{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,22]],"date-time":"2026-04-22T14:17:06Z","timestamp":1776867426242,"version":"3.51.2"},"reference-count":34,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2019,10,13]],"date-time":"2019-10-13T00:00:00Z","timestamp":1570924800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Parasite Immunology"],"published-print":{"date-parts":[[2019,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Cystic echinococcosis (CE) can be diagnosed by means of several serological approaches, but their results vary among laboratories due to the molecular characteristics of the reference antigens used. Thus, this study aimed to address both the relevance of an EGPE cell line previously obtained from <jats:italic>Echinococcus granulosus<\/jats:italic> protoscoleces G1 and the complexity of the immune response by using two different in vitro growth stages as separate sources of parasite antigens. The serum reactivity was investigated by western blotting (WB) in 21 CE patients from an endemic area in a matched case\u2010control design and also in seven experimentally infected sheep and five healthy control sheep. EGPE\u2010antigen\u2010human serum sensitivity by WB was higher than that of hydatid fluid (HF) WB, ELISA and DD5 (<jats:italic>P<\/jats:italic>\u00a0&lt;\u00a0.05, Chi\u2010square test). EGPE protein extract was immunogenic in mice and hyperimmune plasma reacted with HF proteins, and AgB2 expression was detected by molecular analysis. Proteins of 37 to 60\u00a0kDa were recognized by 95.24% of the CE patients\u2019 sera but, with poor specificity. Statistically significant differences were found between serum protein extract recognition at 7 and 20\u00a0days of cell growth. The EGPE cell line is a laboratory source of antigens for improvement of CE serological diagnosis.<\/jats:p>","DOI":"10.1111\/pim.12674","type":"journal-article","created":{"date-parts":[[2019,9,27]],"date-time":"2019-09-27T02:11:50Z","timestamp":1569550310000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Detection of <i>Echinococcus granulosus<\/i> sensu<i> lato<\/i> infection by using extracts derived from a protoscoleces G1 cell line"],"prefix":"10.1111","volume":"41","author":[{"given":"Andrea","family":"Maglioco","sequence":"first","affiliation":[{"name":"Centro de Altos Estudios en Ciencias Humanas y de la Salud (CAECIHS) Universidad Abierta Interamericana (UAI) Buenos Aires Argentina"},{"name":"Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas (CONICET) Buenos Aires 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