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The analysis revealed that the set of epitopes was presented by at least 54 diverse MHC class\u2010II alleles. Based on in silico screening, followed by molecular dynamics simulation, population coverage analysis, and HLA cross\u2010presentation ability, five best epitopes were evaluated. PBMCs isolated from treated VL subjects, when stimulated with synthetic peptide alone or as a cocktail of peptides, triggered a secretory IFN\u2010\u03b3, but not the IL\u201010 level. Support in this notion came from intracellular cytokine level with a considerable up\u2010regulated IFN\u2010\u03b3 produced by CD4<jats:sup>+<\/jats:sup> T cells. Also, the enhanced IFN\u2010\u03b3 seemed to be augmented with the activation of macrophages with prominent IL\u201012 production. Therefore, it can be concluded that the screened MHC class\u2010II\u2013restricted epitope hotspots derived from <jats:italic>Leishmania<\/jats:italic> ODC can trigger CD4<jats:sup>+<\/jats:sup> T cells, which can skew macrophage functions towards protection. However, a detailed analysis can explore its potentiality as a vaccine candidate.<\/jats:p>","DOI":"10.1111\/pim.12699","type":"journal-article","created":{"date-parts":[[2020,1,24]],"date-time":"2020-01-24T13:23:01Z","timestamp":1579872181000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["<i>Retracted:<\/i> Evaluating the immunomodulatory responses of <i>Ld<\/i>ODC\u2010derived MHC Class\u2010II restricted peptides against VL"],"prefix":"10.1111","volume":"42","author":[{"given":"RajKishor","family":"Pandey","sequence":"first","affiliation":[{"name":"Department of Biotechnology National Institute of Pharmaceutical Education &amp; Research Hajipur India"},{"name":"Division of Immunology Rajendra Memorial Research Institute of Medical Sciences Patna India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Manas Ranjan","family":"Dikhit","sequence":"additional","affiliation":[{"name":"Department of Biomedical Informatics Rajendra Memorial Research Institute of Medical Sciences Patna India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3331-2989","authenticated-orcid":false,"given":"Avinash","family":"Kumar","sequence":"additional","affiliation":[{"name":"Department of Biotechnology National Institute of Pharmaceutical Education &amp; 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