{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,20]],"date-time":"2025-09-20T21:43:40Z","timestamp":1758404620843,"version":"3.41.2"},"reference-count":21,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2018,7,13]],"date-time":"2018-07-13T00:00:00Z","timestamp":1531440000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","award":["400063\/2016\u20106"],"award-info":[{"award-number":["400063\/2016\u20106"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Parasite Immunology"],"published-print":{"date-parts":[[2018,8]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>Eosinophils are traditionally associated with the immune response against helminth parasites. However, several studies have demonstrated that these cells have a role regarding protective immunity in leishmaniasis. Here, we examined the relationship between the presence of eosinophils and parasite load in biopsy samples from dogs, obtained through fine needle puncture and aspiration of lymph nodes. Histological slides containing biopsy material from lymph nodes of dogs with canine visceral leishmaniasis and healthy dogs were used to obtain baseline eosinophil counts. Subsequently, scrapings were taken from slides for DNA extraction and determination of parasite load, using real\u2010time PCR (qRT\u2010PCR). Additionally, production of nitric oxide (NO) and reactive oxygen species (ROS) levels by eosinophils in the peripheral blood of dogs with canine visceral leishmaniasis and healthy dogs was measured. The eosinophil percentage were higher in lymph nodes of infected group, and the parasite load showed a significant negative correlation with the eosinophil count. The production of NO and ROS by eosinophils in the peripheral blood was higher in the dogs with canine visceral leishmaniasis. All the results together suggest that eosinophils may participate in antileishmanial immunity in canine visceral leishmaniasis.<\/jats:p>","DOI":"10.1111\/pim.12567","type":"journal-article","created":{"date-parts":[[2018,6,19]],"date-time":"2018-06-19T19:08:50Z","timestamp":1529435330000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Eosinophilic inflammation in lymph nodes of dogs with visceral leishmaniasis"],"prefix":"10.1111","volume":"40","author":[{"given":"Sidnei Ferro","family":"Costa","sequence":"first","affiliation":[{"name":"Master's student of Animal Science School of Veterinary Medicine S\u00e3o Paulo State University (UNESP)  Ara\u00e7atuba S\u00e3o Paulo Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gabriel Franco","family":"Trivellato","sequence":"additional","affiliation":[{"name":"Undergraduate student of Animal Science School of Veterinary Medicine S\u00e3o Paulo State University (UNESP)  Ara\u00e7atuba S\u00e3o Paulo Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gabriela Torres","family":"Rebech","sequence":"additional","affiliation":[{"name":"Master's student of Animal Science School of Veterinary Medicine S\u00e3o Paulo State University (UNESP)  Ara\u00e7atuba S\u00e3o Paulo Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marilene","family":"Oliveira dos Santos Maciel","sequence":"additional","affiliation":[{"name":"Master's student of Animal Science School of Veterinary Medicine S\u00e3o Paulo State University (UNESP)  Ara\u00e7atuba S\u00e3o Paulo Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Larissa Martins","family":"Melo","sequence":"additional","affiliation":[{"name":"Doctoral student of Animal Science School of Veterinary Medicine S\u00e3o Paulo State University (UNESP)  Ara\u00e7atuba S\u00e3o Paulo Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria Cec\u00edlia Rui","family":"Luvizotto","sequence":"additional","affiliation":[{"name":"Department of Surgery and Animal Reproduction School of Veterinary Medicine S\u00e3o Paulo State University (UNESP)  Ara\u00e7atuba S\u00e3o Paulo Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0954-5045","authenticated-orcid":false,"given":"Val\u00e9ria Mar\u00e7al Felix","family":"de Lima","sequence":"additional","affiliation":[{"name":"Department of Surgery and Animal Reproduction School of Veterinary Medicine S\u00e3o Paulo State University (UNESP)  Ara\u00e7atuba S\u00e3o Paulo Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2018,7,13]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1590\/S1984-29612014008"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00436-010-2247-6"},{"key":"e_1_2_8_4_1","unstructured":"World Health Organization (WHO).Global Health Observatory (GHO) data. 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