{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,21]],"date-time":"2026-02-21T11:08:02Z","timestamp":1771672082504,"version":"3.50.1"},"reference-count":76,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2020,4,28]],"date-time":"2020-04-28T00:00:00Z","timestamp":1588032000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"UNAM DGAPA PAPIIT-UNAM","award":["IA205317"],"award-info":[{"award-number":["IA205317"]}]},{"name":"IPN-SIP","award":["20190012"],"award-info":[{"award-number":["20190012"]}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Parasite Immunology"],"published-print":{"date-parts":[[2020,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Many pathogenicity factors are involved in the development of primary amoebic meningoencephalitis (PAM) caused by <jats:italic>N\u00a0fowleri<\/jats:italic>. However, most of them are not exclusive for <jats:italic>N\u00a0fowleri<\/jats:italic> and they have not even been described in other nonpathogenic <jats:italic>Naegleria<\/jats:italic> species. Therefore, the objective of this work was to identify differential proteins and protein pattern recognition between <jats:italic>Naegleria fowleri<\/jats:italic> and <jats:italic>Naegleria lovaniensis<\/jats:italic> using antibodies anti\u2010<jats:italic>N\u00a0fowleri<\/jats:italic> as strategy to find vaccine candidates against meningoencephalitis. Electrophoresis and Western blots conventional and 2\u2010DE were performed for the identification of antigenic proteins, and these were analysed by the mass spectrometry technique. The results obtained in 2\u2010DE gels and Western blot showed very notable differences in spot intensity between these two species, specifically those with relative molecular weight of 100, 75, 50 and 19\u00a0kDa. Some spots corresponding to these molecular weights were identified as actin fragment, myosin II, heat shock protein, membrane protein Mp2CL5 among others, with differences in theoretical post\u2010translational modifications. In this work, we found differences in antigenic proteins between both species, proteins that could be used for a further development of vaccines against <jats:italic>N\u00a0fowleri<\/jats:italic> infection.<\/jats:p>","DOI":"10.1111\/pim.12715","type":"journal-article","created":{"date-parts":[[2020,3,19]],"date-time":"2020-03-19T18:35:01Z","timestamp":1584642901000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["Identification of differential protein recognition pattern between <i>Naegleria fowleri<\/i> and <i>Naegleria lovaniensis<\/i>"],"prefix":"10.1111","volume":"42","author":[{"given":"Mara","family":"Guti\u00e9rrez\u2010S\u00e1nchez","sequence":"first","affiliation":[{"name":"Laboratorio de Inmunobiolog\u00eda Molecular y Celular Secci\u00f3n de Estudios de Posgrado e Investigaci\u00f3n Escuela Superior de Medicina Instituto Polit\u00e9cnico Nacional M\u00e9xico City Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria Maricela","family":"Carrasco\u2010Yepez","sequence":"additional","affiliation":[{"name":"Laboratorio de Microbiolog\u00eda, Grupo CyMA Unidad de Investigaci\u00f3n Interdisciplinaria en Ciencias de la Salud y la Educaci\u00f3n Universidad Nacional Aut\u00f3noma de M\u00e9xico UNAM FES Iztacala Tlalnepantla Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jorge","family":"Herrera\u2010D\u00edaz","sequence":"additional","affiliation":[{"name":"Unidad de Servicios de Apoyo a la Investigaci\u00f3n y la Industria Facultad de Qu\u00edmica Universidad Nacional Aut\u00f3noma de M\u00e9xico Ciudad de M\u00e9xico Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Saul","family":"Rojas\u2010Hern\u00e1ndez","sequence":"additional","affiliation":[{"name":"Laboratorio de Inmunobiolog\u00eda Molecular y Celular Secci\u00f3n de Estudios de Posgrado e Investigaci\u00f3n Escuela 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