{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,3]],"date-time":"2026-04-03T21:46:30Z","timestamp":1775252790575,"version":"3.50.1"},"reference-count":45,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2018,11,15]],"date-time":"2018-11-15T00:00:00Z","timestamp":1542240000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100012021","name":"Iran University of Medical Sciences","doi-asserted-by":"publisher","award":["27899"],"award-info":[{"award-number":["27899"]}],"id":[{"id":"10.13039\/100012021","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Parasite Immunology"],"published-print":{"date-parts":[[2019,1]]},"abstract":"<jats:title>Summary<\/jats:title>\n                  <jats:p>\n                    Dirofilariasis is a zoonotic global vector\u2010borne disease caused by\n                    <jats:italic>Dirofilaria immitis<\/jats:italic>\n                    . The present study focuses on the somatic and excretory\/secretory (E\/S) proteins released from adult\n                    <jats:italic>D.\u00a0immitis<\/jats:italic>\n                    . We aimed to fractionate and identify adult\n                    <jats:italic>D.\u00a0immitis<\/jats:italic>\n                    immunoreactive proteins. Somatic and E\/S extracts were immunoblotted to identify the immunoreactive proteins. In the current study, we used matrix\u2010assisted laser desorption\/ionization mass spectrometry (MALDI\u2010TOF\/MS) to characterize the immunogenic proteins. Additionally, we used fast protein liquid chromatography (FPLC) to fractionate and evaluate the immunogenicity of the\n                    <jats:italic>D.\u00a0immitis<\/jats:italic>\n                    secretome. The most immunoreactive proteins were between 10 and 48\u00a0kDa. Six proteins including polyprotein antigen, P22u, pepsin inhibitor Dit33, neutrophil chemotactic factor (DiNCF) precursor, glyceraldehyde\u20103\u2010phosphate dehydrogenase (GAPDH) and heat\u2010shock protein 70 (HSP70) were found in both somatic and E\/S extracts. Eluting the FPLC column with NaCl resolved two peaks in which the immunoreactivities of the purified proteins were conserved. Characterization of these proteins could provide a novel perspective for understanding the pathogenesis and diagnosing of this disease.\n                  <\/jats:p>","DOI":"10.1111\/pim.12598","type":"journal-article","created":{"date-parts":[[2018,10,17]],"date-time":"2018-10-17T21:11:26Z","timestamp":1539810686000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Application of\n                    <i>Dirofilaria immitis<\/i>\n                    immunoreactive proteins in serodiagnosis"],"prefix":"10.1111","volume":"41","author":[{"given":"Majid","family":"Khanmohammadi","sequence":"first","affiliation":[{"name":"Department of Parasitology and Mycology School of Medicine Iran University of Medical Sciences Tehran Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1842-2952","authenticated-orcid":false,"given":"Reza","family":"Falak","sequence":"additional","affiliation":[{"name":"Immunology Research Center Iran University of Medical Sciences Tehran Iran"},{"name":"Department of Immunology School of Medicine Iran University of Medical Sciences Tehran Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ahmad Reza","family":"Meamar","sequence":"additional","affiliation":[{"name":"Department of Parasitology and Mycology School of Medicine Iran University of Medical Sciences Tehran Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elham","family":"Razmjou","sequence":"additional","affiliation":[{"name":"Department of Parasitology and Mycology School of Medicine Iran University of Medical Sciences Tehran Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kobra","family":"Mokhtarian","sequence":"additional","affiliation":[{"name":"Medical Plant Research Center Basic Health Sciences Institute Shahrekord University of Medical Sciences Shahrekord Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mehdi","family":"Arshadi","sequence":"additional","affiliation":[{"name":"Department of Parasitology and Mycology School of Medicine Iran University of Medical Sciences Tehran Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nasrin","family":"Shayanfar","sequence":"additional","affiliation":[{"name":"Department of pathology School of Medicine Iran University of Medical Sciences Tehran Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lame","family":"Akhlaghi","sequence":"additional","affiliation":[{"name":"Department of Parasitology and Mycology School of Medicine Iran University of Medical Sciences Tehran 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