{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T23:59:36Z","timestamp":1791331176621,"version":"4.3.1"},"reference-count":22,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2018,4,14]],"date-time":"2018-04-14T00:00:00Z","timestamp":1523664000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100003554","name":"Lundbeckfonden","doi-asserted-by":"publisher","award":["14\u20103670A"],"award-info":[{"award-number":["14\u20103670A"]}],"id":[{"id":"10.13039\/501100003554","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Parasite Immunology"],"published-print":{"date-parts":[[2018,5]]},"abstract":"Summary<\/jats:title>\n \n Giardia duodenalis<\/jats:italic>\n is a common intestinal protozoan parasite known to modulate host immune responses, including dendritic cell (\n DC<\/jats:styled-content>\n ) function. Coinfections of intestinal pathogens are common, and thus,\n DC<\/jats:styled-content>\n s may be concurrently exposed to antigens from multiple parasites. Here, we investigated the effects of\n G.\u00a0duodenalis<\/jats:italic>\n products on human monocyte\u2010derived\n DC<\/jats:styled-content>\n function independently and in combination with helminth antigens (\n Ascaris suum<\/jats:italic>\n and\n Trichuris suis<\/jats:italic>\n ). All antigens individually induced an anti\u2010inflammatory phenotype in\n DC<\/jats:styled-content>\n s, reducing lipopolysaccharide (\n LPS<\/jats:styled-content>\n )\u2010induced interleukin (\n IL<\/jats:styled-content>\n )\u20106,\n IL<\/jats:styled-content>\n \u201012p70 and tumour necrosis factor (\n TNF<\/jats:styled-content>\n )\u2010\u03b1 secretion.\n G.\u00a0duodenalis<\/jats:italic>\n and\n T.\u00a0suis<\/jats:italic>\n products also consistently upregulated\n IL<\/jats:styled-content>\n \u201010 production. Despite a similar modulation of cytokine secretion, additive effects between\n Giardia<\/jats:italic>\n and helminth products were not observed, indicating a dominant effect of a single parasite stimulus and limited interactive effects on\n DC<\/jats:styled-content>\n function.\n G.\u00a0duodenalis<\/jats:italic>\n trophozoites induced rapid apoptosis in\n DC<\/jats:styled-content>\n s, which was not observed with the helminth antigens suggesting that the modulatory effects of\n G.\u00a0duodenalis<\/jats:italic>\n may override that of\n A.\u00a0suum<\/jats:italic>\n and\n T.\u00a0suis<\/jats:italic>\n . Thus,\n G.\u00a0duodenalis<\/jats:italic>\n modulates\n DC<\/jats:styled-content>\n activity by modulating cytokine secretion and\/or inducing apoptosis, which may be a parasite\u2010driven mechanism to dampen host immunity and establish chronic infections. The differential mechanisms of\n DC<\/jats:styled-content>\n modulation by intestinal parasites warrant further attention.\n <\/jats:p>","DOI":"10.1111\/pim.12525","type":"journal-article","created":{"date-parts":[[2018,3,25]],"date-time":"2018-03-25T09:42:05Z","timestamp":1521970925000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["Modulation of human dendritic cell activity by\n Giardia<\/i>\n and helminth antigens"],"prefix":"10.1111","volume":"40","author":[{"given":"A.","family":"Summan","sequence":"first","affiliation":[{"name":"Department of Veterinary and Animal Sciences University of Copenhagen Frederiksberg Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P.","family":"Nejsum","sequence":"additional","affiliation":[{"name":"Department of Clinical Medicine Aarhus University Aarhus Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8231-282X","authenticated-orcid":false,"given":"A. R.","family":"Williams","sequence":"additional","affiliation":[{"name":"Department of Veterinary and Animal Sciences University of Copenhagen Frederiksberg Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2018,4,14]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.immunol.18.1.767"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-5822.2006.00796.x"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1186\/1756-3305-6-317"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00718-08"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0003680"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.imbio.2007.03.009"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.69.3.1574-1580.2001"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2009.05.075"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijpara.2012.10.021"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijpara.2010.09.007"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/jix585"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.16-0580"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1111\/imm.12687"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetimm.2017.06.004"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1038\/gene.2015.18"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1111\/pim.12214"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.1996.471410.x"},{"key":"e_1_2_7_19_1","first-page":"e779","article-title":"Macrophage cell death upon intracellular bacterial infection","volume":"2","author":"Lai X\u2010H","year":"2015","journal-title":"Macrophage"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.exppara.2010.06.014"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.70.7.3673-3680.2002"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20121972"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1017\/S0031182017000841"}],"container-title":["Parasite Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fpim.12525","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/pim.12525","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,24]],"date-time":"2023-09-24T12:05:50Z","timestamp":1695557150000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/pim.12525"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,4,14]]},"references-count":22,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2018,5]]}},"alternative-id":["10.1111\/pim.12525"],"URL":"https:\/\/doi.org\/10.1111\/pim.12525","archive":["Portico"],"relation":{},"ISSN":["0141-9838","1365-3024"],"issn-type":[{"value":"0141-9838","type":"print"},{"value":"1365-3024","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,4,14]]},"assertion":[{"value":"2017-12-18","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-03-12","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-04-14","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e12525"}}