{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T18:33:35Z","timestamp":1790879615786,"version":"4.1.0"},"reference-count":38,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2017,5,29]],"date-time":"2017-05-29T00:00:00Z","timestamp":1496016000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","award":["Proep\/CNPq\/Fiocruz"],"award-info":[{"award-number":["Proep\/CNPq\/Fiocruz"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004586","name":"Funda\u00e7\u00e3o Carlos Chagas Filho de Amparo \u00e0 Pesquisa do Estado do Rio de Janeiro","doi-asserted-by":"publisher","award":["FAPERJ \u2013 APQ1 E\u201026\/111.727\/2012"],"award-info":[{"award-number":["FAPERJ \u2013 APQ1 E\u201026\/111.727\/2012"]}],"id":[{"id":"10.13039\/501100004586","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Parasite Immunology"],"published-print":{"date-parts":[[2017,7]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>The inflammasome is a multiprotein signalling platform involved in the pathogenesis of various inflammatory skin diseases. Herein, we investigated gene and protein expression of the inflammasome molecules <jats:styled-content style=\"fixed-case\">AIM<\/jats:styled-content>2 and <jats:styled-content style=\"fixed-case\">NLRP<\/jats:styled-content>3 in active lesions from patients with <jats:italic>L.\u00a0(V.)\u00a0braziliensis<\/jats:italic>\u2010associated tegumentary leishmaniasis (<jats:styled-content style=\"fixed-case\">TL<\/jats:styled-content>) and correlated these findings with the clinical presentations and responses to therapy. Real\u2010time <jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content> assays showed a significantly higher <jats:styled-content style=\"fixed-case\">AIM<\/jats:styled-content>2 gene expression in mucosal leishmaniasis (<jats:styled-content style=\"fixed-case\">ML<\/jats:styled-content>) compared with that in cutaneous leishmaniasis (<jats:styled-content style=\"fixed-case\">CL<\/jats:styled-content>). Additionally, <jats:styled-content style=\"fixed-case\">AIM<\/jats:styled-content>2 <jats:styled-content style=\"fixed-case\">mRNA<\/jats:styled-content> expression was significantly higher in lesions from poor responders than in lesions from good responders. In situ protein quantification analyses revealed greater <jats:styled-content style=\"fixed-case\">AIM<\/jats:styled-content>2 expression in <jats:styled-content style=\"fixed-case\">ML<\/jats:styled-content> lesions than in <jats:styled-content style=\"fixed-case\">CL<\/jats:styled-content> lesions. The percentage of <jats:styled-content style=\"fixed-case\">AIM<\/jats:styled-content>2\u2010producing cells was higher in poor responders than in good responders. Although not quite significant, <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u20101\u03b2+ cells were slightly more prominent in poor responders than in good responders. Similar results were observed when patients were evaluated according to clinical form. <jats:styled-content style=\"fixed-case\">GP<\/jats:styled-content>63 immunostaining was identified in all samples, but no significant variation between mucosal and cutaneous lesions was observed. <jats:styled-content style=\"fixed-case\">GP<\/jats:styled-content>63 could be associated with reduced <jats:styled-content style=\"fixed-case\">NLRP<\/jats:styled-content>3 inflammasome expression in <jats:styled-content style=\"fixed-case\">CL<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">ML<\/jats:styled-content> patients. Taken together, these data demonstrate that <jats:styled-content style=\"fixed-case\">AIM<\/jats:styled-content>2 is an important component of the inflammasome in <jats:styled-content style=\"fixed-case\">TL<\/jats:styled-content> patients and is directly associated with the severity of lesions.<\/jats:p>","DOI":"10.1111\/pim.12435","type":"journal-article","created":{"date-parts":[[2017,4,11]],"date-time":"2017-04-11T12:27:46Z","timestamp":1491913666000},"source":"Crossref","is-referenced-by-count":17,"title":["<scp>AIM<\/scp>2 inflammasome is associated with disease severity in tegumentary leishmaniasis caused by <i>Leishmania (V.) braziliensis<\/i>"],"prefix":"10.1111","volume":"39","author":[{"given":"R. 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