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Skin lesions consist of ulcers with well\u2010defined raised edges, and granular floor. Th1 immune response is the protective profile in patients infected with Leishmania<\/jats:italic>. In this study, the photodynamic therapy with 5\u2010aminolevulinic acid, the parasitic load, and the modulation of the immune response was evaluated in mice infected with Leishmania braziliensis<\/jats:italic>. Balb\/c mice were infected with L. braziliensis<\/jats:italic> and subsequently treated with three sections of PDT<\/jats:styled-content>. The parasite load and mRNA<\/jats:styled-content> expression of cytokines (IFN<\/jats:styled-content>\u2010\u03b3, IL<\/jats:styled-content>\u20104, IL<\/jats:styled-content>\u201017, IL<\/jats:styled-content>\u201022, IL<\/jats:styled-content>\u201027, IL<\/jats:styled-content>\u201010) and transcription factors (GATA<\/jats:styled-content>\u20103, Foxp3 and T\u2010bet) were analysed by quantitative PCR<\/jats:styled-content>. The parasite load in the treated group was significantly lower than in the untreated group (P<\/jats:italic><.0001); in PDT<\/jats:styled-content> treated animals, we observed an increase in IFN<\/jats:styled-content>\u2010\u03b3 and T\u2010bet mRNA<\/jats:styled-content> (P<\/jats:italic>=.012 and P<\/jats:italic>=.0071). There was a significant reduction in mRNA<\/jats:styled-content> expression of IL<\/jats:styled-content>\u201022 associated with an increased expression of IL<\/jats:styled-content>\u201027 mRNA<\/jats:styled-content> in the animals treated with light only (P<\/jats:italic>=.0001). 5\u2010ALA<\/jats:styled-content> associated with photodynamic therapy promotes a reduction in parasite load and an increased expression of IFN<\/jats:styled-content>\u2010\u03b3 and T\u2010bet mRNA<\/jats:styled-content>.<\/jats:p>","DOI":"10.1111\/pim.12403","type":"journal-article","created":{"date-parts":[[2016,11,25]],"date-time":"2016-11-25T11:43:13Z","timestamp":1480074193000},"source":"Crossref","is-referenced-by-count":22,"title":["5\u2010ALA<\/scp>\u2010mediated photodynamic therapy reduces the parasite load in mice infected with Leishmania braziliensis<\/i>"],"prefix":"10.1111","volume":"39","author":[{"given":"D. M.","family":"Souza","sequence":"first","affiliation":[{"name":"Immunology Laboratory Department of Biological Sciences Federal University of Tri\u00e2ngulo Mineiro Uberaba Minas Gerais Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P. M.","family":"Alves","sequence":"additional","affiliation":[{"name":"Immunology Laboratory Department of Biological Sciences Federal University of Tri\u00e2ngulo Mineiro Uberaba Minas Gerais Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M. L. F.","family":"Silva","sequence":"additional","affiliation":[{"name":"Immunology Laboratory Department of Biological Sciences Federal University of Tri\u00e2ngulo Mineiro Uberaba Minas Gerais Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"T. P.","family":"Paulino","sequence":"additional","affiliation":[{"name":"Cefores \u2013 Centro de Educa\u00e7\u00e3o Profissional Federal University of Tri\u00e2ngulo Mineiro Uberaba Minas Gerais Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H. O.","family":"Coraspe","sequence":"additional","affiliation":[{"name":"Immunology Laboratory Department of Biological Sciences Federal University of Tri\u00e2ngulo Mineiro Uberaba Minas Gerais Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M. M. S.","family":"Mendon\u00e7a","sequence":"additional","affiliation":[{"name":"Immunology Laboratory Department of Biological Sciences Federal University of Tri\u00e2ngulo Mineiro Uberaba Minas Gerais Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"B. M.","family":"Ribeiro","sequence":"additional","affiliation":[{"name":"Immunology Laboratory Department of Biological Sciences Federal University of Tri\u00e2ngulo Mineiro Uberaba Minas Gerais Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M. V.","family":"da Silva","sequence":"additional","affiliation":[{"name":"Immunology Laboratory Department of Biological Sciences Federal University of Tri\u00e2ngulo Mineiro Uberaba Minas Gerais Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"V.","family":"Rodrigues J\u00fanior","sequence":"additional","affiliation":[{"name":"Immunology Laboratory Department of Biological Sciences Federal University of Tri\u00e2ngulo Mineiro Uberaba Minas Gerais Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D. B. R.","family":"Rodrigues","sequence":"additional","affiliation":[{"name":"Immunology Laboratory Department of Biological Sciences Federal University of Tri\u00e2ngulo Mineiro Uberaba Minas Gerais Brazil"},{"name":"Cefores \u2013 Centro de Educa\u00e7\u00e3o Profissional Federal University of Tri\u00e2ngulo Mineiro Uberaba Minas Gerais Brazil"},{"name":"Department of Immunology and Molecular Biology University of Uberaba Uberaba Minas Gerais Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2017,3,16]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00959-09"},{"key":"e_1_2_6_3_1","unstructured":"Minist\u00e9rio da Sa\u00fade.Secretaria de Vigil\u00e2ncia em Sa\u00fade. Manual de Vigil\u00e2ncia da Leishmaniose Tegumentar Americana [Internet]. 2010. p. 1\u2013180. Available from:http:\/\/bvsms.saude.gov.br\/bvs\/publicacoes\/manual_vigilancia_leishmaniose_tegumentar_americana.pdf. 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