{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,31]],"date-time":"2026-07-31T22:29:39Z","timestamp":1785536979707,"version":"3.56.0"},"reference-count":38,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2019,10,18]],"date-time":"2019-10-18T00:00:00Z","timestamp":1571356800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Parasite Immunology"],"published-print":{"date-parts":[[2019,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:italic>Anoplocephala perfoliata<\/jats:italic> is the commonest equine tapeworm, the\u00a0adult parasites are attached in groups close to the ileocaecal valve causing marked inflammatory pathology. This work aimed to characterize the nature of the in vivo mucosal immune response to <jats:italic>A\u00a0perfoliata<\/jats:italic>, and to investigate the role of <jats:italic>A\u00a0perfoliata<\/jats:italic> excretory\u2010secretory components in modulating in vitro immune responses. Real\u2010time PCR detected elevation of IL13 and TGF\u03b2 transcription in early\u2010stage <jats:italic>A\u00a0perfoliata<\/jats:italic> infection. In late\u2010stage infection, IL\u201013, IL4 and Ifn transcripts were reduced while the regulatory cytokines, TGF\u03b2, IL10 and the transcription factor FOXP3 were increased in tissue close to the site of <jats:italic>A\u00a0perfoliata<\/jats:italic> attachment; indicating downregulation of T\u2010cell responses to <jats:italic>A\u00a0perfoliata<\/jats:italic>. In vitro, <jats:italic>A\u00a0perfoliata<\/jats:italic> excretory\u2010secretory products induced apoptosis of the Jurkat T\u2010cell line and premature cell death of ConA stimulated equine peripheral blood leucocytes. Analysis of cytokine transcription patterns in the leucocyte cultures showed a marked inhibition of IL\u20101 and IL\u20102 suggesting that a lack of T\u2010cell growth factor transcription underlies the mechanism of the induced equine T\u2010cell death. These preliminary findings suggest <jats:italic>A\u00a0perfoliata<\/jats:italic> may have the ability to down\u2010regulate host T\u2010cell responses.<\/jats:p>","DOI":"10.1111\/pim.12667","type":"journal-article","created":{"date-parts":[[2019,8,23]],"date-time":"2019-08-23T17:19:12Z","timestamp":1566580752000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Analysis of caecal mucosal inflammation and immune modulation during <i>Anoplocephala perfoliata<\/i> infection of horses"],"prefix":"10.1111","volume":"41","author":[{"given":"April L.","family":"Lawson","sequence":"first","affiliation":[{"name":"School of Clinical Veterinary Sciences University of Bristol Bristol UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Charles E.","family":"Pittaway","sequence":"additional","affiliation":[{"name":"School of Clinical Veterinary Sciences University of Bristol Bristol UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Richard M.","family":"Sparrow","sequence":"additional","affiliation":[{"name":"School of Clinical Veterinary Sciences University of Bristol Bristol UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emily C.","family":"Balkwill","sequence":"additional","affiliation":[{"name":"School of Clinical Veterinary Sciences University of Bristol Bristol UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gerald C.","family":"Coles","sequence":"additional","affiliation":[{"name":"School of Clinical Veterinary Sciences University of Bristol Bristol UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alice","family":"Tilley","sequence":"additional","affiliation":[{"name":"School of Clinical Veterinary Sciences University of Bristol Bristol UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0557-5914","authenticated-orcid":false,"given":"A. Douglas","family":"Wilson","sequence":"additional","affiliation":[{"name":"School of Clinical Veterinary Sciences University of Bristol Bristol UK"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2019,10,18]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1017\/S0031182004007127"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00436-012-3150-0"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.2478\/s11687-009-0002-2"},{"key":"e_1_2_8_5_1","first-page":"319","article-title":"Prevalence of gastrointestinal parasites in working horses","volume":"13","author":"Tavassoli M","year":"2010","journal-title":"Pol J Vet Sci"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00436-014-4279-9"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetpar.2005.03.040"},{"key":"e_1_2_8_8_1","first-page":"312","article-title":"A study on the seasonal epidemiology of Anoplocephala spp.\u2010infection in horses and the appropriate treatment using a praziquantel gel (Droncit (R) 9% oral gel)","volume":"119","author":"Roelfstra L","year":"2006","journal-title":"Berl Munch Tierarztl Wochenschr"},{"key":"e_1_2_8_9_1","first-page":"32","article-title":"Prevalence and risk factor analysis of equine infestation with gastrointestinal parasites in Israel","volume":"70","author":"Levy ST","year":"2015","journal-title":"Isr J Vet Med"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetpar.2012.05.022"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1136\/vr.134.20.515"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1136\/vr.123.22.562"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0304-4017(97)00123-4"},{"key":"e_1_2_8_14_1","first-page":"752","article-title":"Intussusception associated with Anoplocephala perfoliata infection in 5 horses","volume":"180","author":"Barclay WP","year":"1982","journal-title":"J Am Vet Med Assoc"},{"key":"e_1_2_8_15_1","first-page":"804","article-title":"Donawick WJ Cecal perforation and peritonitis associated with Anoplocephala perfoliata Infection in 3 Horses","volume":"183","author":"Beroza GA","year":"1983","journal-title":"J Am Vet Med Assoc"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.2746\/042516402776117773"},{"key":"e_1_2_8_17_1","first-page":"26","article-title":"Equine colic caused by tape worms \u2010 5 clinical case reports","volume":"88","author":"Mezerova J","year":"2007","journal-title":"Prakt Tierarzt"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.2042-3306.1993.tb02948.x"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.2042-3306.1998.tb04487.x"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetpar.2013.07.020"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetpar.2010.10.041"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1136\/vr.132.8.179"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetpar.2013.10.005"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/0304-4017(94)00755-2"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-3024.1996.d01-18.x"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13567-016-0339-8"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1186\/gb-2002-3-7-research0034"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1300911"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2015.08.020"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00281-016-0606-9"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.it.2011.01.006"},{"key":"e_1_2_8_32_1","doi-asserted-by":"publisher","DOI":"10.1038\/mi.2016.56"},{"key":"e_1_2_8_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0304-4017(02)00119-X"},{"key":"e_1_2_8_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetimm.2008.10.316"},{"key":"e_1_2_8_35_1","doi-asserted-by":"publisher","DOI":"10.1111\/pim.12289"},{"key":"e_1_2_8_36_1","doi-asserted-by":"publisher","DOI":"10.1017\/S0031182009990886"},{"key":"e_1_2_8_37_1","doi-asserted-by":"publisher","DOI":"10.1017\/S0031182008005210"},{"key":"e_1_2_8_38_1","doi-asserted-by":"publisher","DOI":"10.1111\/pim.12327"},{"key":"e_1_2_8_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(91)90124-H"}],"container-title":["Parasite Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fpim.12667","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/pim.12667","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/pim.12667","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/pim.12667","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,8]],"date-time":"2023-09-08T14:11:45Z","timestamp":1694182305000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/pim.12667"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,10,18]]},"references-count":38,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2019,11]]}},"alternative-id":["10.1111\/pim.12667"],"URL":"https:\/\/doi.org\/10.1111\/pim.12667","archive":["Portico"],"relation":{},"ISSN":["0141-9838","1365-3024"],"issn-type":[{"value":"0141-9838","type":"print"},{"value":"1365-3024","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,10,18]]},"assertion":[{"value":"2019-03-22","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-08-16","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-10-18","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e12667"}}