{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,3]],"date-time":"2025-11-03T09:16:53Z","timestamp":1762161413982,"version":"3.41.2"},"reference-count":43,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2018,7,8]],"date-time":"2018-07-08T00:00:00Z","timestamp":1531008000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Parasite Immunology"],"published-print":{"date-parts":[[2018,8]]},"abstract":"Summary<\/jats:title>Cutaneous leishmaniasis (CL<\/jats:styled-content>) treatment is based on therapy with Glucantime\u00ae<\/jats:sup>, yet, there are few laboratory methods to monitor its success. In this study, ex vivo and in vitro evaluations of peripheral blood monocytes were performed in a longitudinal study to characterize the impact of Glucantime\u00ae<\/jats:sup> on overall phenotypic\/functional features of these cells from CL<\/jats:styled-content> patients to identify predictive biomarkers for post\u2010therapeutic monitoring by flow cytometry. The ex vivo evaluation from CL<\/jats:styled-content> patients demonstrated a modulatory profile before treatment, with a decrease in TLR<\/jats:styled-content>\u20102, Fc\u03b3RII<\/jats:styled-content>, HLA<\/jats:styled-content>\u2010DR<\/jats:styled-content>, CD<\/jats:styled-content>86, IFN<\/jats:styled-content>\u2010\u03b3R, TNF<\/jats:styled-content>, IL<\/jats:styled-content>\u201012, NO<\/jats:styled-content>, and an increase in Fc\u03b3RIII<\/jats:styled-content> and IL<\/jats:styled-content>\u201010R. Conversely, treatment changes some of these biomarker expressions by decreasing Fc\u03b3RIII<\/jats:styled-content> and IL<\/jats:styled-content>\u201010R and increasing IFN<\/jats:styled-content>\u2010\u03b3R, IL<\/jats:styled-content>\u201012 and NO<\/jats:styled-content>. Moreover, an in vitro analysis of these patients showed a reduced phagocytic capacity of Leishmania braziliensis<\/jats:italic> and higher levels of IL<\/jats:styled-content>\u201010 and TGF<\/jats:styled-content>\u2010\u03b2 modulating functional profile. Regardless of the compromised L.\u00a0braziliensis<\/jats:italic> phagocytic capacity, treatment re\u2010established the production of IL<\/jats:styled-content>\u201012, IL<\/jats:styled-content>\u201010, TGF<\/jats:styled-content>\u2010\u03b2 and NO<\/jats:styled-content> at the basal level. Notably, monocytes from patients with early cicatrization showed enhanced Fc\u03b3RI<\/jats:styled-content> and Fc\u03b3RII<\/jats:styled-content> expressions and reduced IL<\/jats:styled-content>\u201010, which was further corroborated by a baseline fold change analysis. Finally, the logistic regression model emphasized the performance of Fc\u03b3RI<\/jats:styled-content>, Fc\u03b3RII<\/jats:styled-content> and IL<\/jats:styled-content>\u201010 as robust predictive biomarkers for post\u2010therapeutic cicatrization during cutaneous leishmaniasis.<\/jats:p>","DOI":"10.1111\/pim.12565","type":"journal-article","created":{"date-parts":[[2018,6,16]],"date-time":"2018-06-16T10:05:25Z","timestamp":1529143525000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Fc\u03b3\u2010RI<\/scp>, Fc\u03b3\u2010RII<\/scp> and IL<\/scp>\u201010 as predictive biomarkers for post\u2010therapeutic cicatrization time in monocytes from cutaneous leishmaniasis patients"],"prefix":"10.1111","volume":"40","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4983-2751","authenticated-orcid":false,"given":"M. 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G.","family":"Coelho\u2010dos\u2010Reis","sequence":"additional","affiliation":[{"name":"Grupo Integrado de Pesquisa em Biomarcadores Centro de Pesquisas Ren\u00e9 Rachou, Fiocruz Belo Horizonte Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M. A.","family":"Pascoal\u2010Xavier","sequence":"additional","affiliation":[{"name":"Pesquisa Cl\u00ednica e Pol\u00edticas P\u00fablicas em Doen\u00e7as Infecciosas e Parasit\u00e1rias Centro de Pesquisas Ren\u00e9 Rachou, Fiocruz Belo Horizonte Brazil"},{"name":"Departamento de Anatomia Patol\u00f3gica e Medicina Legal Faculdade de Medicina Universidade Federal de Minas Gerais Belo Horizonte Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H.","family":"Alves\u2010Costa","sequence":"additional","affiliation":[{"name":"Grupo Integrado de Pesquisa em Biomarcadores Centro de Pesquisas Ren\u00e9 Rachou, Fiocruz Belo Horizonte Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C. 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