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The <jats:italic>Schistosoma mansoni <\/jats:italic><jats:styled-content style=\"fixed-case\">NTPD<\/jats:styled-content>ases<jats:italic>,<\/jats:italic> Sm<jats:styled-content style=\"fixed-case\">ATPD<\/jats:styled-content>ases 1 and 2, are antigenic proteins and display a significant homology with the isoforms found in mammalian cells. In this work, we investigated whether anti\u2010Sm<jats:styled-content style=\"fixed-case\">ATPD<\/jats:styled-content>ase antibodies from <jats:italic>S.\u00a0mansoni\u2010<\/jats:italic>infected mice sera show cross\u2010reactivity with the <jats:styled-content style=\"fixed-case\">NTPD<\/jats:styled-content>ase 1 isoform from macrophages and how this event affects the cell proliferation. By Western blot, anti\u2010Sm<jats:styled-content style=\"fixed-case\">ATPD<\/jats:styled-content>ase antibodies present in serum from infected mice recognized 2 bands with approximately 53 and 58 <jats:styled-content style=\"fixed-case\">kD<\/jats:styled-content>a, corresponding to <jats:styled-content style=\"fixed-case\">NTPD<\/jats:styled-content>ase 1. Additionally, the enzyme was identified in macrophages by immunofluorescence and the anti\u2010Sm<jats:styled-content style=\"fixed-case\">ATPD<\/jats:styled-content>ase antibodies were able to reduce activity enzyme (22%). Macrophages incubated with commercial polyclonal antibodies reactive with <jats:styled-content style=\"fixed-case\">NTPD<\/jats:styled-content>ase 1 (anti\u2010<jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>39) showed a reduction of 40% of the enzyme activity. In proliferation assays, macrophage proliferation was inhibited 11% and 90% by pooled sera from infected animals and anti\u2010<jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>39, respectively. The results suggest that inhibition of <jats:styled-content style=\"fixed-case\">NTPD<\/jats:styled-content>ase 1 in macrophages by antibodies produced against the isoforms of the <jats:italic>S.\u00a0mansoni <\/jats:italic><jats:styled-content style=\"fixed-case\">ATPD<\/jats:styled-content>ases could be a mechanism of regulation in the immune response during experimental schistosomiasis.<\/jats:p>","DOI":"10.1111\/pim.12487","type":"journal-article","created":{"date-parts":[[2017,8,31]],"date-time":"2017-08-31T11:52:13Z","timestamp":1504180333000},"source":"Crossref","is-referenced-by-count":3,"title":["Antischistosome antibodies change <scp>NTPD<\/scp>ase 1 activity from macrophages"],"prefix":"10.1111","volume":"39","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8186-3486","authenticated-orcid":false,"given":"D. 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M. de A.","family":"Castro","sequence":"additional","affiliation":[{"name":"Department of Immunology, Parasitology and Microbiology Institute of Biological Sciences Federal University of Juiz de Fora  Juiz de Fora MG Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G. C.","family":"Macedo","sequence":"additional","affiliation":[{"name":"Department of Immunology, Parasitology and Microbiology Institute of Biological Sciences Federal University of Juiz de Fora  Juiz de Fora MG Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"E. 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