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These modifications frequently elicited a polarization towards Th2 cells and regulatory T cells thus contributing to immunological tolerance against these pathogens. In this study, the interaction between <jats:styled-content style=\"fixed-case\">DC<\/jats:styled-content>s and larvae of the zoonotic anisakid nematode <jats:italic>Anisakis pegreffii<\/jats:italic> was investigated. <jats:italic>A.\u00a0pegreffii<\/jats:italic> larvae were collected from fish hosts, and monocyte\u2010derived <jats:styled-content style=\"fixed-case\">DC<\/jats:styled-content>s were cocultured in the presence of the live larvae (L) or its crude extracts (<jats:styled-content style=\"fixed-case\">CE<\/jats:styled-content>). In both experimental conditions, <jats:italic>A.\u00a0pegreffii<\/jats:italic> impacted <jats:styled-content style=\"fixed-case\">DC<\/jats:styled-content> viability, hampered <jats:styled-content style=\"fixed-case\">DC<\/jats:styled-content> maturation by reducing the expression of molecules involved in antigen presentation and migration (ie <jats:styled-content style=\"fixed-case\">HLA<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">DR<\/jats:styled-content>,<jats:styled-content style=\"fixed-case\"> CD<\/jats:styled-content>86, <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>83 and <jats:styled-content style=\"fixed-case\">CCR<\/jats:styled-content>7), increased the phagosomal radical oxygen species (<jats:styled-content style=\"fixed-case\">ROS<\/jats:styled-content>) levels and modulated the phosphorylation of <jats:styled-content style=\"fixed-case\">ERK<\/jats:styled-content>1,2 pathway. These biological changes were accompanied by the impairment of <jats:styled-content style=\"fixed-case\">DC<\/jats:styled-content>s to activate a T\u2010cell\u2010mediated <jats:styled-content style=\"fixed-case\">IFN<\/jats:styled-content>\u03b3. Interestingly, live larvae appeared to differently modulate <jats:styled-content style=\"fixed-case\">DC<\/jats:styled-content> secretion of cytokines and chemokines as compared to <jats:styled-content style=\"fixed-case\">CE<\/jats:styled-content>. These results demonstrate, for the first time, the immunomodulatory role of <jats:italic>A.\u00a0pegreffii<\/jats:italic> on <jats:styled-content style=\"fixed-case\">DC<\/jats:styled-content>s biology and functions. In addition, they suggest a dynamic contribution of <jats:styled-content style=\"fixed-case\">DC<\/jats:styled-content>s to the induction and maintenance of the inflammatory response against <jats:italic>A.\u00a0pegreffii<\/jats:italic>.<\/jats:p>","DOI":"10.1111\/pim.12527","type":"journal-article","created":{"date-parts":[[2018,3,23]],"date-time":"2018-03-23T11:18:56Z","timestamp":1521803936000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":28,"title":["<i>Anisakis pegreffii<\/i> impacts differentiation and function of human dendritic cells"],"prefix":"10.1111","volume":"40","author":[{"given":"C.","family":"Napoletano","sequence":"first","affiliation":[{"name":"Department of Experimental Medicine \u201cSapienza\u201d University of Rome  Rome Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3881-095X","authenticated-orcid":false,"given":"S.","family":"Mattiucci","sequence":"additional","affiliation":[{"name":"Department of Public Health and Infectious Diseases Laboratory affiliated to Istituto Pasteur Italia \u2010 Fondazione Cenci Bolognetti University Hospital \u201cPoliclinico Umberto I\u201d \u201cSapienza\u201d University of Rome  Rome Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.","family":"Colantoni","sequence":"additional","affiliation":[{"name":"Department of Public Health and Infectious Diseases Laboratory affiliated to Istituto Pasteur Italia \u2010 Fondazione Cenci Bolognetti University Hospital \u201cPoliclinico Umberto I\u201d \u201cSapienza\u201d University of Rome  Rome Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"F.","family":"Battisti","sequence":"additional","affiliation":[{"name":"Department of Experimental Medicine \u201cSapienza\u201d University of Rome  Rome Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"I. 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