{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,20]],"date-time":"2026-09-20T21:40:14Z","timestamp":1789940414655,"version":"4.0.1"},"reference-count":40,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2016,6,20]],"date-time":"2016-06-20T00:00:00Z","timestamp":1466380800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001700","name":"Ministry of Education, Culture, Sports, Science and Technology (MEXT) of Japan","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100001700","id-type":"DOI","asserted-by":"crossref"}]},{"name":"The Research Foundation for Pharmaceutical Sciences"},{"DOI":"10.13039\/100015060","name":"Nagasaki University","doi-asserted-by":"crossref","id":[{"id":"10.13039\/100015060","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Joint Research Promotion Project of Nagasaki University Graduate School of Biomedical Sciences"},{"name":"NEKKEN"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Parasite Immunology"],"published-print":{"date-parts":[[2016,10]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>Immune complexes (<jats:styled-content style=\"fixed-case\">IC<\/jats:styled-content>s) are the direct and real\u2010time products of humoral immune responses. The identification of constituent foreign or autoantigens within <jats:styled-content style=\"fixed-case\">IC<\/jats:styled-content>s might bring new insights into the pathology of infectious diseases. We applied immune complexome analysis of plasma to the study of Chagas disease caused by <jats:italic>Trypanosoma cruzi<\/jats:italic>. Twenty seropositive plasma samples including cardiac and\/or megacolon determinate patients (<jats:italic>n<\/jats:italic> = 11) and indeterminate (<jats:italic>n<\/jats:italic> = 9) were analysed along with 10 seronegative individuals to characterize the antigens bound to circulating <jats:styled-content style=\"fixed-case\">IC<\/jats:styled-content>s. We identified 39 <jats:italic>T. cruzi<\/jats:italic> antigens and 114 human autoantigens specific to patients with Chagas. Among those antigens, two <jats:italic>T. cruzi<\/jats:italic> antigens (surface protease <jats:styled-content style=\"fixed-case\">GP<\/jats:styled-content>63, glucose\u20106\u2010isomerase) and six human autoantigens (<jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>180 antigen, ceruloplasmin, fibrinogen beta chain, fibrinogen beta chain isoform 2 preprotein, isoform gamma\u2010A of fibrinogen \u03b3\u2010chain, serum paraoxonase) were detected in more than 50% of the patients tested. Human isoform short of complement factor H\u2010related protein 2 and trans\u2010sialidase of <jats:italic>T. cruzi<\/jats:italic> were more frequently found in the indeterminate (5\/9 for both) compared with in the determinate Chagas (0\/11, <jats:italic>P<\/jats:italic> = 0\u00b7046 for human, 1\/11, <jats:italic>P<\/jats:italic> = 0\u00b70498 for <jats:italic>T. cruzi<\/jats:italic>). The immune complexome could illustrate the difference of immune status between clinical forms of chronic Chagas disease.<\/jats:p>","DOI":"10.1111\/pim.12341","type":"journal-article","created":{"date-parts":[[2016,5,25]],"date-time":"2016-05-25T19:32:47Z","timestamp":1464204767000},"page":"609-617","source":"Crossref","is-referenced-by-count":30,"title":["Proteomic profile of circulating immune complexes in chronic Chagas disease"],"prefix":"10.1111","volume":"38","author":[{"given":"K.","family":"Ohyama","sequence":"first","affiliation":[{"name":"Course of Pharmaceutical Sciences Graduate School of Biomedical Sciences Nagasaki University Nagasaki Japan"},{"name":"Nagasaki University Research Centre for Genomic Instability and Carcinogenesis (NRGIC) Nagasaki Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"N. T.","family":"Huy","sequence":"additional","affiliation":[{"name":"Department of Clinical Product Development Institute of Tropical Medicine (NEKKEN), and Graduate School of Biomedical Sciences Nagasaki University Nagasaki Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H.","family":"Yoshimi","sequence":"additional","affiliation":[{"name":"Course of Pharmaceutical Sciences Graduate School of Biomedical Sciences Nagasaki University Nagasaki Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"N.","family":"Kishikawa","sequence":"additional","affiliation":[{"name":"Course of Pharmaceutical Sciences Graduate School of Biomedical Sciences Nagasaki University Nagasaki Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J. E.","family":"Nishizawa","sequence":"additional","affiliation":[{"name":"Clinica Siran\u00ed Santa Cruz Bolivia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Y.","family":"Roca","sequence":"additional","affiliation":[{"name":"Centro Nacional de Enfermedades Tropicales (CENETROP) Santa Cruz Bolivia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R. J.","family":"Revollo Guzm\u00e1n","sequence":"additional","affiliation":[{"name":"Centro Nacional de Enfermedades Tropicales (CENETROP) Santa Cruz Bolivia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"F. U. G.","family":"Velarde","sequence":"additional","affiliation":[{"name":"Hospital Universitario Japon\u00e9s Santa Cruz Bolivia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"N.","family":"Kuroda","sequence":"additional","affiliation":[{"name":"Course of Pharmaceutical Sciences Graduate School of Biomedical Sciences Nagasaki University Nagasaki Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K.","family":"Hirayama","sequence":"additional","affiliation":[{"name":"Department of Immunogenetics Institute of Tropical Medicine (NEKKEN), and Graduate School of Biomedical Sciences Nagasaki University Nagasaki 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