. The antigenicity of the purified 16 <jats:styled-content style=\"fixed-case\">kD<\/jats:styled-content>a protein was confirmed by immunoblotting and indirect <jats:styled-content style=\"fixed-case\">ELISA<\/jats:styled-content> using a pool of sera and individual serum samples from infected cattle (at 1 : 78 125 dilution) and hyperimmunized rabbit (at 1 : 390 625 dilution). This finding suggests that the 16 <jats:styled-content style=\"fixed-case\">kD<\/jats:styled-content>a protein may be a potential antigen for the immunodiagnosis of cattle paramphistomosis caused by <jats:italic>P. gracile<\/jats:italic>.<\/jats:p>","DOI":"10.1111\/pim.12330","type":"journal-article","created":{"date-parts":[[2016,5,2]],"date-time":"2016-05-02T16:42:35Z","timestamp":1462207355000},"page":"431-438","source":"Crossref","is-referenced-by-count":11,"title":["Antigenic profile, isolation and characterization of whole body extract of <i>Paramphistomum gracile<\/i>"],"prefix":"10.1111","volume":"38","author":[{"given":"P.","family":"Anuracpreeda","sequence":"first","affiliation":[{"name":"Division of Agricultural Science Mahidol University Saiyok Kanchanaburi Thailand"},{"name":"Department of Anatomy Faculty of Science Mahidol University Bangkok 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