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The large extracellular loop of Schistosoma mansoni<\/jats:italic> tetraspanin\u20102, Sm\u2010TSP<\/jats:styled-content>\u20102, when fused to a thioredoxin partner and formulated with Freund's adjuvants, has been shown to be an efficacious vaccine against murine schistosomiasis. It is well recognized that CD<\/jats:styled-content>4+<\/jats:sup> T\u2010cell\u2010dependent immunity might play an important role against schistosomes; however, the contribution of CD<\/jats:styled-content>8+<\/jats:sup> T cells against multicellular pathogen is still uncertain. The exogenous protein\u2010pulsed dendritic cells (DCs) can easily activate CD<\/jats:styled-content>4+<\/jats:sup> T cells response, while CD<\/jats:styled-content>8+<\/jats:sup> T cells response was relatively difficult to be induced. In this study, we evaluated the immunogenicity of TSP<\/jats:styled-content>2HD<\/jats:styled-content> antigen (hydrophilic domain of the S. japonicum<\/jats:italic> tetraspanin\u20102) and TAT<\/jats:styled-content> (the protein transduction domain of HIV<\/jats:styled-content>\u20101)\u2010coupled TSP<\/jats:styled-content>2HD<\/jats:styled-content> protein. As TAT<\/jats:styled-content>\u2010fused protein could promote major histocompatibility complex class I\u2010dependent antigen presentation in vitro<\/jats:italic>, TAT<\/jats:styled-content>\u2010TSP<\/jats:styled-content>2HD<\/jats:styled-content>\u2010pulsed DC<\/jats:styled-content>s induced stronger proliferation of schistosome\u2010specific CD<\/jats:styled-content>8+<\/jats:sup> T cells compared with DC<\/jats:styled-content>s incubated with TSP<\/jats:styled-content>2HD<\/jats:styled-content> alone. Vaccination with TAT<\/jats:styled-content>\u2010TSP<\/jats:styled-content>2HD<\/jats:styled-content>\u2010pulsed DC<\/jats:styled-content>s in vivo<\/jats:italic> could improve disease outcome in S. japonicum\u2010<\/jats:italic>infected mice and was slightly superior to vaccination with DC<\/jats:styled-content>s treated with TSP<\/jats:styled-content>2HD<\/jats:styled-content>. In summary, these data showed that TAT<\/jats:styled-content> fusion proteins could help activate CD<\/jats:styled-content>8+<\/jats:sup> cells and Th1 cells and provide part protection against schistosome.<\/jats:p>","DOI":"10.1111\/pim.12338","type":"journal-article","created":{"date-parts":[[2016,5,18]],"date-time":"2016-05-18T12:55:31Z","timestamp":1463576131000},"page":"589-598","source":"Crossref","is-referenced-by-count":5,"title":["Protection and immunological study on two tetraspanin\u2010derived vaccine candidates against schistosomiasis japonicum"],"prefix":"10.1111","volume":"38","author":[{"given":"L.","family":"Chen","sequence":"first","affiliation":[{"name":"Department of Pathogen Biology Nanjing Medical University Nanjing Jiangsu China"},{"name":"Jiangsu Province Key Laboratory of Modern Pathogen Biology Nanjing Jiangsu China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Y.","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Pathogen Biology Nanjing Medical University Nanjing Jiangsu China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D.","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Pathogen Biology Nanjing Medical University Nanjing Jiangsu China"},{"name":"Jiangsu Province Key Laboratory of Modern Pathogen Biology Nanjing Jiangsu China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Hou","sequence":"additional","affiliation":[{"name":"Department of Pathogen Biology Nanjing Medical University Nanjing Jiangsu China"},{"name":"Jiangsu Province Key Laboratory of Modern Pathogen Biology Nanjing Jiangsu China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"B.","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Pathogen Biology Nanjing Medical University Nanjing Jiangsu China"},{"name":"Jiangsu Province Key Laboratory of Modern Pathogen Biology Nanjing Jiangsu China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"F.","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Pathogen Biology Nanjing Medical University Nanjing Jiangsu China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"W.","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Pathogen Biology Nanjing Medical University Nanjing Jiangsu China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"X.","family":"Luo","sequence":"additional","affiliation":[{"name":"Department of Pathogen Biology Nanjing Medical University Nanjing Jiangsu China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Ji","sequence":"additional","affiliation":[{"name":"Department of Pathogen Biology Nanjing Medical University Nanjing Jiangsu China"},{"name":"Jiangsu Province Key Laboratory of Modern Pathogen Biology Nanjing Jiangsu China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G.","family":"Wu","sequence":"additional","affiliation":[{"name":"Department of Pathogen Biology Nanjing Medical University Nanjing Jiangsu China"},{"name":"Jiangsu Province Key Laboratory of Modern Pathogen Biology Nanjing Jiangsu China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2016,6,13]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro2438"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(06)70521-7"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(06)69440-3"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cbpa.2007.05.031"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1186\/2049-9957-3-40"},{"key":"e_1_2_8_7_1","first-page":"591","article-title":"Endemic status of schistosomiasis in People's Republic of China in 2013","volume":"26","author":"Lei ZL","year":"2014","journal-title":"Zhongguo Xue Xi Chong Bing Fang Zhi Za Zhi"},{"key":"e_1_2_8_8_1","first-page":"1","article-title":"Surveillance system after transmission control of schistosomiasis in P.R. 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