{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T12:32:28Z","timestamp":1753878748917,"version":"3.41.2"},"reference-count":35,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2018,9,21]],"date-time":"2018-09-21T00:00:00Z","timestamp":1537488000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100007129","name":"Natural Science Foundation of Shandong Province","doi-asserted-by":"publisher","award":["ZR2013HM033"],"award-info":[{"award-number":["ZR2013HM033"]}],"id":[{"id":"10.13039\/501100007129","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Parasite Immunology"],"published-print":{"date-parts":[[2018,10]]},"abstract":"<jats:title>Summary<\/jats:title><jats:sec><jats:title>Aims<\/jats:title><jats:p><jats:italic>Toxoplasma gondii<\/jats:italic> is an intracellular parasite that causes a global epidemic parasitic disease. Studies using <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> vaccines for the control of toxoplasmosis have made considerable progress. <jats:styled-content style=\"fixed-case\">ROP<\/jats:styled-content> proteins were proven to be excellent candidates for <jats:italic>T.\u00a0gondii <\/jats:italic><jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> vaccine development.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods and Results<\/jats:title><jats:p>Here, a <jats:styled-content style=\"fixed-case\">ROP<\/jats:styled-content>29 <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> vaccine was successfully produced and injected into mice in combination with R848 to evaluate its ability to provide protection against <jats:italic>T.\u00a0gondii<\/jats:italic> challenge. Compared with other mice, the mice injected with R848\/<jats:styled-content style=\"fixed-case\">pROP<\/jats:styled-content>29 produced higher levels of IgG, IgG2a, interleukin (<jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>)\u201012, and interferon gamma (<jats:styled-content style=\"fixed-case\">IFN<\/jats:styled-content>\u2010\u03b3). Moreover, after a challenge of 20 <jats:italic>T.\u00a0gondii<\/jats:italic> cysts, the number of brain cysts was lower in the R848\/<jats:styled-content style=\"fixed-case\">pROP<\/jats:styled-content>29\u2010immunized mice than in the other experimental mice.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>R848 could improve the productions of <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u201012 and <jats:styled-content style=\"fixed-case\">IFN<\/jats:styled-content>\u2010\u03b3, thus enhancing the immune responses stimulated by the <jats:styled-content style=\"fixed-case\">pROP<\/jats:styled-content>29 <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> vaccine.<\/jats:p><\/jats:sec>","DOI":"10.1111\/pim.12578","type":"journal-article","created":{"date-parts":[[2018,8,9]],"date-time":"2018-08-09T07:18:10Z","timestamp":1533799090000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["<scp>DNA<\/scp> vaccine <scp>ROP<\/scp>29 from <i>Toxoplasma gondii<\/i> containing R848 enhances protective immunity in mice"],"prefix":"10.1111","volume":"40","author":[{"given":"Gang","family":"Lu","sequence":"first","affiliation":[{"name":"Institute of Pathogen Biology Taishan Medical College  Taian Shandong China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jian","family":"Zhou","sequence":"additional","affiliation":[{"name":"Department of Orthopedics The Second Xiangya Hospital Central South University  Changsha Hunan China"},{"name":"Department of Sports Medicine Research Center Central South University  Changsha 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