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In this study, PGE<jats:sub>2<\/jats:sub> and PGE<jats:sub>2<\/jats:sub> receptor levels and the regulatory effect of PGE<jats:sub>2<\/jats:sub> on arginase activity, NO<jats:sub>2<\/jats:sub>, IL\u201010, IL\u201017, IFN\u2010\u03b3, TNF\u2010\u03b1 and parasite load were evaluated in cultures of splenic leucocytes obtained from dogs with CanL in the presence of agonists and inhibitors. Our results showed that splenic leucocytes from dogs with CanL had lower EP2 receptor levels than those of splenic leucocytes from healthy animals<jats:italic>.<\/jats:italic> We observed that NO<jats:sub>2<\/jats:sub> levels decreased when the cells were treated with a PGE<jats:sub>2<\/jats:sub> receptor agonist (EP1\/EP2\/EP3) or COX\u20102 inhibitor (NS\u2010398) and that TNF\u2010\u03b1, IL\u201017 and IFN\u2010\u03b3 cytokine levels decreased when the cells were treated with a PGE<jats:sub>2<\/jats:sub> receptor agonist (EP2) or PGE<jats:sub>2<\/jats:sub> itself. The parasite load in splenic leucocyte cell cultures from dogs with CanL decreased after stimulation of the cells with PGE<jats:sub>2<\/jats:sub>. We conclude that <jats:italic>Leishmania<\/jats:italic> infection of dogs modulates PGE<jats:sub>2<\/jats:sub> receptors and speculate that the binding of PGE<jats:sub>2<\/jats:sub> to its receptors may activate the microbicidal capacity of cells.<\/jats:p>","DOI":"10.1111\/pim.12713","type":"journal-article","created":{"date-parts":[[2020,3,16]],"date-time":"2020-03-16T08:49:55Z","timestamp":1584348595000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Regulatory effect of PGE<sub>2<\/sub> on microbicidal activity and inflammatory cytokines in canine leishmaniasis"],"prefix":"10.1111","volume":"42","author":[{"given":"Gabriela Lovizutto","family":"Venturin","sequence":"first","affiliation":[{"name":"School of Veterinary Medicine (Faculdade de Medicina Veterin\u00e1ria; FMVA) Sao Paulo State University \u2018J\u00falio de Mesquita Filho\u2019(UNESP) Ara\u00e7atuba Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jaqueline Poleto","family":"Bragato","sequence":"additional","affiliation":[{"name":"School of Veterinary Medicine (Faculdade de Medicina Veterin\u00e1ria; 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