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The results showed that <jats:styled-content style=\"fixed-case\">RCP<\/jats:styled-content>\/saponin\u2010vaccinated mice presented significantly higher levels of antileishmanial <jats:styled-content style=\"fixed-case\">IFN<\/jats:styled-content>\u2010\u03b3, <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u201012 and <jats:styled-content style=\"fixed-case\">GM<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">CSF<\/jats:styled-content> before and after challenge, which were associated with the reduction of <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u20104 and <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u201010 mediated responses. These animals showed significant reductions in the parasite burden in all evaluated organs, when both limiting dilution and quantitative real\u2010time <jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content> techniques were used. In addition, the protected animals presented higher levels of parasite\u2010specific nitrite, as well as the presence of anti\u2010<jats:italic>Leishmania<\/jats:italic> IgG2a isotype antibodies. In conclusion, the <jats:styled-content style=\"fixed-case\">RCP<\/jats:styled-content>\/saponin vaccine could be considered as a prophylactic alternative to prevent against <jats:styled-content style=\"fixed-case\">VL<\/jats:styled-content>.<\/jats:p>","DOI":"10.1111\/pim.12359","type":"journal-article","created":{"date-parts":[[2016,9,5]],"date-time":"2016-09-05T03:37:55Z","timestamp":1473046675000},"source":"Crossref","is-referenced-by-count":47,"title":["A recombinant chimeric protein composed of human and mice\u2010specific <scp>CD<\/scp>4<sup>+<\/sup> and <scp>CD<\/scp>8<sup>+<\/sup> T\u2010cell epitopes protects against visceral leishmaniasis"],"prefix":"10.1111","volume":"39","author":[{"given":"V. 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