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Rhoptry organelle proteins (ROPs) secreted by <jats:italic>T gondii<\/jats:italic> play critical roles in host invasion.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods and results<\/jats:title><jats:p>In this study, influenza virus\u2010like particles (VLPs) expressing <jats:italic>T gondii<\/jats:italic> ROP4 or ROP13 were generated to assess vaccination\u2010induced changes in intracranial pro\u2010inflammatory cytokines and antibody responses upon <jats:italic>T gondii<\/jats:italic> challenge infection. Compared to ROP13 VLPs, ROP4VLPs vaccination significantly limited the production of pro\u2010inflammatory cytokines IFN\u2010\u03b3 and IL\u20106 in the brains of mice. Reduced pro\u2010inflammatory cytokine responses by ROP4 VLPs and ROP13 VLPs correlated with significantly increased <jats:italic>T gondii<\/jats:italic>\u2010specific IgG and IgA antibody responses in the brain, as well as IgG, IgG1 and IgM antibody responses in the sera.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>We concluded that influenza <jats:italic>T gondii<\/jats:italic> VLP vaccination induces antibody responses in sera and brain, which may contribute to the significant reduction of neuroinflammation during <jats:italic>T gondii<\/jats:italic> infection.<\/jats:p><\/jats:sec>","DOI":"10.1111\/pim.12716","type":"journal-article","created":{"date-parts":[[2020,4,6]],"date-time":"2020-04-06T11:32:17Z","timestamp":1586172737000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":22,"title":["<i>Toxoplasma gondii<\/i> virus\u2010like particle vaccination alleviates inflammatory response in the brain upon <i>T\u00a0gondii<\/i> infection"],"prefix":"10.1111","volume":"42","author":[{"given":"Hae\u2010Ji","family":"Kang","sequence":"first","affiliation":[{"name":"Department of Biomedical Science Graduate School Kyung Hee University Seoul Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ki\u2010Back","family":"Chu","sequence":"additional","affiliation":[{"name":"Department of Biomedical Science Graduate School Kyung Hee University Seoul Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Su\u2010Hwa","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Biomedical Science Graduate School Kyung Hee University Seoul Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Min\u2010Ju","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Biomedical Science Graduate School Kyung Hee University Seoul 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