{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,19]],"date-time":"2026-08-19T18:26:13Z","timestamp":1787163973758,"version":"build-2736575974"},"reference-count":31,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2019,9,30]],"date-time":"2019-09-30T00:00:00Z","timestamp":1569801600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100008915","name":"Department of Transport and Main Roads, Queensland Government","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100008915","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001408","name":"Department of Scientific and Industrial Research, Ministry of Science and Technology","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001408","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000925","name":"National Health and Medical Research Council","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000925","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000009","name":"Foundation for the National Institutes of Health","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000009","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Parasite Immunology"],"published-print":{"date-parts":[[2019,11]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    CD8\n                    <jats:sup>+<\/jats:sup>\n                    T\u2010cell function is compromised in chronic diseases such as visceral leishmaniasis (VL). However, little is known about the changes in gene expression that cause CD8+ T\u2010cell dysfunction during VL. We used targeted transcriptional profiling of peripheral blood CD8\n                    <jats:sup>+<\/jats:sup>\n                    T cells from VL patients pre\u2010 and post\u2010anti\u2010parasitic drug treatment, and compared them with the same cell population from healthy endemic controls to assess their activation, differentiation and functional status during disease. We found a predominance of downregulated immune genes in CD8\n                    <jats:sup>+<\/jats:sup>\n                    T cells from VL patients. However, genes encoding several notable immune checkpoint molecules, including LAG\u20103, TIM\u20103 and CTLA\u20104, cytolytic molecules, such as granzymes A, B and H and perforin, as well as SOCS3, STAT1, JAK2 and JAK3 cytokine signalling genes were found to be increasingly expressed by VL patient CD8\n                    <jats:sup>+<\/jats:sup>\n                    T cells. Additional studies confirmed increased expression of the inhibitory receptors LAG3 and TIM3 on VL patient CD8\n                    <jats:sup>+<\/jats:sup>\n                    T cells, thereby identifying these molecules as potential targets to improve antigen\u2010specific CD8\n                    <jats:sup>+<\/jats:sup>\n                    T\u2010cell responses during disease.\n                  <\/jats:p>","DOI":"10.1111\/pim.12669","type":"journal-article","created":{"date-parts":[[2019,9,8]],"date-time":"2019-09-08T09:50:22Z","timestamp":1567936222000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":21,"title":["A molecular signature for CD8\n                    <sup>+<\/sup>\n                    T cells from visceral leishmaniasis patients"],"prefix":"10.1111","volume":"41","author":[{"given":"Bhawana","family":"Singh","sequence":"first","affiliation":[{"name":"Department of Medicine Institute of Medical Sciences Banaras Hindu University Varanasi India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3268-852X","authenticated-orcid":false,"given":"Shashi","family":"Bhushan Chauhan","sequence":"additional","affiliation":[{"name":"Department of Medicine Institute of Medical Sciences Banaras Hindu University Varanasi India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rajiv","family":"Kumar","sequence":"additional","affiliation":[{"name":"Centre of Experimental Medicine &amp; Surgery Institute of Medical Sciences Banaras Hindu University Varanasi India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Siddharth Sankar","family":"Singh","sequence":"additional","affiliation":[{"name":"Department of Medicine Institute of Medical Sciences Banaras Hindu University Varanasi India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Susanna","family":"Ng","sequence":"additional","affiliation":[{"name":"QIMR 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Differential T cell and lymphokine roles in initial versus established immunity","volume":"148","author":"Murray HW","year":"1992","journal-title":"J Immunol"}],"container-title":["Parasite Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/pim.12669","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/pim.12669","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/pim.12669","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,8]],"date-time":"2023-09-08T10:11:51Z","timestamp":1694167911000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/pim.12669"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,9,30]]},"references-count":31,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2019,11]]}},"alternative-id":["10.1111\/pim.12669"],"URL":"https:\/\/doi.org\/10.1111\/pim.12669","archive":["Portico"],"relation":{},"ISSN":["0141-9838","1365-3024"],"issn-type":[{"value":"0141-9838","type":"print"},{"value":"1365-3024","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,9,30]]},"assertion":[{"value":"2019-06-24","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-08-31","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-09-30","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e12669"}}