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In the early stages, the host organism responds with an inflammatory infiltrate composed mostly of neutrophils. It has been reported that these immune cells, activated by E. histolytica, exert a protective role by releasing proteolytic enzymes and generating reactive oxygen\/nitrogen species (<jats:styled-content style=\"fixed-case\">ROS<\/jats:styled-content>\/<jats:styled-content style=\"fixed-case\">RNS<\/jats:styled-content>) and antimicrobial peptides. It is now known that neutrophils also produce neutrophil extracellular traps (<jats:styled-content style=\"fixed-case\">NET<\/jats:styled-content>s), which are able to damage and kill pathogens. Studies have shown that intracellular protozoan pathogens, including Toxoplasma gondi, Plasmodium falciparum and Leishmania spp, induce neutrophils to release <jats:styled-content style=\"fixed-case\">NET<\/jats:styled-content>s and are damaged by them. However, the action of this mechanism has not been explored in relation to E. histolytica trophozoites. Through scanning electron, epifluorescence microscopy and viability assays, we show for first time that during in vitro interaction with E. histolytica trophozoites, human neutrophils released <jats:styled-content style=\"fixed-case\">NET<\/jats:styled-content>s that covered amoebas and reduced amoebic viability. These <jats:styled-content style=\"fixed-case\">NET<\/jats:styled-content>s presented histones, myeloperoxidase and decondensed chromatin. The results suggest that <jats:styled-content style=\"fixed-case\">NET<\/jats:styled-content>s participate in the elimination of the parasite.<\/jats:p>","DOI":"10.1111\/pim.12332","type":"journal-article","created":{"date-parts":[[2016,5,3]],"date-time":"2016-05-03T12:20:47Z","timestamp":1462278047000},"page":"503-509","source":"Crossref","is-referenced-by-count":26,"title":["<i>Entamoeba histolytica<\/i> induces human neutrophils to form <scp>NET<\/scp>s"],"prefix":"10.1111","volume":"38","author":[{"given":"J.","family":"Ventura\u2010Juarez","sequence":"first","affiliation":[{"name":"Centro de Ciencias B\u00e1sicas Universidad Aut\u00f3noma de Aguascalientes Ags M\u00e9xico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"MR.","family":"Campos\u2010Esparza","sequence":"additional","affiliation":[{"name":"Centro de Ciencias B\u00e1sicas Universidad Aut\u00f3noma de Aguascalientes Ags M\u00e9xico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.","family":"Pacheco\u2010Yepez","sequence":"additional","affiliation":[{"name":"Secci\u00f3n de Estudios de Posgrado e Investigaci\u00f3n Escuela Superior de Medicina Instituto Polit\u00e9cnico Nacional Ciudad de M\u00e9xico M\u00e9xico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J. 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