{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,19]],"date-time":"2026-08-19T17:24:11Z","timestamp":1787160251252,"version":"build-2736575974"},"reference-count":54,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2017,6,15]],"date-time":"2017-06-15T00:00:00Z","timestamp":1497484800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"\u201cSapienza-Research Grant\u201d","award":["C26H15SBJ4"],"award-info":[{"award-number":["C26H15SBJ4"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Parasite Immunology"],"published-print":{"date-parts":[[2017,7]]},"abstract":"SUMMARY<\/jats:title>\n \n I<\/jats:styled-content>\n g\n E<\/jats:styled-content>\n sensitization to\n \n A<\/jats:styled-content>\n nisakis pegreffii\n <\/jats:italic>\n in Italian subjects suffering from gastro\u2010allergic anisakiasis (\n GAA<\/jats:styled-content>\n ) (N=5), or showing chronic urticaria (\n CU<\/jats:styled-content>\n +) after fish consumption (N=100), was investigated. A control group (N=5) was also included.\n I<\/jats:styled-content>\n g\n E<\/jats:styled-content>\n response was analysed by immunoblotting (\n WB<\/jats:styled-content>\n ) assay, using both excretory\/secretory products (\n ESP<\/jats:styled-content>\n s) and crude extract (\n CE<\/jats:styled-content>\n ) of\n \n A<\/jats:styled-content>\n .\u00a0pegreffii\n <\/jats:italic>\n larvae. The results were compared with those achieved by the conventional immunological method for\n \n A<\/jats:styled-content>\n nisakis\n <\/jats:italic>\n allergy (ie, immuno\n CAP<\/jats:styled-content>\n ). Among the 110 subjects, 28 showed IgE positivity with both\n WB<\/jats:styled-content>\n and\n iCAP<\/jats:styled-content>\n methods; 13 proved IgE reactivity, in\n WB<\/jats:styled-content>\n assay, to\n ESP<\/jats:styled-content>\n antigens of\n \n A<\/jats:styled-content>\n .\u00a0pegreffii,\n <\/jats:italic>\n here provisionally indicated as\n \n Ani s 1<\/jats:italic>\n <\/jats:styled-content>\n \u2010<\/jats:italic>\n like,\n \n Ani s 7<\/jats:italic>\n <\/jats:styled-content>\n \u2010<\/jats:italic>\n like,\n \n Ani s 13<\/jats:italic>\n <\/jats:styled-content>\n \u2010<\/jats:italic>\n like; only 15 sera have shown IgE\u2010\n WB<\/jats:styled-content>\n reaction to\n \n Ani s 7<\/jats:italic>\n <\/jats:styled-content>\n \u2010<\/jats:italic>\n like and\n \n Ani s 13<\/jats:italic>\n <\/jats:styled-content>\n \u2010<\/jats:italic>\n like.\n iCAP<\/jats:styled-content>\n and\n WB<\/jats:styled-content>\n exhibited a high concordance value (\u03ba=1.00) when\n iCAP<\/jats:styled-content>\n value was <0.35 (negative result) and >50.0 (positive result). In the sera samples recorded as positive to\n \n A<\/jats:styled-content>\n nisakis\n <\/jats:italic>\n allergy,\n \n Ani s 1<\/jats:italic>\n <\/jats:styled-content>\n \u2010<\/jats:italic>\n like was responsible for 46.4% of the sensitivity, while\n \n Ani s 7<\/jats:italic>\n <\/jats:styled-content>\n \u2010<\/jats:italic>\n like and\n \n Ani s 13<\/jats:italic>\n <\/jats:styled-content>\n \u2010<\/jats:italic>\n like for 100%. They could be considered as major antigens in the diagnosis of allergic anisakiasis caused by\n \n A<\/jats:styled-content>\n .\u00a0pegreffii\n <\/jats:italic>\n .\n <\/jats:p>","DOI":"10.1111\/pim.12440","type":"journal-article","created":{"date-parts":[[2017,5,5]],"date-time":"2017-05-05T09:06:59Z","timestamp":1493975219000},"source":"Crossref","is-referenced-by-count":38,"title":["I<\/scp>\n g\n E<\/scp>\n sensitization to\n \n A<\/scp>\n nisakis pegreffii\n <\/i>\n in\n I<\/scp>\n taly: Comparison of two methods for the diagnosis of allergic anisakiasis"],"prefix":"10.1111","volume":"39","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3881-095X","authenticated-orcid":false,"given":"S.","family":"Mattiucci","sequence":"first","affiliation":[{"name":"Section of Parasitology Department of Public Health and Infectious Diseases \u201cSapienza \u2010 University of Rome\u201d and \u201cUmberto I\u201d University Hospital Rome Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.","family":"Colantoni","sequence":"additional","affiliation":[{"name":"Section of Parasitology Department of Public Health and Infectious Diseases \u201cSapienza \u2010 University of Rome\u201d and \u201cUmberto I\u201d University Hospital Rome Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"B.","family":"Crisafi","sequence":"additional","affiliation":[{"name":"Section of Parasitology Department of Public Health and Infectious Diseases \u201cSapienza \u2010 University of Rome\u201d and \u201cUmberto I\u201d University Hospital Rome Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"F.","family":"Mori\u2010Ubaldini","sequence":"additional","affiliation":[{"name":"Department of Translational Research N.T.M.S., Pisa University Pisa Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L.","family":"Caponi","sequence":"additional","affiliation":[{"name":"Department of Translational Research N.T.M.S., Pisa University Pisa Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P.","family":"Fazii","sequence":"additional","affiliation":[{"name":"\u201c<\/i>Santo Spirito\u201d Hospital Pescara Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G.","family":"Nascetti","sequence":"additional","affiliation":[{"name":"Department of Ecological and Biological Sciences Tuscia University Viterbo Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"F.","family":"Bruschi","sequence":"additional","affiliation":[{"name":"Department of Translational Research N.T.M.S., Pisa University Pisa Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2017,6,15]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.2903\/j.efsa.2010.1543"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4612-2732-8_3"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00012-07"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1097\/01.all.0000168795.12701.fd"},{"key":"e_1_2_8_6_1","doi-asserted-by":"crossref","first-page":"341","DOI":"10.1016\/S0210-5705(03)70370-7","article-title":"Gastrointestinal anisakiasis. 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