{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,6]],"date-time":"2026-09-06T04:55:47Z","timestamp":1788670547550,"version":"build-2803163510"},"reference-count":29,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2020,2,7]],"date-time":"2020-02-07T00:00:00Z","timestamp":1581033600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003196","name":"Ministero della Salute","doi-asserted-by":"publisher","award":["GR\u20102016\u201002361497"],"award-info":[{"award-number":["GR\u20102016\u201002361497"]}],"id":[{"id":"10.13039\/501100003196","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003196","name":"Ministero della Salute","doi-asserted-by":"publisher","award":["Ricerca corrente, linea 1"],"award-info":[{"award-number":["Ricerca corrente, linea 1"]}],"id":[{"id":"10.13039\/501100003196","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Parasite Immunology"],"published-print":{"date-parts":[[2020,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The diagnosis of cystic echinococcosis (CE) is based on imaging, while serology is a complementary test of particular use when imaging is inconclusive. Serology has several limitations. Among them, false\u2010positive results are often obtained in subjects with alveolar echinococcosis (AE), rendering difficult the differential diagnosis. We set up an immune assay based on IL\u20104\u2010specific production after stimulating whole blood with an antigen B (AgB)\u2010enriched fraction from <jats:italic>E granulosus<\/jats:italic> that associates with CE and CE cysts in active stage. We aimed to evaluate potential cross\u2010reactivity of this test using samples from patients with AE. Twelve patients with AE were recruited; IL\u20104 levels ranged from 0 to 0.07\u00a0pg\/mL. Based on the previously identified cut\u2010off of 0.39\u00a0pg\/mL using samples from patients with CE, none of samples from AE patients scored positive. In contrast, almost 80% of samples from AE patients scored positive in serology tests based on different <jats:italic>E granulosus<\/jats:italic>\u2010derived antigenic preparations. Our preliminary data show that this experimental whole\u2010blood assay has no cross\u2010reactivity in our cohort of patients with AE, in turn indicating a high specificity of the assay for CE diagnosis. This result supports further work towards the development of improved diagnostic tests for CE.<\/jats:p>","DOI":"10.1111\/pim.12695","type":"journal-article","created":{"date-parts":[[2019,12,30]],"date-time":"2019-12-30T02:36:25Z","timestamp":1577673385000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Species specificity preliminary evaluation of an IL\u20104\u2010based test for the differential diagnosis of human echinococcosis"],"prefix":"10.1111","volume":"42","author":[{"given":"Linda","family":"Petrone","sequence":"first","affiliation":[{"name":"Translational Research Unit Department of Epidemiology and Preclinical Research National Institute for Infectious Diseases Lazzaro Spallanzani\u2013IRCCS Rome Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Werner C.","family":"Albrich","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases and Hospital Epidemiology 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