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In all immunized groups, the percentage of CD<\/jats:styled-content>4 was higher than CD<\/jats:styled-content>8 cells. CD<\/jats:styled-content>45 was increased in B cells from mice immunized. We observed IgA antibody\u2010forming cell (IgA\u2010AFC<\/jats:styled-content>) response, mainly in NALT<\/jats:styled-content> and NP<\/jats:styled-content>. Macrophages from NP<\/jats:styled-content> and CN<\/jats:styled-content> expressed the highest levels of CD<\/jats:styled-content>80 and CD<\/jats:styled-content>86 in N.\u00a0fowleri<\/jats:italic> lysates with either CT<\/jats:styled-content> or CT<\/jats:styled-content> alone immunized mice, whereas dendritic cells expressed high levels of CD<\/jats:styled-content>80 and CD<\/jats:styled-content>86 in all compartment from immunized mice. These were lower than those expressed by macrophages. Only in SP<\/jats:styled-content> from CT<\/jats:styled-content>\u2010immunized mice, these costimulatory molecules were increased. These results suggest that N.\u00a0fowleri<\/jats:italic> and CT<\/jats:styled-content> antigens are taking by APC<\/jats:styled-content>s, and therefore, protective immunity depends on interactions between APC<\/jats:styled-content>s and T cells from NP<\/jats:styled-content> and CN<\/jats:styled-content>. Consequently, CD<\/jats:styled-content>4 cells stimulate the differentiation from B lymphocytes to AFC<\/jats:styled-content> IgA\u2010positive; antibody that we previously found interacting with trophozoites in the nasal lumen avoiding the N.\u00a0fowleri<\/jats:italic> attachment to nasal epithelium.<\/jats:p>","DOI":"10.1111\/pim.12508","type":"journal-article","created":{"date-parts":[[2017,12,16]],"date-time":"2017-12-16T21:29:20Z","timestamp":1513459760000},"source":"Crossref","is-referenced-by-count":10,"title":["Naegleria fowleri<\/i> immunization modifies lymphocytes and APC<\/scp> of nasal mucosa"],"prefix":"10.1111","volume":"40","author":[{"given":"M. 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