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Here, we investigated whether such antigenic peptides were found in proteins that were already known as vaccine candidates and excretome\/secretome proteins for <jats:italic>Onchocerca volvulus<\/jats:italic> This approach led to the identification of 71 immunoreactive stretches in 46 proteins. A deep\u2010dive into the immunoreactivity profiles of eight vaccine candidates that were chosen as most promising candidates for further development (<jats:italic>Ov<\/jats:italic>\u2010<jats:styled-content style=\"fixed-case\">CPI<\/jats:styled-content>\u20102, <jats:italic>Ov<\/jats:italic>\u2010<jats:styled-content style=\"fixed-case\">ALT<\/jats:styled-content>\u20101, <jats:italic>Ov<\/jats:italic>\u2010<jats:styled-content style=\"fixed-case\">RAL<\/jats:styled-content>\u20102, <jats:italic>Ov<\/jats:italic>\u2010<jats:styled-content style=\"fixed-case\">ASP<\/jats:styled-content>\u20101, <jats:italic>Ov<\/jats:italic>\u2010103, <jats:italic>Ov<\/jats:italic>\u2010<jats:styled-content style=\"fixed-case\">RBP<\/jats:styled-content>\u20101, <jats:italic>Ov<\/jats:italic>\u2010<jats:styled-content style=\"fixed-case\">CHI<\/jats:styled-content>\u20101, and <jats:italic>Ov<\/jats:italic>\u2010B20), resulted in the identification of a poly\u2010glutamine stretch in <jats:italic>Ov<\/jats:italic>\u2010<jats:styled-content style=\"fixed-case\">RAL<\/jats:styled-content>\u20102 that has properties for use as a serodiagnostic marker for <jats:italic>O.\u00a0volvulus<\/jats:italic> infection. A peptide <jats:styled-content style=\"fixed-case\">ELISA<\/jats:styled-content> was developed, and the performance of this assay was evaluated. Based on this assessment, it was found that this assay has a sensitivity of 75.0% [95% <jats:styled-content style=\"fixed-case\">CI<\/jats:styled-content>: 64.9%\u201083.5%] and a specificity of 98.5% [95% <jats:styled-content style=\"fixed-case\">CI<\/jats:styled-content>: 94.6%\u201099.8%]. Furthermore, 8.7% reactivity in Asian parasite\u2010infected individuals (8 out of 92) was observed. Besides this identification of a linear epitope marker, the information on the presence of linear epitopes in vaccine candidate proteins might be useful in the study of vaccines for river blindness.<\/jats:p>","DOI":"10.1111\/pim.12587","type":"journal-article","created":{"date-parts":[[2018,9,6]],"date-time":"2018-09-06T14:53:20Z","timestamp":1536245600000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Linear epitopes in <i>Onchocerca volvulus<\/i> vaccine candidate proteins and excretory\u2010secretory proteins"],"prefix":"10.1111","volume":"40","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0638-1313","authenticated-orcid":false,"given":"Ole","family":"Lagatie","sequence":"first","affiliation":[{"name":"Janssen Diagnostics Janssen R&amp;D  Beerse Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ann","family":"Verheyen","sequence":"additional","affiliation":[{"name":"Janssen Diagnostics Janssen R&amp;D  Beerse Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bieke","family":"Van Dorst","sequence":"additional","affiliation":[{"name":"Janssen Diagnostics Janssen R&amp;D  Beerse Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Linda","family":"Batsa Debrah","sequence":"additional","affiliation":[{"name":"Department of Clinical Microbiology School of Medical Sciences Kwame Nkrumah University of Science and Technology  Kumasi Ghana"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alex","family":"Debrah","sequence":"additional","affiliation":[{"name":"Faculty of Allied Health Sciences Kwame Nkrumah University of Science and Technology  Kumasi Ghana"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lieven J.","family":"Stuyver","sequence":"additional","affiliation":[{"name":"Janssen Diagnostics Janssen R&amp;D  Beerse Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2018,10,8]]},"reference":[{"doi-asserted-by":"publisher","key":"e_1_2_7_2_1","DOI":"10.1016\/S0140-6736(14)60875-8"},{"doi-asserted-by":"publisher","key":"e_1_2_7_3_1","DOI":"10.1172\/JCI34261"},{"unstructured":"WHO.Report of the Tenth Meeting of the WHO Strategic and Technical Advisory Group for Neglected Tropical Diseases. 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