{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,23]],"date-time":"2025-12-23T15:39:13Z","timestamp":1766504353425,"version":"3.41.2"},"reference-count":45,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2017,11,24]],"date-time":"2017-11-24T00:00:00Z","timestamp":1511481600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003196","name":"Ministero della Salute","doi-asserted-by":"publisher","award":["Linea 1, Ricerca Corrente"],"award-info":[{"award-number":["Linea 1, Ricerca Corrente"]}],"id":[{"id":"10.13039\/501100003196","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100011272","name":"FP7 Health","doi-asserted-by":"publisher","award":["HEALTH\u2010F3\u20102009\u2010241642"],"award-info":[{"award-number":["HEALTH\u2010F3\u20102009\u2010241642"]}],"id":[{"id":"10.13039\/100011272","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Parasite Immunology"],"published-print":{"date-parts":[[2017,12]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>Cystic echinococcosis (<jats:styled-content style=\"fixed-case\">CE<\/jats:styled-content>) immunodiagnosis is still imperfect. We recently set\u2010up a whole\u2010blood test based on the interleukin (<jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>)\u20104 response to the native Antigen B (AgB) of <jats:italic>Echinococcus granulosus<\/jats:italic>. However, AgB is encoded by a multigene family coding for five putative subunits. Therefore, the aims of this study were to analyse the <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u20104 response to peptides spanning the immunodominant regions of the five AgB subunits and to evaluate the accuracy of this assay for <jats:styled-content style=\"fixed-case\">CE<\/jats:styled-content> diagnosis. Peptides corresponding to each subunit were combined into five pools. A pool containing all peptides was also used (total pool). <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u20104 evaluated by enzyme\u2010linked immunosorbent assay was significantly higher in patients with <jats:styled-content style=\"fixed-case\">CE<\/jats:styled-content> compared to those without (<jats:styled-content style=\"fixed-case\">NO<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">CE<\/jats:styled-content> subjects) when whole\u2010blood was stimulated with AgB1 and with the total pool. Moreover, <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u20104 levels in response to the total pool were significantly increased in patients with active cysts. Receiver Operator Curve analysis identified a cut\u2010off point of 0.59\u00a0pg\/<jats:styled-content style=\"fixed-case\">mL<\/jats:styled-content> predicting active cysts diagnosis with 71% sensitivity and 82% specificity in serology\u2010positive <jats:styled-content style=\"fixed-case\">CE<\/jats:styled-content> patients. These data, if confirmed in a larger cohort, offer the opportunity to develop new diagnostic tools for <jats:styled-content style=\"fixed-case\">CE<\/jats:styled-content> based on a standardized source of AgB as the peptides.<\/jats:p>","DOI":"10.1111\/pim.12499","type":"journal-article","created":{"date-parts":[[2017,10,27]],"date-time":"2017-10-27T16:46:34Z","timestamp":1509122794000},"source":"Crossref","is-referenced-by-count":15,"title":["A T\u2010cell diagnostic test for cystic echinococcosis based on Antigen B peptides"],"prefix":"10.1111","volume":"39","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2593-3478","authenticated-orcid":false,"given":"L.","family":"Petrone","sequence":"first","affiliation":[{"name":"Translational Research Unit Department of Epidemiology and Preclinical Research \u201cL. 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