{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,11]],"date-time":"2026-06-11T19:11:23Z","timestamp":1781205083180,"version":"3.54.1"},"reference-count":45,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2017,4,17]],"date-time":"2017-04-17T00:00:00Z","timestamp":1492387200000},"content-version":"vor","delay-in-days":16,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100004160","name":"Mazandaran University of Medical Sciences","doi-asserted-by":"publisher","award":["91\/327"],"award-info":[{"award-number":["91\/327"]}],"id":[{"id":"10.13039\/501100004160","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Parasite Immunology"],"published-print":{"date-parts":[[2017,4]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p><jats:italic>Toxoplasma gondii<\/jats:italic> can cause severe and even fatal disease in human beings and animals. Effective vaccines may contribute to control toxoplasmosis. <jats:styled-content style=\"fixed-case\">GRA<\/jats:styled-content>14, a novel secreted dense granule protein of <jats:italic>T.\u00a0gondii,<\/jats:italic> has been proposed as a vaccine candidate due to its intervacuolar transport and unique topology in the parasitophorous vacuole membrane. In this study, we constructed a <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> vaccine encoding <jats:styled-content style=\"fixed-case\">GRA<\/jats:styled-content>14 of <jats:italic>T.\u00a0gondii<\/jats:italic>. <jats:styled-content style=\"fixed-case\">BALB<\/jats:styled-content>\/c mice were immunized intramuscularly three times at 2\u00a0week intervals and challenged with <jats:italic>T.\u00a0gondii<\/jats:italic> <jats:styled-content style=\"fixed-case\">RH<\/jats:styled-content> strain 5\u00a0weeks later. The immune responses were evaluated using lymphocyte proliferation assay, cytokine and antibody measurements. In addition, the survival times and parasite load of mice challenged with the virulent <jats:italic>T.\u00a0gondii <\/jats:italic><jats:styled-content style=\"fixed-case\">RH<\/jats:styled-content> strain were evaluated. The results showed that the mice immunized with pc<jats:styled-content style=\"fixed-case\">GRA<\/jats:styled-content>14 induced both enhanced specific humoral and Th1 cellular immune responses, and also mice immunized with the pc<jats:styled-content style=\"fixed-case\">GRA<\/jats:styled-content>14 showed an increased survival time and decreased parasite load compared with control groups (<jats:italic>P<\/jats:italic>&lt;.05). The results indicated, for the first time, that the <jats:styled-content style=\"fixed-case\">GRA<\/jats:styled-content>14 is a potential <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> vaccine against toxoplasmosis.<\/jats:p>","DOI":"10.1111\/pim.12419","type":"journal-article","created":{"date-parts":[[2017,2,10]],"date-time":"2017-02-10T12:08:42Z","timestamp":1486728522000},"source":"Crossref","is-referenced-by-count":28,"title":["Evaluation of the immune response in <scp>BALB<\/scp>\/c mice induced by a novel <scp>DNA<\/scp> vaccine expressing <scp>GRA<\/scp>14 against <i>Toxoplasma gondii<\/i>"],"prefix":"10.1111","volume":"39","author":[{"given":"E.","family":"Ahmadpour","sequence":"first","affiliation":[{"name":"Immunology Research Center Tabriz University of Medical Sciences  Tabriz Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S.","family":"Sarvi","sequence":"additional","affiliation":[{"name":"Toxoplasmosis Research Center Mazandaran University of Medical Sciences  Sari Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M. 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