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Kinetoplastid membrane protein 11 (<jats:styled-content style=\"fixed-case\">KMP<\/jats:styled-content>11) is another protein antigen of <jats:italic>Leishmania<\/jats:italic> which is reported as a promising candidate for vaccination of VL. It is a highly conserved surface protein present in all members of kinetoplastid family and is expressed in both promastigotes and amastigotes. In this study, the coding sequence of <jats:styled-content style=\"fixed-case\">KMP<\/jats:styled-content>11 and <jats:styled-content style=\"fixed-case\">HASPB<\/jats:styled-content> was cloned into a <jats:styled-content style=\"fixed-case\">pCDH<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">cGFP<\/jats:styled-content> lentiviral vector as a fusion protein. The gene expression was confirmed using <jats:styled-content style=\"fixed-case\">RT<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content> and Western blot methods. After injection of the recombinant <jats:styled-content style=\"fixed-case\">KMP<\/jats:styled-content>11\u2010<jats:styled-content style=\"fixed-case\">HASPB<\/jats:styled-content>\u2010expressing lentiviruses to <jats:styled-content style=\"fixed-case\">BALB<\/jats:styled-content>\/c mice, using <jats:styled-content style=\"fixed-case\">ELISA<\/jats:styled-content> technique, a significant increase in <jats:styled-content style=\"fixed-case\">IFN<\/jats:styled-content>\u2010\u03b3 and <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u20104 as well as IgG1 and IgG2a was observed compared to the control group. Furthermore, the number of parasites in the liver and spleen of vaccinated mice decreased significantly compared with the control group.<\/jats:p>","DOI":"10.1111\/pim.12356","type":"journal-article","created":{"date-parts":[[2016,8,19]],"date-time":"2016-08-19T14:05:05Z","timestamp":1471615505000},"page":"670-677","source":"Crossref","is-referenced-by-count":13,"title":["Evaluation of a novel lentiviral vaccine expressing <scp>KMP<\/scp>11\u2010<scp>HASPB<\/scp> fusion protein against <i>Leishmania infantum<\/i> in <scp>BALB<\/scp>\/c mice"],"prefix":"10.1111","volume":"38","author":[{"given":"N.","family":"Mortazavidehkordi","sequence":"first","affiliation":[{"name":"Department of Parasitology and Mycology Isfahan University of Medical Sciences  Isfahan Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.","family":"Farjadfar","sequence":"additional","affiliation":[{"name":"Biotechnology Research Center Mede Bioeconomy Company  Tehran Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H.","family":"Khanahmad","sequence":"additional","affiliation":[{"name":"Department of Genetics Faculty of Medicine Isfahan University of Medical Sciences  Isfahan Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Z.","family":"Ghayour Najafabadi","sequence":"additional","affiliation":[{"name":"Department of Parasitology and Mycology Isfahan University of Medical Sciences  Isfahan Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"N.","family":"Hashemi","sequence":"additional","affiliation":[{"name":"Department of Parasitology and Mycology Isfahan University of Medical Sciences  Isfahan Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.","family":"Fallah","sequence":"additional","affiliation":[{"name":"Systems and Synthetic Biology Group Mede Bioeconomy Company  Tehran Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.","family":"Najafi","sequence":"additional","affiliation":[{"name":"Department of Immunology Pasteur Institute of Iran  Tehran Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"V.","family":"Kia","sequence":"additional","affiliation":[{"name":"Department of Medical Biotechnology Faculty of Medicine Zanjan University of Medical Sciences  Zanjan Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S. 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