{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,17]],"date-time":"2026-09-17T12:04:19Z","timestamp":1789646659471,"version":"4.0.0"},"reference-count":13,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2020,1,27]],"date-time":"2020-01-27T00:00:00Z","timestamp":1580083200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100010426","name":"UGC-DAE\u00a0Consortium for Scientific Research, University Grants Commission","doi-asserted-by":"publisher","award":["BT\/In\/Infect\u2010eRA\/33\/BS\/2016\u201017"],"award-info":[{"award-number":["BT\/In\/Infect\u2010eRA\/33\/BS\/2016\u201017"]}],"id":[{"id":"10.13039\/501100010426","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Parasite Immunology"],"published-print":{"date-parts":[[2020,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Aims<\/jats:title><jats:p>This study aimed at evaluating the DNA vaccination efficacy of <jats:italic>Leishmania major<\/jats:italic>\u2010derived <jats:italic>MAPK10<\/jats:italic> against <jats:italic>Leishmania donovani<\/jats:italic> infection.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods and Results<\/jats:title><jats:p>MAPK10 is one of the 15 mitogen\u2010activated protein kinases (MAPKs) of <jats:italic>Leishmania major<\/jats:italic>. Herein, we expressed the gene through a mammalian vector and tested whether priming with this gene would offer protection against <jats:italic>L\u00a0donovani<\/jats:italic> infection. We report that LmjMAPK10 DNA vaccination using a mammalian expression vector significantly reduces the parasite burden. The protection is accompanied by host\u2010protective T\u2010cell functions, T<jats:sub>H<\/jats:sub>1\u2010type cytokines and elevated leishmanial antigen\u2010specific IgG2a isotype response. T\u2010cell response to the <jats:italic>L\u00a0donovani<\/jats:italic>\/challenge infection is associated with increase in IL\u201012 and IFN\u2010\u03b3, but reduced IL\u201010 and IL\u20104 production.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>LmjMAPK10 is cross\u2010protective against <jats:italic>L\u00a0donovani<\/jats:italic> infection.<\/jats:p><\/jats:sec>","DOI":"10.1111\/pim.12687","type":"journal-article","created":{"date-parts":[[2019,11,26]],"date-time":"2019-11-26T17:24:06Z","timestamp":1574789046000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["LmjMAPK10 offers protection against <i>Leishmania donovani<\/i> infection"],"prefix":"10.1111","volume":"42","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3139-114X","authenticated-orcid":false,"given":"Sunil","family":"Kumar","sequence":"first","affiliation":[{"name":"National Centre for Cell Science Pune India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5460-1683","authenticated-orcid":false,"given":"Shubhranshu","family":"Zutshi","sequence":"additional","affiliation":[{"name":"National Centre for Cell Science Pune India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ashok","family":"Patidar","sequence":"additional","affiliation":[{"name":"National Centre for Cell Science Pune India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Neelam","family":"Bodhale","sequence":"additional","affiliation":[{"name":"National Centre for Cell Science Pune India"},{"name":"JBNSTS Kolkata India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Somenath","family":"Roy","sequence":"additional","affiliation":[{"name":"Department of Human Physiology with Community Health Vidyasagar University Midnapore India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arup","family":"Sarkar","sequence":"additional","affiliation":[{"name":"Trident Academy of Creative Technology Bhubaneswar India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5833-7912","authenticated-orcid":false,"given":"Bhaskar","family":"Saha","sequence":"additional","affiliation":[{"name":"National Centre for Cell Science Pune India"},{"name":"Trident Academy of Creative Technology Bhubaneswar India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,1,27]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1080\/14760584.2018.1459191"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2011.04.030"},{"key":"e_1_2_10_4_1","doi-asserted-by":"crossref","first-page":"1182","DOI":"10.4049\/jimmunol.148.4.1182","article-title":"IL\u20104 induces a Th2 response in Leishmania major\u2010infected mice","volume":"148","author":"Chatelain R","year":"1992","journal-title":"J Immunol"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/nri.2016.72"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.166.2.1141"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.micinf.2017.09.011"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.174.11.7160"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm1045"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1400672"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.177.1.22"},{"key":"e_1_2_10_12_1","first-page":"238","article-title":"Immune response to Leishmania infection","volume":"119","author":"Awasthi A","year":"2004","journal-title":"Indian J Med Res"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cyto.2017.10.005"},{"key":"e_1_2_10_14_1","first-page":"767","article-title":"Evaluation of toxicity & therapeutic efficacy of a new liposomal formulation of amphotericin B in a mouse model","volume":"137","author":"Mishra J","year":"2013","journal-title":"Indian J Med Res"}],"container-title":["Parasite Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/pim.12687","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/pim.12687","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/pim.12687","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,7]],"date-time":"2023-09-07T22:00:21Z","timestamp":1694124021000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/pim.12687"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,1,27]]},"references-count":13,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2020,2]]}},"alternative-id":["10.1111\/pim.12687"],"URL":"https:\/\/doi.org\/10.1111\/pim.12687","archive":["Portico"],"relation":{},"ISSN":["0141-9838","1365-3024"],"issn-type":[{"value":"0141-9838","type":"print"},{"value":"1365-3024","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,1,27]]},"assertion":[{"value":"2019-07-17","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-11-18","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-01-27","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e12687"}}