{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,27]],"date-time":"2025-10-27T20:44:00Z","timestamp":1761597840082,"version":"3.41.2"},"reference-count":48,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2017,8,18]],"date-time":"2017-08-18T00:00:00Z","timestamp":1503014400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100005851","name":"Shahid Beheshti University of Medical Sciences","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100005851","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003968","name":"Iran National Science Foundation","doi-asserted-by":"publisher","award":["94013422","940007"],"award-info":[{"award-number":["94013422","940007"]}],"id":[{"id":"10.13039\/501100003968","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Parasite Immunology"],"published-print":{"date-parts":[[2017,9]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>Cutaneous leishmaniasis (<jats:styled-content style=\"fixed-case\">CL<\/jats:styled-content>) is one of the most important vector\u2010borne parasitic diseases, highly endemic in Iran, and its prevalence is increasing all over the country. Arginase (<jats:styled-content style=\"fixed-case\">ARG<\/jats:styled-content>) activity in isolated<jats:italic>Leishmania<\/jats:italic>parasites from<jats:styled-content style=\"fixed-case\">CL<\/jats:styled-content>patients is yet to be explored. This study aimed to compare the<jats:styled-content style=\"fixed-case\">ARG<\/jats:styled-content>activity of isolated<jats:italic>Leishmania<\/jats:italic>promastigotes from<jats:styled-content style=\"fixed-case\">CL<\/jats:styled-content>patients with a standard strain of<jats:italic>Leishmania major<\/jats:italic>and its influences on the disease pathogenesis. We recruited 16 confirmed<jats:styled-content style=\"fixed-case\">CL<\/jats:styled-content>patients from Qom Province, in central Iran; after detection of<jats:italic>Leishmania<\/jats:italic>species using<jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">RFLP<\/jats:styled-content>, we assessed the levels of<jats:styled-content style=\"fixed-case\">ARG<\/jats:styled-content>in the isolated promastigotes and determined the parasites\u2019 growth rate. Only<jats:italic>L.\u00a0major<\/jats:italic>was identified from<jats:styled-content style=\"fixed-case\">CL<\/jats:styled-content>patients. The level of<jats:styled-content style=\"fixed-case\">ARG<\/jats:styled-content>activity in the isolated<jats:italic>Leishmania<\/jats:italic>promastigotes from<jats:styled-content style=\"fixed-case\">CL<\/jats:styled-content>patients was significantly higher than that obtained from the standard strain of<jats:italic>L.\u00a0major<\/jats:italic>. No significant correlations between<jats:styled-content style=\"fixed-case\">ARG<\/jats:styled-content>activity and lesion size, number or duration were observed; in contrast, a significant negative correlation was seen between<jats:styled-content style=\"fixed-case\">ARG<\/jats:styled-content>level and<jats:italic>Leishmania\u2019<\/jats:italic>growth rate. The obtained results suggest that increased<jats:styled-content style=\"fixed-case\">ARG<\/jats:styled-content>expression and activity in the isolated<jats:italic>Leishmania<\/jats:italic>promastigotes might contribute to the higher parasite infectivity and play a major role in the pathogenicity of the<jats:styled-content style=\"fixed-case\">CL<\/jats:styled-content>.<\/jats:p>","DOI":"10.1111\/pim.12454","type":"journal-article","created":{"date-parts":[[2017,7,21]],"date-time":"2017-07-21T14:42:38Z","timestamp":1500648158000},"source":"Crossref","is-referenced-by-count":11,"title":["Arginase activity of<i>Leishmania<\/i>isolated from patients with cutaneous leishmaniasis"],"prefix":"10.1111","volume":"39","author":[{"given":"A.","family":"Badirzadeh","sequence":"first","affiliation":[{"name":"Department of Medical Parasitology and Mycology School of Medicine Shahid Beheshti University of Medical Sciences Tehran Iran"},{"name":"Department of Immunotherapy and <i>Leishmania<\/i> Vaccine Research Pasteur Institute of Iran Tehran Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"T.","family":"Taheri","sequence":"additional","affiliation":[{"name":"Department of Immunotherapy and <i>Leishmania<\/i> Vaccine Research Pasteur Institute of Iran Tehran Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"F.","family":"Abedi\u2010Astaneh","sequence":"additional","affiliation":[{"name":"Department of Communicable Disease Deputy of Health Qom University of Medical Sciences Qom Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Y.","family":"Taslimi","sequence":"additional","affiliation":[{"name":"Department of Immunotherapy and <i>Leishmania<\/i> Vaccine Research Pasteur Institute of Iran Tehran Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Z.","family":"Abdossamadi","sequence":"additional","affiliation":[{"name":"Department of Immunotherapy and <i>Leishmania<\/i> Vaccine Research Pasteur Institute of Iran Tehran Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H.","family":"Montakhab\u2010Yeganeh","sequence":"additional","affiliation":[{"name":"Department of Immunotherapy and <i>Leishmania<\/i> Vaccine Research Pasteur Institute of Iran Tehran Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Aghashahi","sequence":"additional","affiliation":[{"name":"Department of Communicable Disease Deputy of Health Qom University of Medical Sciences Qom Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Niyyati","sequence":"additional","affiliation":[{"name":"Department of Medical Parasitology and Mycology School of Medicine Shahid Beheshti University of Medical Sciences Tehran Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7221-1320","authenticated-orcid":false,"given":"S.","family":"Rafati","sequence":"additional","affiliation":[{"name":"Department of Immunotherapy and <i>Leishmania<\/i> Vaccine Research Pasteur Institute of Iran Tehran Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2017,8,18]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(16)00003-7"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1080\/20477724.2017.1308902"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/pim.12423"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cellimm.2016.10.001"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/pim.12304"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/pim.12420"},{"key":"e_1_2_7_8_1","unstructured":"Meeting WECotCotL. 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