{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,2]],"date-time":"2026-05-02T06:25:46Z","timestamp":1777703146597,"version":"3.51.4"},"reference-count":26,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2020,3,20]],"date-time":"2020-03-20T00:00:00Z","timestamp":1584662400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Parasite Immunology"],"published-print":{"date-parts":[[2020,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Aims<\/jats:title><jats:p>The role of the immune response to cyathostomin infections in horses remains unknown. Intestinal goblet cell hyperplasia has previously been noted as a component in cyathostomin infection; however, the function is unclear. The goal of this study was to evaluate the local and systemic gene expression to cyathostomin infections following larvicidal treatment and explore their relation to goblet cells.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods and Results<\/jats:title><jats:p>Thirty\u2010six ponies with naturally acquired cyathostomin infections were randomly allocated into three groups: fenbendazole\u2010treated (10\u00a0mg\/kg PO 5\u00a0days), moxidectin\u2010treated (0.4\u00a0mg\/kg PO once) and untreated control. Whole blood from all horses was collected weekly, and tissue samples from the large intestine collected during necropsy at 2 and 5\u00a0weeks post\u2010treatment (WPT). Gene expression of interleukin (IL)\u20104, IL\u20105, IL\u20106, IL\u201010, IL\u201013, IL\u201017A, IL\u201022, IFN\u2010\u03b3, resistin\u2010like molecule beta (RELM\u2010\u03b2), Mucin 2 (MUC2) and tumour necrosis factor (TNF)\u2010\u03b1 was measured using qRT\u2010PCR. There were statistically significant linear correlations between luminal worm burdens and MUC2 (<jats:italic>r<\/jats:italic>\u00a0=\u00a0\u2212.2358) and RELM\u2010\u03b2 (<jats:italic>r<\/jats:italic>\u00a0=\u00a0\u2212.2261).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>This suggests an active role of immune system post\u2010treatment in parasite expulsion, specifically in goblet cells, and that the organs respond differently to treatment and the larvae themselves. This may have implications in the disease process and treatment.<\/jats:p><\/jats:sec>","DOI":"10.1111\/pim.12709","type":"journal-article","created":{"date-parts":[[2020,3,7]],"date-time":"2020-03-07T21:56:16Z","timestamp":1583618176000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Cytokine and goblet cell gene expression in equine cyathostomin infection and larvicidal anthelmintic therapy"],"prefix":"10.1111","volume":"42","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1063-5723","authenticated-orcid":false,"given":"Ashley E.","family":"Steuer","sequence":"first","affiliation":[{"name":"Maxwell H. Gluck Equine Research Center Department of Veterinary Science University of Kentucky Lexington KY USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"John C.","family":"Stewart","sequence":"additional","affiliation":[{"name":"Lexar Laboratories Lexington KY USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Virginia D.","family":"Barker","sequence":"additional","affiliation":[{"name":"Maxwell H. Gluck Equine Research Center Department of Veterinary Science University of Kentucky Lexington KY USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Amanda A.","family":"Adams","sequence":"additional","affiliation":[{"name":"Maxwell H. Gluck Equine Research Center Department of Veterinary Science University of Kentucky Lexington KY USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8942-6803","authenticated-orcid":false,"given":"Martin K.","family":"Nielsen","sequence":"additional","affiliation":[{"name":"Maxwell H. 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