{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T07:45:44Z","timestamp":1789717544460,"version":"4.0.1"},"reference-count":24,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2017,10,9]],"date-time":"2017-10-09T00:00:00Z","timestamp":1507507200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100002923","name":"Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas","doi-asserted-by":"publisher","award":["CONICET PIP 11220150100210Co 2015\u20102017"],"award-info":[{"award-number":["CONICET PIP 11220150100210Co 2015\u20102017"]}],"id":[{"id":"10.13039\/501100002923","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Parasite Immunology"],"published-print":{"date-parts":[[2017,11]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>Our proposal was to develop a vaccine based on total <jats:italic>Leishmania<\/jats:italic> antigens (<jats:styled-content style=\"fixed-case\">TLA<\/jats:styled-content>) adjuvanted with polyinosinic\u2010polycytidylic acid [Poly(I:C)] able to induce a Th1 response which can provide protection against <jats:italic>Leishmania<\/jats:italic> infection. Mice were vaccinated with two doses of <jats:styled-content style=\"fixed-case\">TLA<\/jats:styled-content>\u2010Poly(I:C) administered by subcutaneous route at 3\u2010week interval. Humoral and cellular immune responses induced by the immunization were measured. The protective efficacy of the vaccine was evaluated by challenging mice with infective promastigotes of <jats:italic>Leishmania (Leishmania) amazonensis<\/jats:italic> into the footpad. Mice vaccinated with <jats:styled-content style=\"fixed-case\">TLA<\/jats:styled-content>\u2010Poly(I:C) showed a high anti\u2010<jats:italic>Leishmania<\/jats:italic> IgG titre, as well as increased IgG1 and IgG2a subclass titres compared with mice vaccinated with the <jats:styled-content style=\"fixed-case\">TLA<\/jats:styled-content> alone. The high IgG2a indicated a Th1 bias response induced by the <jats:styled-content style=\"fixed-case\">TLA<\/jats:styled-content>\u2010Poly(I:C) immunization. Accordingly, the cellular immune response elicited by the formulation was characterized by an increased production of <jats:styled-content style=\"fixed-case\">IFN<\/jats:styled-content>\u2010\u03b3 and no significant production of <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u20104. The <jats:styled-content style=\"fixed-case\">TLA<\/jats:styled-content>\u2010Poly(I:C) immunization elicited good protection, which was associated with decreased footpad swelling, a lower parasite load and a reduced histopathological alteration in the footpad. Our findings demonstrate a promising vaccine against cutaneous leishmaniasis that is relatively economic and easy to develop and which should be taken into account for preventing leishmaniasis in developing countries.<\/jats:p>","DOI":"10.1111\/pim.12491","type":"journal-article","created":{"date-parts":[[2017,9,13]],"date-time":"2017-09-13T11:22:57Z","timestamp":1505301777000},"source":"Crossref","is-referenced-by-count":16,"title":["Total <i>Leishmania<\/i> antigens with Poly(I:C) induce Th1 protective response"],"prefix":"10.1111","volume":"39","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2252-959X","authenticated-orcid":false,"given":"M. V.","family":"Sanchez","sequence":"first","affiliation":[{"name":"Instituto de Medicina y Biolog\u00eda Experimental de Cuyo (IMBECU) Centro Cient\u00edfico y Tecnol\u00f3gico de Mendoza (CCT\u2010Mendoza) Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas (CONICET)  Mendoza Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R. J.","family":"Eli\u00e7abe","sequence":"additional","affiliation":[{"name":"Instituto Multidisciplinario de Investigaciones Biol\u00f3gicas San Luis (IMIBIO\u2010SL) Centro Cient\u00edfico y Tecnol\u00f3gico de San Luis (CCT\u2010San Luis) Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas (CONICET)  Ciudad de San Luis San Luis Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M. S.","family":"Di Genaro","sequence":"additional","affiliation":[{"name":"Instituto Multidisciplinario de Investigaciones Biol\u00f3gicas San Luis (IMIBIO\u2010SL) Centro Cient\u00edfico y Tecnol\u00f3gico de San Luis (CCT\u2010San Luis) Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas (CONICET)  Ciudad de San Luis San Luis Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M. J.","family":"German\u00f3","sequence":"additional","affiliation":[{"name":"Instituto de Medicina y Biolog\u00eda Experimental de Cuyo (IMBECU) Centro Cient\u00edfico y Tecnol\u00f3gico de Mendoza (CCT\u2010Mendoza) Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas (CONICET)  Mendoza Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S.","family":"Gea","sequence":"additional","affiliation":[{"name":"Centro de Investigaciones en Bioqu\u00edmica Cl\u00ednica e Inmunolog\u00eda (CIBICI) Centro Cient\u00edfico y Tecnol\u00f3gico de C\u00f3rdoba (CCT\u2010C\u00f3rdoba) Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas (CONICET)  Buenos Aires C\u00f3rdoba Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M. F.","family":"Garc\u00eda Bustos","sequence":"additional","affiliation":[{"name":"Instituto de Patolog\u00eda Experimental (IPE) Centro Cient\u00edfico y Tecnol\u00f3gico de Salta (CCT\u2010Salta) Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas (CONICET)  Salta Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M. C.","family":"Salom\u00f3n","sequence":"additional","affiliation":[{"name":"Area de Parasitolog\u00eda Facultad de Ciencias M\u00e9dicas Universidad Nacional de Cuyo (UNCuyo) UNCUYO Centro Universitario (M5502JMA)  Mendoza Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"E. A.","family":"Scodeller","sequence":"additional","affiliation":[{"name":"Instituto de Medicina y Biolog\u00eda Experimental de Cuyo (IMBECU) Centro Cient\u00edfico y Tecnol\u00f3gico de Mendoza (CCT\u2010Mendoza) Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas (CONICET)  Mendoza Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D. E.","family":"Cargnelutti","sequence":"additional","affiliation":[{"name":"Instituto de Medicina y Biolog\u00eda Experimental de Cuyo (IMBECU) Centro Cient\u00edfico y Tecnol\u00f3gico de Mendoza (CCT\u2010Mendoza) Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas (CONICET)  Mendoza Argentina"},{"name":"Area de Parasitolog\u00eda Facultad de Ciencias M\u00e9dicas Universidad Nacional de Cuyo (UNCuyo) UNCUYO Centro Universitario (M5502JMA)  Mendoza Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2017,10,9]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0035671"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/cti.2014.4"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1590\/S0074-02762004000100010"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1093\/trstmh\/trt006"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2005.02.015"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0002148"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2012.11.068"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1155\/2016\/1459394"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/35099560"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2010.08.098"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1158\/2326-6066.CIR-13-0089"},{"key":"e_1_2_7_13_1","unstructured":"BhardwajN.Safety Study of Adjuvant Vaccine to Treat Melanoma Patients [Internet]. 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